Mitogen-activated protein kinase (MAPK) pathways mediate embryonic responses to culture medium osmolarity by regulating Aquaporin 3 and 9 expression and localization, as well as embryonic apoptosis.

BACKGROUND: In order to advance the development of culture conditions and increase the potential for supporting normal preimplantation embryo development in vitro, it is critical to define the mechanisms that early embryos utilize to survive in culture. We investigated the mechanisms that embryos employ in response to culture medium osmolarity. We hypothesized that mitogen-activated protein kinase (MAPK) pathways mediate responses to hyperosmotic stress by regulating Aquaporin (AQP) 3 and 9 expression as well as embryonic apoptosis. METHODS: Real-time reverse transcription and polymerase chain reaction and whole-mount immunofluorescence were used to determine the relative mRNA levels and protein localization patterns of AQP 3 and 9 after hyperosmotic medium treatment. RESULTS: At 6 and 24 h, a significant increase in Aqp 3 and 9 mRNA was observed in the sucrose hyperosmotic treatment compared with standard medium and glycerol controls. Blockade of MAPK14/11 negated the increase in Aqp 3 and 9 mRNA levels, whereas culture in a MAPK8 blocker did not. Hyperosmotic sucrose treatment significantly increased embryonic apoptosis which was negated in the presence of MAPK8 blocker, but not MAPK14/11 blocker. CONCLUSIONS: MAPK14/11 activation is a component of the rapid adaptive stress response mechanism that includes the effects of AQP mRNA expression and protein localization, whereas the MAPK8 pathway is a regulator of apoptosis

The effect of deletion of the orphan G – protein coupled receptor (GPCR) gene MrgE on pain-like behaviours in mice

<p>Abstract</p> <p>Background</p> <p>The orphan GPCR <it>MrgE </it>is one of an extended family of GPCRs that are expressed in dorsal root ganglia (DRG). Based on these expression patterns it has been suggested that GPCRs like <it>MrgE </it>may play a role in nociception however, to date, no direct supporting evidence has emerged. We generated mutant mice lacking <it>MrgE </it>and examined the effects of deletion of this gene in three pain behavioural models. The effect of <it>MrgE </it>gene deletion on expression of <it>Mrgs </it>and genes involved in sensory neurone function was also investigated.</p> <p>Results</p> <p>The absence of <it>MrgE </it>had no effect on the development of pain responses to a noxious chemical stimulus or an acute thermal stimulus. However, in contrast, the development but not the maintenance of neuropathic pain was affected by deletion of <it>MrgE</it>. The expression of <it>Mrg </it>genes was not significantly affected in the <it>MrgE </it>knockout (KO) mice with the sole exception of <it>MrgF</it>. In addition, the expression of 77 of 84 genes involved in sensory neuron development and function was also unaffected by deletion of <it>MrgE</it>. Of the 7 genes affected by <it>MrgE </it>deletion, 4 have previously been implicated in nociception.</p> <p>Conclusion</p> <p>The data suggests that <it>MrgE </it>may play a role in selective pain behavioural responses in mice.</p

The Formation of Massive Cluster Galaxies

We present composite 3.6 and 4.5 micron luminosity functions for cluster galaxies measured from the Spitzer Deep, Wide-Field Survey (SDWFS) for 0.3<z<2. We compare the evolution of m* for these luminosity functions to models for passively evolving stellar populations to constrain the primary epoch of star formation in massive cluster galaxies. At low redshifts (z < 1.3) our results agree well with models with no mass assembly and passively evolving stellar populations with a luminosity-weighted mean formation redshift zf=2.4 assuming a Kroupa initial mass function (IMF). We conduct a thorough investigation of systematic biases that might influence our results, and estimate systematic uncertainites of Delta zf=(+0.16-0.18) (model normalization), Delta zf=(+0.40-0.05) (alpha), and Delta zf=(+0.30-0.45) (choice of stellar population model). For a Salpeter type IMF, the typical formation epoch is thus strongly constrained to be z ~2-3. Higher formation redshifts can only be made consistent with the data if one permits an evolving IMF that is bottom-light at high redshift, as suggested by van Dokkum et al 2008. At high redshift (z > 1.3) we also witness a statistically significant (>5sigma) disagreement between the measured luminosity function and the continuation of the passive evolution model from lower redshifts. After considering potential systematic biases that might influence our highest redshift data points, we interpret the observed deviation as potential evidence for ongoing mass assembly at this epoch.Comment: 17 pages, 14 figures, accepted for publication in Ap

Evidence-Based Professional Development of Science Teachers in Two Countries

The focus of this collaborative research project of King?s College London, and the Weizmann Institute, Israel. project is on investigating the ways in which teachers can demonstrate accomplished teaching in a specific domain of science and on the teacher learning that is generated through continuing professional development programs (CPD) that lead towards such practice. The interest lies in what processes and inputs are required to help secondary school science teachers develop expertise in a specific aspect of science teaching. `It focuses on the design of the CPD programmes and examines the importance of an evidence-based approach through portfolioconstruction in which professional dialogue pathes the way for teacher learning. The set of papers highlight the need to set professional challenge while tailoring CPD to teachers? needs to create the environment in which teachers can advance and transform their practice. The cross-culture perspective added to the richness of the development and enabled the researchers to examine which aspects were fundamental to the design by considering similarities and differences between the domains

A Practical Guide to Climate-Smart Agriculture Technologies in Africa

Climate-smart agriculture (CSA) has been promoted since 2011 to increase productivity, improve resilience to climate variability and change and reduce greenhouse gas emission, where feasible, in farming systems globally and especially in Sub-Saharan Africa. CSA is unique, by comparison, to some other agricultural development approaches because it is outcome oriented, explicitly considers synergies and trade-offs among food and environment objectives and promotes solutions relevant to specific times and places. These advances however complicate CSA programming and investments. Such a flexible framework often leaves policy makers and program developers asking what is and what is not climate-smart? This guide provides a simple qualitative planning tool to help answer that question. With the information compiled here based on expert survey, users can conduct a rapid appraisal of the ‘climate-smartness’ of management practices and technologies. Specifically, users can explore suggested management practices and technologies based on (1) climate risks they address, (2) constraints to adoption and (3) potential impacts on productivity, resilience and mitigation when changing management of cereal-, paddy rice-, tree-, livestock- and fish-based systems. These three characteristics of risks, constraints and outcomes represent a minimum level of information to consider when deciding whether a technique is climate-smart or not and potential concerns or opportunities. The document concludes with a compilation of technical manuals and extension guides on practices to provide user instructions on implementing technologies in the field

Gaps in detailed knowledge of human papillomavirus (HPV) and the HPV vaccine among medical students in Scotland

&lt;p&gt;Background: A vaccination programme targeted against human papillomavirus (HPV) types16 and 18 was introduced in the UK in 2008, with the aim of decreasing incidence of cervical disease. Vaccine roll out to 12–13 year old girls with a catch-up programme for girls aged up to 17 years and 364 days was accompanied by a very comprehensive public health information (PHI) campaign which described the role of HPV in the development of cervical cancer.&lt;/p&gt; &lt;p&gt;Methods: A brief questionnaire, designed to assess acquisition of knowledge of HPV infection and its association to cervical cancer, was administered to two different cohorts of male and female 1st year medical students (school leavers: 83% in age range 17–20) at a UK university. The study was timed so that the first survey in 2008 immediately followed a summer's intensive PHI campaign and very shortly after vaccine roll-out (150 students). The second survey was exactly one year later over which time there was a sustained PHI campaign (213 students).&lt;/p&gt; &lt;p&gt;Results: We addressed three research questions: knowledge about three specific details of HPV infection that could be acquired from PHI, whether length of the PHI campaign and/or vaccination of females had any bearing on HPV knowledge, and knowledge differences between men and women regarding HPV. No female student in the 2008 cohort had completed the three-dose vaccine schedule compared to 58.4% of female students in 2009. Overall, participants’ knowledge regarding the sexually transmitted nature of HPV and its association with cervical cancer was high in both year groups. However, in both years, less than 50% of students correctly identified that HPV causes over 90% of cases of cervical cancer. Males gave fewer correct answers for these two details in 2009. In 2008 only around 50% of students recognised that the current vaccine protects against a limited subset of cervical cancer-causing HPV sub-types, although there was a significant increase in correct response among female students in the 2009 cohort compared to the 2008 cohort.&lt;/p&gt; &lt;p&gt;onclusions: This study highlights a lack of understanding regarding the extent of protection against cervical cancer conferred by the HPV vaccine, even among an educated population in the UK who could have a vested interest in acquiring such knowledge. The intensive PHI campaign accompanying the first year of HPV vaccination seemed to have little effect on knowledge over time. This is one of the first studies to assess detailed knowledge of HPV in both males and females. There is scope for continued improvements to PHI regarding the link between HPV infection and cervical cancer.&lt;/p&gt

SNAI1 and SNAI2 Are Asymmetrically Expressed at the 2-Cell Stage and Become Segregated to the TE in the Mouse Blastocyst

SNAI1 and SNAI2 are transcription factors that initiate Epithelial-to-Mesenchymal cell transitions throughout development and in cancer metastasis. Here we show novel expression of SNAI1 and SNAI2 throughout mouse preimplantation development revealing asymmetrical localization of both SNAI1 and SNAI2 in individual blastomeres beginning at the 2-cell stage through to the 8-cell stage where SNAI1 and SNAI2 are then only detected in outer cells and not inner cells of the blastocyst. This study implicates SNAI1 and SNAI2 in the lineage segregation of the trophectoderm and inner cell mass, and provides new insight into these oncogenes

“Not a good look”: impossible dilemmas for young women negotiating the culture of intoxication in the United Kingdom.

This paper investigates young women's alcohol consumption in the United Kingdom within a widespread culture of intoxication in relation to recent debates about postfeminism and contemporary femininity. Young women are faced with an “impossible dilemma,” arising from the contradiction between a hedonistic discourse of alcohol consumption and postfeminist discourse around attaining and maintaining the “right” form of hypersexual heterosexual femininity. Drawing on a recent interview study with 24 young white working-class and middle-class women in the South-West of England, we explore how young women inhabit the dilemmas of contemporary femininity in youth drinking cultures, striving to achieve the “right” form of hypersexual femininity and an “optimum” level of drunkenness