Cytotoxic activities of new iron(III) and nickel(II) chelates of some S-methyl-thiosemicarbazones on K562 and ECV304 cells

The S-methyl-thiosemicarbazones of the 2- hydroxy-R-benzaldehyde (R= H, 3-OH 3-OCH3 or 4-OCH3) reacted with the corresponding aldehydes in the presence of FeCl3 and NiCl2. New ONNO chelates of iron(III) and nickel (II) with hydroxy- or methoxy-substitued N1,N4-diarylidene-Smethyl- thiosemicarbazones were characterized by means of elemental analysis, conductivity and magnetic measurements, UV-Vis, IR and 1H-NMR spectroscopies. Cytotoxic activities of the compounds were determined using K562 chronic myeloid leukemia and ECV304 human endothelial cell lines by MTT assay. It was determined that monochloro N1-4- methoxysalicylidene-N4-4-methoxysalicylidene-S-methylthiosemicarbazidato- iron(III) complex showed selective anti-leukemic effects in K562 cells while has no effect in ECV304 cells in the 0.53 μg/ml (IC50) concentrations. Also, some methoxy-substitued nickel(II) chelates exhibit high cytotoxic activitiy against both of these cell lines in low concentrations. Cytotoxicity data were evaluated depending on cell lines origin and position of the substituents on aromatic rings

OTİZMİN GENETİK VE ÇEVRESEL TEMELLERİ HAKKINDAKİ SON ÇALIŞMALAR

Autism is a childhood neurodevelopmental disorder that affects 1–2 in 100 children, according to recent data on the broad array of autism spectrum disorders. It is a neurodevelopmental disorder, genetic and environmental factors playing role in autism. Molecular and mechanistic basis of autism started to be enlightened. As a genetic basis of disease, glutamate gene mechanisms supposed to develop a new method for diagnosing and treatment of autism. Aim of this mini-review is to gain a general knowledge about genetic and environmental reasons of autism

Effects of micro environmental factors on natural killer activity (NK) of beta thalassemia major patients

The physiological mechanisms of decreased NK activity of beta-Thalassemia major (BTM) patients are unknown. To assess in vitro effects of mononuclear cells and their cytokine secretion on NK activity, we compared activator receptor levels and cytotoxic activity of purified NK cells and NK cells in mononuclear cells (MNC) pools. We collected cell supernatant from unincubated and incubated MNC with K562 cells and measured their secreted cytokines levels. CD16 was lower on the surface of NK cells in MNC pools from BTM patients compared to healthy volunteers. This inhibition does not appear when NK cells were purified. NKp30 levels in NK cells decreased both as purified cells and as part of a pool of MNC in BTM patients. After incubation of MNC pools with K562 target cells, we found that supernatant levels of IL10, TGE beta 1 and IL15 cytokines were also significantly higher in BTM patients compared to healthy volunteers

Cytotoxic effect of extract from Dunaliella salina against SH-SY5Y neuroblastoma cells

Cytotoxic effects of essential oils extracted from Dunaliella sauna on SH-SY5Y human neuroblastoma cells were investigated in this study. GC-MS analysis was used for determination of the composition of essential oils found in Dunaliella salina extract. All experimented concentrations of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells were significantly more cytotoxic than the tested concentrations of the extract on ECV304 human endothelial cells used as a control. Fifthy compounds were detected in GC-MS analysis of the extract, and five major compounds were predominantly found as follows: octadecanoic acid, methyl ester (27.43%); hexadecanoic acid, methyl ester (Cas) methyl palmitate (24.82%); 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (7.39%); octadecanoic acid (5.03%), pentadecanoic acid (3.60%). The cytotoxic activity of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells might be due to high concentrations of octadecanoic acid and hexadecanoic acid. Furthermore, results indicate that the extract demonstrates some proliferative effect on ECV304 cells in a dose-dependent manner between 0.25 and 5 mu g/ml. These results suggest that Dunaliella sauna may have anticancer potential against human neuroblastoma cells

Decreased IFN-gamma and IL-12 levels in panic disorder

Objective: The aim of this study is to assess the measures of proinflammatory cytokines in patients with panic disorder in comparison with the healthy subjects. Methods: Twenty three patients with panic disorder with or without agoraphobia and twenty three controls were recruited for the study. Plasma samples of all subjects were analyzed for TNF-alpha, IFN-gamma, IL-1 beta, IL-2, IL-6, and IL-12 concentrations and NK-cell activity is measured in the peripheral blood samples of the subjects. Results: We found significant differences on the mean values of IL-12 (p = 0.01) and IFN-gamma (p = 0.02) between the panic disorder and control groups. In a logistic regression analysis, IFN-gamma values were significant statistical predictors of the presence of panic disorder (B = -0.07, SE = 0.03, p = 0.04). Conclusion: The most important implication of our results is to suggest a relation between panic disorder and low levels of IFN-gamma compatible with the results of the animal studies showing that IFN-gamma plays a role by acting to regulate the development of anxiety-like behaviors