3 research outputs found
The Erythrocyte Membrane Lipidomeof Healthy Dogs: Creating a Benchmark of Fatty Acid Distribution and Interval Values
Molecular-based approaches are rapidly developing in medicine for the evaluation of
physiological and pathological conditions and discovery of new biomarkers in prevention
and therapy. Fatty acid diversity and roles in health and disease in humans are topical
subjects of lipidomics. In particular, membrane fatty acid-based lipidomics provides
molecular data of relevance in the study of human chronic diseases, connecting
metabolic, and nutritional aspects to health conditions. In veterinarymedicine,membrane
lipidomics, and fatty acid profiles have not been developed yet in nutritional approaches
to health and in disease conditions. Using a protocol widely tested in human profiling,
in the present study erythrocyte membrane lipidome was examined in 68 clinically
healthy dogs, with different ages, sex, and sizes. In particular, a cluster composed
of 10 fatty acids, present in membrane glycerophospholipids and representative of
structural and functional properties of cell membrane, was chosen, and quantitatively
analyzed. The interval values and distribution for each fatty acid of the cluster were
determined, providing the first panel describing the healthy dog lipidomic membrane
profile, with interesting correlation to bodyweight increases. This molecular information
can be advantageously developed as benchmark in veterinarymedicine for the evaluation
of metabolic and nutritional status in healthy and diseased dogs