Propuesta de un nuevo modelo microquirúrgico para el estudio de la endometriosis inducida en rata Wistar. Resultados preliminares

The current knowledge status on the patogenesis of endometriosis as well as devastating consequences of disease evolution in women's reproductive health, have promoted researchers advances in a great manner during last years. The immunologic and neangiogenesis systems implication have opened new ways of knowledge over classic theories from the beginning of the xx century. The experimental resesearch, using animal induction models. Below we explain the first steps a new induction model ("PGR1-HotDog"), based on Wistar rats using a new disease autogeneration system, created for te study of the early stages of the endometriosis

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Propuesta de un nuevo modelo microquirúrgico para el estudio de la endometriosis inducida en rata Wistar. Resultados preliminares

The current knowledge status on the patogenesis of endometriosis as well as devastating consequences of disease evolution in women's reproductive health, have promoted researchers advances in a great manner during last years. The immunologic and neangiogenesis systems implication have opened new ways of knowledge over classic theories from the beginning of the xx century. The experimental resesearch, using animal induction models. Below we explain the first steps a new induction model ("PGR1-HotDog"), based on Wistar rats using a new disease autogeneration system, created for te study of the early stages of the endometriosis

Do Environmental Stream Classifications Support Flow Assessments in Mediterranean Basins?

The final publication is available at http://dx.doi.org/10.1007/s11269-012-0104-3Natural flow regimes are of primary interest in designing environmental flows and therefore essential for water management and planning. The present study discriminated natural hydrologic variation using two different environmental classifications (REC-Segura and WFD-ecotypes) and tested their agreement with an a posteriori (hydrologic) classification in a Spanish Mediterranean basin (the Segura River, SE Spain). The REC-Segura was developed as a two-level hierarchical classification based on environmental variables that influence hydrology (climate and source-of-flow). The WFD-ecotypes were developed by the Spanish Ministry for the Environment to implement the Water Framework Directive (WFD) using hierarchical hydrologic, morphologic and physicochemical variables. The climate level in the REC-Segura broadly described the hydrologic pattern observed along the NW-SE aridity gradient of the basin. However, source-of-flow (defined by karstic geology) was only able to discriminate variation in flow regimes within one climatic category. The WFD-ecotypes, despite incorporating hydrologic variables, did not fully discriminate hydrologic variation in the basin. Ecotypes in tributary streams located in dry or semiarid climates embrace different flow regimes (both perennial and intermittent). There was little agreement between environmental and hydrologic classifications. Therefore, the authors advise against the use of environmental classifications for the assessment of environmental flows without first testing their ability to discriminate hydrologic patterns.We would like to thank the University of Murcia for its financial support to Oscar Belmar through a pre-doctoral grant, the Hydrographic Confederation of the Segura for providing the precipitation data as well as the SIMPA model, and the Euromediterranean Institute of Water for its support to the project "Hydrological classification of the rivers and streams in the Segura Basin and associated macroinvertebrate communities".Belmar, O.; Velasco, J.; Martinez-Capel, F.; Peredo Parada, MM.; Snelder, T. (2012). Do Environmental Stream Classifications Support Flow Assessments in Mediterranean Basins?. Water Resources Management. 26(13):3803-3817. doi:10.1007/s11269-012-0104-3S38033817261

Hydrological classification of natural flow regimes to support environmental flow assessments in intensively regulated Mediterranean rivers, Segura River Basin (Spain)

The final publication is available at http://link.springer.com/article/10.1007%2Fs00267-011-9661-0Hydrological classification constitutes the first step of a new holistic framework for developing regional environmental flow criteria: the "Ecological Limits of Hydrologic Alteration (ELOHA)". The aim of this study was to develop a classification for 390 stream sections of the Segura River Basin based on 73 hydrological indices that characterize their natural flow regimes. The hydrological indices were calculated with 25 years of natural monthly flows (1980/81-2005/06) derived from a rainfall-runoff model developed by the Spanish Ministry of Environment and Public Works. These indices included, at a monthly or annual basis, measures of duration of droughts and central tendency and dispersion of flow magnitude (average, low and high flow conditions). Principal Component Analysis (PCA) indicated high redundancy among most hydrological indices, as well as two gradients: flow magnitude for mainstream rivers and temporal variability for tributary streams. A classification with eight flow-regime classes was chosen as the most easily interpretable in the Segura River Basin, which was supported by ANOSIM analyses. These classes can be simplified in 4 broader groups, with different seasonal discharge pattern: large rivers, perennial stable streams, perennial seasonal streams and intermittent and ephemeral streams. They showed a high degree of spatial cohesion, following a gradient associated with climatic aridity from NW to SE, and were well defined in terms of the fundamental variables in Mediterranean streams: magnitude and temporal variability of flows. Therefore, this classification is a fundamental tool to support water management and planning in the Segura River Basin. Future research will allow us to study the flow alteration-ecological response relationship for each river type, and set the basis to design scientifically credible environmental flows following the ELOHA framework. © 2011 Springer Science+Business Media, LLC.We wish to thank the University of Murcia for its financial support to Oscar Belmar by means of a pre-doctoral grant, the Euromediterranean Institute of Water for its support to the project "Hydrological classification of the rivers and streams in the Segura Basin and associated macroinvertebrate communities", the Hydrographic Confederation of the Segura for providing the SIMPA model and Ton Snelder and Matias Peredo-Parada for their valuable feedback on early drafts of this article.Belmar, O.; Velasco, J.; Martinez-Capel, F. (2011). Hydrological classification of natural flow regimes to support environmental flow assessments in intensively regulated Mediterranean rivers, Segura River Basin (Spain). Environmental Management. 47(5):992-1004. doi:10.1007/s00267-011-9661-0S992100447