27 research outputs found
Factors that transformed maize productivity in Ethiopia
Published online: 26 July 2015Maize became increasingly important in the food
security of Ethiopia following the major drought and famine
that occurred in 1984. More than 9 million smallholder house-
holds, more than for any other crop in the country, grow maize
in Ethiopia at present. Ethiopia has doubled its maize produc-
tivity and production in less than two decades. The yield,
currently estimated at >3 metric tons/ha, is the second highest
in Sub-Saharan Africa, after South Africa; yield gains for
Ethiopia grew at an annual rate of 68 kg/ha between 1990
and 2013, only second to South Africa and greater than
Mexico, China, or India. The maize area covered by improved
varieties in Ethiopia grew from 14 % in 2004 to 40 % in 2013,
and the application rate of mineral fertilizers from 16 to 34 kg/
ha during the same period. Ethiopia
’
s extension worker to
farmer ratio is 1:476, compared to 1:1000 for Kenya, 1:1603
for Malawi and 1:2500 for Tanzania. Increased use of im-
proved maize varieties and mineral fertilizers, coupled with
increased extension services and the absence of devastating
droughts are the key factors promoting the accelerated growth
in maize productivity in Ethiopia. Ethiopia took a homegrown
solutions approach to the research and development of its
maize and other commodities. The lesson from Ethiopia
’
s
experience with maize is that sustained investment in agricul-
tural research and development and policy support by the
national government are crucial for continued growth of
agricultur
CLONING, SEQUENCING AND EXPRESSION STUDIES OF THE GENES ENCODING AMICYANIN AND THE BETA-SUBUNIT OF METHYLAMINE DEHYDROGENASE FROM THIOBACILLUS-VERSUTUS
The genes encoding amicyanin and the beta-subunit of methylamine dehydrogenase (MADH) from Thiobacillus versutus have been cloned and sequenced. The organization of these genes makes it likely that they are coordinately expressed and it supports earlier findings that the blue copper protein amicyanin is involved in electron transport from methylamine to oxygen. The amino acid sequence deduced from the nucleotide sequence of the amicyanin-encoding gene is in agreement with the published protein sequence. The gene codes for a pre-protein with a 25-amino-acid-long signal peptide. The amicyanin gene could be expressed efficiently in Escherichia coli. The protein was extracted with the periplasmic fraction, indicating that pre-amicyanin is translocated across the inner membrane of E. coli. Sequence studies on the purified beta-subunit of MADH confirm the amino acid sequence deduced from the nucleotide sequence of the corresponding gene. The latter codes for a pre-protein with an unusually long (56 amino acids) leader peptide. The sequencing results strongly suggest that pyrroloquinoline quinone (PQQ) or pro-PQQ is not the co-factor of MADH
Isolation, Characterization, and Evolutionary Divergence of Mouse RNase 6: Evidence for Unusual Evolution in Rodents
The evolution of the ribonuclease A (RNase A) vertebrate-specific enzyme family is interesting in that specific gene lineages appear to be responding to unique selective pressures in wildly diverse manners to generate proteins that are capable of reducing the infectivity of viruses, killing systemic pathogens, and inducing the growth of blood vessels all while maintaining the signature motifs of a ribonuclease. In this paper, we present the DNA sequence and gene structure of Mus musculus RNase 6 and examine the expression pattern and enzymatic activity of the recombinant protein. M. musculus RNase 6 has a limited expression pattern compared to human RNase 6 and is an efficient ribonuclease, with a catalytic efficiency 17-fold higher than that of human protein. Evo- lutionary analysis reveals that RNase 6 was subject to unusual evolutionary forces ( d N / d S  = 1.2) in an ancestral rodent lineage before the separation of Mus and Rattus . However, more recent evolution of rodent RNase 6 has been relatively conserved, with an average d N / d S of 0.66. These data suggest that the ancestral rodent RNase 6 was subject to accelerated evolution, resulting in the conserved modern gene, which most likely plays an important role in mouse physiology.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/48058/1/239_2004_Article_2657.pd