Mesenchymal stem cells applied to the inflammatory and proliferative phases of wound healing

A cicatrização de feridas é um processo que requer a interação de várias células da derme e epiderme. O objetivo deste trabalho foi avaliar qual o momento da aplicação das células das ADSCs em feridas cutâneas agudas que faria diferença na cicatrização nos primeiros sete dias da lesão. As células-tronco foram isoladas do tecido adiposo de camundongos C57Bl/6 GFP+. Para tanto, foram utilizados 49 camundongos C57Bl/6, divididos em quatro grupos: grupo I (GI/controle; n=14); grupo II (GII; n=14): ADSCs injetadas no d0; grupo III (GIII; n=14): ADSCs injetadas no terceiro dia; e Grupo IV (GIV; n=7): ADSCs injetadas no quinto dia. As avaliações clínicas ocorreram nos dias zero, três, cinco e sete, e as histopatológicas nos dias cinco e sete. Na metodologia proposta, foi observado que o uso de ADSCs aumenta a vascularização, a formação de tecido de granulação, a colagenização e incrementa o número de folículos pilosos em apenas sete dias de avaliação. Além disso, o momento da aplicação das células não repercutiu diferenças significativas nas fases inflamatória e proliferativa do processo de cicatrização das feridas cutâneas.Wound healing is a process that requires the interaction of various cells in the dermis and epidermis. The aim of this study was to evaluate the action of ADSCs in the treatment of acute wounds in order to understand if application time of the cells results in a difference in healing the first seven days of injury. The stem cells were isolated from adipose tissue of C57BL / 6 mice GFP +. Thus, we used 49 mice C57BL / 6 divided into four groups: Group I (GI / control, n=14); Group II (GII; n=14): ADSCs injected to the d0; Group III (GIII; n=14): ADSCs injected on the 3rd day, and Group IV (GIV; n=7): ADSCs injected day 5(d5). Clinical evaluations were performed on days 0, 3, 5 and 7 and the histopathology on days 5 and 7. In the proposed methodology, the use of ADSCs increased vascularization, formation of granulation tissue, collagen deposition and increases the number of hair follicles in just seven days of evaluation. In addition, the time of application of the cells did not affect significant differences in the inflammatory and the proliferative phase of wound healing skin

Comparação entre as técnicas de substituição do ligamento redondo com implante de fáscia lata de bubalino e o pino transarticular na estabilização de luxação coxofemural em cães

02

Comparação entre as técnicas de substituição do ligamento redondo com implante de fáscia lata de bubalino e o pino transarticular na estabilização de luxação coxofemural em cães

02

Electrical stimulation in experimental wound healing in rabbits

Feridas cutâneas crônicas e complicadas são frequentemente encontradas na rotina da medicina veterinária e, muitas vezes, transformam os tratamentos em verdadeiro desafi o. A eletroterapia tem sido indicada como alternativa aos tratamentos convencionais, devido à sua capacidade de promover cicatrização. O objetivo deste trabalho é relatar um ensaio experimental utilizando a eletroestimulação (ES) na cicatrização de feridas cutâneas em coelhos. Para tanto, foram utilizados 10 coelhos Nova Zelândia, machos, hígidos, submetidos à anestesia geral para a indução experimental de duas feridas cutâneas de 1cm2 localizadas no dorso, caudal à borda das escápulas. As lesões foram higienizadas com solução salina 0,9%, sendo a do lado direito tratada por ES e a do lado esquerdo, como controle. Para ES utilizou-se a frequência de 60Hz, por 10 minutos a cada dois dias, sendo aplicadas quatro agulhas de acupuntura em pontos equidistantes ao redor da lesão, a 0,5cm da borda. Os animais foram divididos em dois grupos, de oito e de 15 dias, conforme o período de tratamento e avaliação macroscópica e histopatológica. Não houve diferença signifi cativa no tempo de cicatrização das lesões tratadas com ES. Porém, apresentaram qualidade tecidual, tanto na avaliação macro quanto microscópica, superior às do grupo controle. Com isso, é possível indicar a ES como tratamento de feridas cutâneas, por ser uma técnica efi ciente, de fácil aplicação e de relativo baixo custo.Chronic skin wounds are frequently found in veterinary medicine routine and it becomes a challenge for clinicians. Sometimes the treatment routinely used end up failing. The electrotherapy has been indicated as an alternative to conventional treatments because of its ability to promote healing. The aim of this paper is to report an experimental study using electrical stimulation (ES) in wound healing in rabbits. It was evaluated 10 healthy New Zealand rabbits that were submitted to general anesthesia for resection of two wounds with 1cm2 located in both sides of the back behind the scapula edge. It was determinate that the right wound would be treated by ES and the left would be used as a control, both cleaned with solution of NaCl 0,9%. The frequency used to ES was 60Hz for 10 minutes every two days, being applied by acupuncture needles in four equidistant points around the lesion, to 0.5cm from the edge. The animals were divided in one group of 15 days and another one of 8 days for treatment and macroscopic and histopathological evaluation. The results showed that the lesions treated by ES do not have signifi cant difference in healing time. However, those treated had higher scarring macroscopic and microscopic quality than the control group. Therefore, it is possible to indicate the use of ES as a skin wounds treatment, whereas it was demonstrated to be an effective technique, easy to use and has relatively low cost

Electrical stimulation in experimental wound healing in rabbits

Feridas cutâneas crônicas e complicadas são frequentemente encontradas na rotina da medicina veterinária e, muitas vezes, transformam os tratamentos em verdadeiro desafi o. A eletroterapia tem sido indicada como alternativa aos tratamentos convencionais, devido à sua capacidade de promover cicatrização. O objetivo deste trabalho é relatar um ensaio experimental utilizando a eletroestimulação (ES) na cicatrização de feridas cutâneas em coelhos. Para tanto, foram utilizados 10 coelhos Nova Zelândia, machos, hígidos, submetidos à anestesia geral para a indução experimental de duas feridas cutâneas de 1cm2 localizadas no dorso, caudal à borda das escápulas. As lesões foram higienizadas com solução salina 0,9%, sendo a do lado direito tratada por ES e a do lado esquerdo, como controle. Para ES utilizou-se a frequência de 60Hz, por 10 minutos a cada dois dias, sendo aplicadas quatro agulhas de acupuntura em pontos equidistantes ao redor da lesão, a 0,5cm da borda. Os animais foram divididos em dois grupos, de oito e de 15 dias, conforme o período de tratamento e avaliação macroscópica e histopatológica. Não houve diferença signifi cativa no tempo de cicatrização das lesões tratadas com ES. Porém, apresentaram qualidade tecidual, tanto na avaliação macro quanto microscópica, superior às do grupo controle. Com isso, é possível indicar a ES como tratamento de feridas cutâneas, por ser uma técnica efi ciente, de fácil aplicação e de relativo baixo custo.Chronic skin wounds are frequently found in veterinary medicine routine and it becomes a challenge for clinicians. Sometimes the treatment routinely used end up failing. The electrotherapy has been indicated as an alternative to conventional treatments because of its ability to promote healing. The aim of this paper is to report an experimental study using electrical stimulation (ES) in wound healing in rabbits. It was evaluated 10 healthy New Zealand rabbits that were submitted to general anesthesia for resection of two wounds with 1cm2 located in both sides of the back behind the scapula edge. It was determinate that the right wound would be treated by ES and the left would be used as a control, both cleaned with solution of NaCl 0,9%. The frequency used to ES was 60Hz for 10 minutes every two days, being applied by acupuncture needles in four equidistant points around the lesion, to 0.5cm from the edge. The animals were divided in one group of 15 days and another one of 8 days for treatment and macroscopic and histopathological evaluation. The results showed that the lesions treated by ES do not have signifi cant difference in healing time. However, those treated had higher scarring macroscopic and microscopic quality than the control group. Therefore, it is possible to indicate the use of ES as a skin wounds treatment, whereas it was demonstrated to be an effective technique, easy to use and has relatively low cost

Eletroacupuncture treatment for wound in a cat

A cicatrização de feridas é um evento complexo, que envolve a interação de diversos componentes celulares e bioquímicos. Ocorre espontaneamente, sem intervenções externas, mas que, quando tratada através de artifícios benéficos, tende a ocorrer de forma mais rápida e com melhores resultados funcionais e estéticos. Pesquisas recentes comprovam a eficácia da eletroacupuntura em estimular a cicatrização de feridas induzidas experimentalmente em animais. O objetivo deste trabalho é relatar o efeito da eletroacupuntura para o tratamento de uma ferida em cicatrização por segunda intenção numa gata atendida no Hospital de Clínicas Veterinárias (HCV) da Universidade Federal do Rio Grande do Sul (UFRGS). Após a ressecção de neoplasia mamária, houve, no pós-operatório, necrose tecidual com deiscência de sutura, sendo indicado o tratamento por eletroacupuntura. O que consistiu na inserção de agulhas de acupuntura descartáveis de 0,30X30mm em pontos equidistantes a meio centímetro da borda da ferida, sendo estas, conectadas ao aparelho de eletroacupuntura a uma frequência de 60 Htz, pulso alternado assimétrico intermitente e uma corrente alternada, variando de 8 a 20μAmps, durante 10 minutos. Foram avaliadas características qualitativas e quantitativas da ferida. A lesão apresentou, gradualmente, evolução cicatricial quanto à sua extensão, presença de necrose, infecção e algia à manipulação. A última revisão foi feita aos 21 dias, quando pôde ser observada a cicatrização quase completa da ferida. O uso da eletroacupuntura demonstrou ser benéfico ao processo cicatricial, promover rápida reparação da lesão, favorecendo o restabelecimento da estrutura e função dos tecidos.The wound healing is a complex event involving the interaction of various cellular and biochemical components. Occurs spontaneously, without external interventions, but that when treated through artifacts, tends to occur faster and with better functional and aesthetic results. Recent research is being demonstrated the effectiveness of eletroacupuntura to promote healing on experimentally wounds. And those animals have a complete healing, without contamination and with higher tension than control animals. The aim of this study is to assess the effect of eletroacupuncture for the treatment of a secondary intention wound healing at a cat that was attended at the Hospital de Clínicas Veterinárias (HCV) of the Universidade Federal do Rio Grande do Sul (UFRGS). After resection of breast cancer have occurred sutures dehiscence and tissue necrosis in the post-surgery It was suggested the eletroacupuncture treatment for wound healing. It was inserted the acupuncture needles 0.30X30mm in equidistant points of the wound, and after it was being connected to the apparatus of the eletroacupuncture at frequency of 60 Htz, pulse and alternating asymmetric blinking an alternating current, ranging 8 to 20μAmps for 10 minutes. It was evaluated qualitative and quantitative characteristics of the wound. It was observed a gradual reduction of the wound size, the percentage of necrotic tissue, the signs of infection and exudation, the pain during wound manipulation. The last review was done at 21 days, when we can be seen the almost complete wound healing. The use of eletroacupuncture shown to be effective in accelerating the healing process, promoting rapid injury repair, leading to restoration of the structure and function of tissues

Experimental rhinoplasty using autogenous rib graft in dogs

O uso de costela autógena para correção de defeito criado no osso nasal em cães foi utilizado em 15 animais, separados em três grupos de pós-operatório, em que, após 30, 60 e 90 dias, finalizavam-se seus acompanhamentos. Cada grupo era composto de quatro animais enxertados e um animal-controle, no qual se utilizava o material do próprio defeito criado para coaptação. Os animais receberam monitoração clínica diária, e a integração do enxerto foi avaliada com exames radiográficos obtidos a cada 15 dias, quando se realizou o exame histológico. A técnica cirúrgica foi de fácil execução e possui praticidade em seu uso e foi suficiente para a correção de todos os defeitos criados. O efeito estético foi satisfatório, os exames radiográficos comprovaram a integração do enxerto, e a avaliação histológica evidenciou a presença de células compatíveis com a viabilização da técnica proposta.The use of autologous rib to correct an experimental nasal bone lesion in dogs was performed in 15 animals, distributed in three post-operatory groups, and after 30, 60, and 90 days their clinical monitoring were finished. Each group was composed of four grafted animals and a control, in which it was used the material collected from the defect for coaptation. The animals received daily clinical monitoring and the graft integration was evaluated with radiographic exams taken every 15 days when the histological exam was performed. The surgical technique was easy to perform and had practical use. It was sufficient to correct all created lesions. The aesthetical effect was satisfactory; the radiographic exams proved the graft integration and the histological evaluation showed the presence of cells compatible with the viability of the technique

Suino como modelo experimental na pesquisa biomédica: valores fisiológicos normais

Background: The use of animals in biomedical research is of great importance. It is fundamental for the development and improvement of new practices and technologies. The selection of the species must take into consideration the practicality of handling and the suitability for the experiment, and it must have low operational costs. Swine are important as an experimental model in several experiment areas, because the functionality of many of their organic systems is developed similarly as it is in humans. Swine are animals that become easily stressed. For that reason, their conditioning is necessary to allow the obtainment of data that is closer to normal parameters. This study aims at reporting the physiological values obtained from awake swine, under normal conditions and minimum stress. Materials, Methods & Results: The animals were maintained under ideal environmental conditions, and they were always handled and conditioned by the same persons. Twenty crossbred swine were evaluated, aged between 60 and 90 days and with a body mass ranging between 17 and 25 kg. The parameters considered were cardiac frequency (FC), hemoglobin oxygen saturation (pulse oxymetry – SPO2), respiratory frequency (fR), end tidal carbon dioxide (ETCO2), systolic (PAM), diastolic (PAD) and mean (PAM) arterial pressure and arterial blood gas values – pH, arterial pressure of CO2 (PACO2), arterial pressure of O2 (PAO2), total CO2 (TCO2), base excess (EB), bicarbonate (HCO3) and O2 saturation (SATO2). The experiment was developed in compliance with the Standards of Animal Ethics and Welfare recommended by the National Council on the Control of Animal Experiments and with Law 11.794 of October 2008, being further approved by the Ethics Committee of the Hospital de Clínicas de Porto Alegre. To obtain the FC, fR, ETCO2 and SPO2 values, 20 animal samples were used. FC presented mean values (± standard deviation) of 134 ± 20.67 beats per minute (bpm), showing a maximum value of 180 bpm and a minimum of 103 bpm. The mean fR was 24.5 ± 5.61 breaths per minute (bpm), with a maximum value of 34 bpm and a minimum of 14 bpm, and SPO2 was 96 ± 2.10 %. Moreover, the values obtained in the analysis of exhaled gases showed a ETCO2 at 44.6 ± 5.41 mmHg, with a maximum value of 53 mmHg and minimum value of 34 mmHg. The arterial pressure was obtained from 19 animals, yielding the following values: 135.8 ± 16.29 mmHg for systolic pressure, 81.6 ± 14.10 mmHg for diastolic pressure and 103.3 ± 12.65 mmHg for mean pressure. Samples from 18 animals were used to measure the arterial blood gas. The values obtained were as follows: 7.497 ± 0.019 for Mean pH , 41.7 ± 6.95 mmHg for partial O2 pressure, 90.65 ± 2.5 mmHg for partial CO2 pressure, 32.8 ± 2.18 mmol/L for total CO2, 7.57 ± 1.9 mmol/L for base excess, 31.5 ± 2.12 mmol/L for bicarbonate and 97.55 ± 0.64 for oxygen saturation. Discussion: The animals seemed submissive and able to be conditioned and therefore it was easy to obtain basal values. These data were slightly different from the values obtained in other studies, thus becoming more reliable to be used as parameters in exsperiments employing this species. There seem to be interspecies differences when one intends to make a comparison with human beings. Values such as O2 concentration in arterial blood are normally lower in swine, possibly due to a lower hemoglobin concentration and a higher body temperature in the species. Despite some differences, there are many physiological similarities with human beings, which allows for the conclusion that swine is a great species to be employed in the biomedical research

Intestinal Lymphangiectasia associated with lipogranulomatous lymphangitis in a Pit Bull breed dog

A linfangiectasia é uma enteropatia desperdiçadora de proteínas, que ocorre devido à drenagem ineficaz da rede linfática, podendo estar acompanhada por uma linfangite lipogranulomatosa, que é a reação ao material de vasos linfáticos que se romperam. Através dos sinais clínicos, que incluem diarréia crônica, efusões cavitárias, edema de membros e emagrecimento progressivo, em conjunto com achados histopatológicos, se obtém o diagnóstico. Este estudo tem como objetivo descrever os aspectos clínicos e patológicos de um caso de linfangiectasia intestinal associada à linfangite lipogranulomatosa em um cão macho, 14 meses de idade, da raça pit bull, que foi atendido no Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul. O animal apresentava aumento abdominal, dispnéia, anorexia e cansaço. Em punção abdominal e torácica, observou-se um líquido esbranquiçado classificado, após análise, como quilo. Optou-se, então, pela laparotomia exploratória para identificação de possíveis causas para a alteração, mostrando obstrução do linfonodo mesentérico e pequenos nódulos brancos e irregulares na serosa do intestino delgado. Foi instituído o tratamento com dieta hipocalórica; porém, o paciente acabou indo a óbito decorridas três semanas de tratamento. Aliando-se os sinais clínicos aos exames laboratoriais e achados de necropsia, chegou-se ao diagnóstico definitivo: linfangiectasia intestinal associada à linfangite lipogranulomatosa.The lymphangiectasia is a protein-losing enteropathy coming from a fainling of lynphatics vessels flow. Lipogranulomatous lymphangitis could occour together with lymphangiectasia after reactions by substances witch are present in lynphatics vessels. Clinical signs include chronic diarrhea, peritoneal and pleural effusion, edema of the limbs and progressive weight loss. The final diagnoses were based on clinical signs together with histological findings. The aim of this paper is to describe clinical and pathlogical findings in a case of intestinal lymphangiectasia associated with lipogranulomatous lymphangitis in a male, 14-months-old, pit bull bread dog was referred at Hospital de Clínicas Veterinárias on Universidade Federal do Rio Grande do Sul. The dog was presented with distended abdomen, dyspnea, anorexia and lethargy. At abdominal and thoracic punction were revealed a white fluid classified, after analyse, like chylo. At exploratory surgery a mesenteric lymphonode obstruction was seen and multiple small white lesions occurring along lymphatic vessels on the serosal surface of the small intestine. The treatment was done with low calorie diet. Three weeks later, the patient had died. The necropsy, clinical sings and case evolution, lead to intestinal lymphangiectasia with lipogranulomatous lymphangitis diagnosis