Analysis of anemia and iron supplementation among glioblastoma patients reveals sex-biased association between anemia and survival

The association between anemia and outcomes in glioblastoma patients is unclear. We analyzed data from 1346 histologically confirmed adult glioblastoma patients in the TriNetX Research Network. Median hemoglobin and hematocrit levels were quantified for 6 months following diagnosis and used to classify patients as anemic or non-anemic. Associations of anemia and iron supplementation of anemic patients with median overall survival (median-OS) were then studied. Among 1346 glioblastoma patients, 35.9% of male and 40.5% of female patients were classified as anemic using hemoglobin-based WHO guidelines. Among males, anemia was associated with reduced median-OS compared to matched non-anemic males using hemoglobin (HR 1.24; 95% CI 1.00-1.53) or hematocrit-based cutoffs (HR 1.28; 95% CI 1.03-1.59). Among females, anemia was not associated with median-OS using hemoglobin (HR 1.00; 95% CI 0.78-1.27) or hematocrit-based cutoffs (HR: 1.10; 95% CI 0.85-1.41). Iron supplementation of anemic females trended toward increased median-OS (HR 0.61; 95% CI 0.32-1.19) although failing to reach statistical significance whereas no significant association was found in anemic males (HR 0.85; 95% CI 0.41-1.75). Functional transferrin-binding assays confirmed sexually dimorphic binding in resected patient samples indicating underlying differences in iron biology. Anemia among glioblastoma patients exhibits a sex-specific association with survival

The Myxobacterial Antibiotic Myxovalargin: Biosynthesis, Structural Revision, Total Synthesis, and Molecular Characterization of Ribosomal Inhibition

Resistance of bacterial pathogens against antibiotics is declared by WHO as a major global health threat. As novel antibacterial agents are urgently needed, we re-assessed the broad-spectrum myxobacterial antibiotic myxovalargin and found it to be extremely potent against Mycobacterium tuberculosis. To ensure compound supply for further development, we studied myxovalargin biosynthesis in detail enabling production via fermentation of a native producer. Feeding experiments as well as functional genomics analysis suggested a structural revision, which was eventually corroborated by the development of a concise total synthesis. The ribosome was identified as the molecular target based on resistant mutant sequencing, and a cryo-EM structure revealed that myxovalargin binds within and completely occludes the exit tunnel, consistent with a mode of action to arrest translation during a late stage of translation initiation. These studies open avenues for structure-based scaffold improvement toward development as an antibacterial agent

Attainment grouping as self-fulfilling prophesy?:A mixed methods exploration of self-confidence and set level among Year 7 students

Within-school segregation of pupils by attainment remains prevalent, despite evidence that these practices detrimentally impact outcomes for those in low attainment groups. This article explores the hypothesis that ‘ability grouping’ by setting impacts pupil self-confidence, precipitating a self-fulfilling prophecy. Survey data from 11,546 11/12 year old pupils in ‘ability sets’, and individual interviews and focus groups with 66 pupils, are drawn upon to explore this hypothesis. We find a significant correlation between perceived set placement and self-confidence in the set subject. More importantly, we also find a correlation between set placement and general self-confidence in learning. Pupils’ qualitative responses illustrate how setting can promote self-fulfilling prophecy, and worrying evidence of internalisation of ‘ability’ labels among pupils

The myxobacterial antibiotic myxovalargin: Biosynthesis, structural revision, total synthesis and molecular characterization of ribosomal inhibition

Resistance of bacterial pathogens against antibiotics is declared by WHO as a major global health threat. As novel antibacterial agents are urgently needed, we re-assessed the broad-spectrum myxobacterial antibiotic myxovalargin and found it to be extremely potent against Mycobacterium tuberculosis. To ensure compound supply for further development we studied myxovalargin biosynthesis in detail enabling production via fermentation of a native producer. Feeding experiments as well as functional genomics analysis suggested a structural revision, which was eventually corroborated by development of a concise total synthesis. The ribosome was identified as the molecular target based on resistant mutant sequencing and a cryo-EM structure revealed that myxovalargin binds within and completely occludes the exit tunnel, consistent with a mode of action to arrest translation during a late stage of translation initiation. Pharmacokinetic and initial in vivo efficacy studies indicated that myxovalargin and analogues show potential for development as an antibacterial agent

Fanya Kaplan

Directed by: Brad Tucker ; Michael Gluzman, Screenplay: Wesley Wingo, Cinematography: Alan Melling, Original Score: Rolan Duvvury, Editing: Matt Bigelow ; James Murphy, Cast - Fanya Kaplan: Becky Tucker, Yuri: Michael Gluzman, Mikhail: Matt Perry, Cheka Officer: Nick Tecosky, Vladimir Lenin: Patrick Bradshaw, Suit #1: Ildar Musin, Suit #2: David Rutter, Suit #3: Rolan Duvvury, Firing Squad Officer: Brad Tucker, Crew - Chief Lighting Technician: Wryen Meek, Gaffer: Tamil Perisamy ; John Swisshelm ; Dave Lillethun, Best Boy: Ildar Musin, Camera Assistant: Amaris Gutierrez-Ray, Make up Artist: Becky Tucker, Costume Designer: Becky Tucker ; Brad Tucker ; Michael Gluzman, Sound Technician: Matt Bigelow, Boom Operator: Ildar Musin ; Tamil Perisamy ; Dave Lillethun, Translation: Yury Gluzman ; Michael Gluzman, Dialog Coach: Michael Gluzman ; Ildar Musin, Historical Consultant: Yury Gluzman, Foley Artist: Matt Bigelow, Assistant to Mrs. Tucker: John Swisshelm ; Jason King, Armorer: Alan Melling, "Varshavianka" Performed by: Yury Gluzman, Extras: Jonathan Diaz ; Michelle Vela ; Ignacio Becerra-Licha ; Emily Weigel ; Jason King ; Aaron May ; Nicole Rennalls ; James Murphy ; Tamil Perisamy ; Steven Sloan ; Troy Whyte ; James Ford ; Walker Kirkland ; Wryen Meek ; Dave Lillithun ; Terrill Turner, Special Thanks: Yury and Lyudmila Gluzman ; Alan Melling ; Travis Ekmark ; Walker Kirkland ; Drew Dickerson ; Wesley Wingo ; Wryen Meek ; Jason King ; Nick Tecosky ; John Swisshelm ; BuzzStudios ; Georgia Institute of Technology ; The City of Atlanta ; a 1.6.18 Films Production in coordination with: Xero Films ; Ey3 Soar Productions.Q & A session: http://hdl.handle.net/1853/14238Runtime: 06:35 minutesTake a trip back to revolutionary Russia, 1918. This Campus Moviefest award-winner revisits the fascinating story of Fanya Kaplan, a zealous socialist revolutionary, and her attempted assassination of Vladimir Leni