404 research outputs found
Thermodynamics of a Colloidal Particle in a Time-Dependent Non-Harmonic Potential
We study the motion of an overdamped colloidal particle in a time-dependent
non-harmonic potential. We demonstrate the first law-like balance between
applied work, exchanged heat, and internal energy on the level of a single
trajectory. The observed distribution of applied work is distinctly
non-Gaussian in good agreement with numerical calculations. Both the Jarzynski
relation and a detailed fluctuation theorem are verified with good accuracy
Tunability of Critical Casimir Interactions by Boundary Conditions
We experimentally demonstrate that critical Casimir forces in colloidal
systems can be continuously tuned by the choice of boundary conditions. The
interaction potential of a colloidal particle in a mixture of water and
2,6-lutidine has been measured above a substrate with a gradient in its
preferential adsorption properties for the mixture's components. We find that
the interaction potentials at constant temperature but different positions
relative to the gradient continuously change from attraction to repulsion. This
demonstrates that critical Casimir forces respond not only to minute
temperature changes but also to small changes in the surface properties.Comment: 4 figures;
http://www.iop.org/EJ/article/0295-5075/88/2/26001/epl_88_2_26001.htm
Measuring the equation of state of a hard-disc fluid
We use video microscopy to study a two-dimensional (2D) model fluid of
charged colloidal particles suspended in water and compute the pressure from
the measured particle configurations. Direct experimental control over the
particle density by means of optical tweezers allows the precise measurement of
pressure as a function of density. We compare our data with theoretical
predictions for the equation of state, the pair-correlation function and the
compressibility of a hard-disc fluid and find good agreement, both for the
fluid and the solid phase. In particular the location of the transition point
agrees well with results from Monte Carlo simulations.Comment: 7 pages, to appear in EPL, slightly corrected versio
Normal and lateral critical Casimir forces between colloids and patterned substrates
We study the normal and lateral effective critical Casimir forces acting on a
spherical colloid immersed in a critical binary solvent and close to a
chemically structured substrate with alternating adsorption preference. We
calculate the universal scaling function for the corresponding potential and
compare our results with recent experimental data [Soyka F., Zvyagolskaya O.,
Hertlein C., Helden L., and Bechinger C., Phys. Rev. Lett., 101, 208301
(2008)]. The experimental potentials are properly captured by our predictions
only by accounting for geometrical details of the substrate pattern for which,
according to our theory, critical Casimir forces turn out to be a sensitive
probe.Comment: 6 pages, 3 figure
Critical Casimir effect in classical binary liquid mixtures
If a fluctuating medium is confined, the ensuing perturbation of its
fluctuation spectrum generates Casimir-like effective forces acting on its
confining surfaces. Near a continuous phase transition of such a medium the
corresponding order parameter fluctuations occur on all length scales and
therefore close to the critical point this effect acquires a universal
character, i.e., to a large extent it is independent of the microscopic details
of the actual system. Accordingly it can be calculated theoretically by
studying suitable representative model systems.
We report on the direct measurement of critical Casimir forces by total
internal reflection microscopy (TIRM), with femto-Newton resolution. The
corresponding potentials are determined for individual colloidal particles
floating above a substrate under the action of the critical thermal noise in
the solvent medium, constituted by a binary liquid mixture of water and
2,6-lutidine near its lower consolute point. Depending on the relative
adsorption preferences of the colloid and substrate surfaces with respect to
the two components of the binary liquid mixture, we observe that, upon
approaching the critical point of the solvent, attractive or repulsive forces
emerge and supersede those prevailing away from it. Based on the knowledge of
the critical Casimir forces acting in film geometries within the Ising
universality class and with equal or opposing boundary conditions, we provide
the corresponding theoretical predictions for the sphere-planar wall geometry
of the experiment. The experimental data for the effective potential can be
interpreted consistently in terms of these predictions and a remarkable
quantitative agreement is observed.Comment: 30 pages, 17 figure
On the nature of long-range contributions to pair interactions between charged colloids in two dimensions
We perform a detailed analysis of solutions of the inverse problem applied to
experimentally measured two-dimensional radial distribution functions for
highly charged latex dispersions. The experiments are carried out at high
colloidal densities and under low-salt conditions. At the highest studied
densities, the extracted effective pair potentials contain long-range
attractive part. At the same time, we find that for the best distribution
functions available the range of stability of the solutions is limited by the
nearest neighbour distance between the colloidal particles. Moreover, the
measured pair distribution functions can be explained by purely repulsive pair
potentials contained in the stable part of the solution.Comment: 6 pages, 5 figure
Phase Transitions of Soft Disks in External Periodic Potentials: A Monte Carlo Study
The nature of freezing and melting transitions for a system of model colloids
interacting by a DLVO potential in a spatially periodic external potential is
studied using extensive Monte Carlo simulations. Detailed finite size scaling
analyses of various thermodynamic quantities like the order parameter, its
cumulants etc. are used to map the phase diagram of the system for various
values of the reduced screening length and the amplitude of the
external potential. We find clear indication of a reentrant liquid phase over a
significant region of the parameter space. Our simulations therefore show that
the system of soft disks behaves in a fashion similar to charge stabilized
colloids which are known to undergo an initial freezing, followed by a
re-melting transition as the amplitude of the imposed, modulating field
produced by crossed laser beams is steadily increased. Detailed analysis of our
data shows several features consistent with a recent dislocation unbinding
theory of laser induced melting
Trichogin GA IV Alignment and Oligomerization in Phospholipid Bilayers
The membrane interactions and structure of the natural antimicrobial peptide trichogin GA IV have been investigated by CD, ATR FTIR and solid\u2010state NMR spectroscopy. A model emerges in which the peptide preferentially aligns parallel to the membrane surface and forms dimeric and tetrameric assemblies at higher concentrations
Effects of antimicrobial peptides on membrane dynamics: A comparison of fluorescence and NMR experiments
Antimicrobial peptides (AMPs) represent a promising class of compounds to fight resistant infections. They are commonly thought to kill bacteria by perturbing the permeability of their cell membranes. However, bacterial killing requires a high coverage of the cell surface by bound peptides, at least in the case of cationic and amphipathic AMPs. Therefore, it is conceivable that peptide accumulation on the bacterial membranes might interfere with vital cellular functions also by perturbing bilayer dynamics, a hypothesis that has been termed "sand in the gearbox". Here we performed a systematic study of such possible effects, for two representative peptides (the cationic cathelicidin PMAP-23 and the peptaibol alamethicin), employing fluorescence and NMR spectroscopies. These approaches are commonly applied to characterize lipid order and dynamics, but sample different time-scales and could thus report on different membrane properties. In our case, fluorescence anisotropy measurements on liposomes labelled with probes localized at different depths in the bilayer showed that both peptides perturb membrane fluidity and order. Pyrene excimer-formation experiments showed a peptideinduced reduction in lipid lateral mobility. Finally, laurdan fluorescence indicated that peptide binding reduces water penetration below the headgroups region. Comparable effects were observed also in fluorescence experiments performed directly on live bacterial cells. By contrast, the fatty acyl chain order parameters detected by deuterium NMR spectroscopy remained virtually unaffected by addition of the peptides. The apparent discrepancy between the two techniques confirms previous sporadic observations and is discussed in terms of the different characteristic times of the two approaches. The perturbation of membrane dynamics in the ns timescale, indicated by the multiple fluorescence approaches reported here, could contribute to the antimicrobial activity of AMPs, by affecting the function of membrane proteins, which is strongly dependent on the physicochemical properties of the bilayer
Understanding depletion forces beyond entropy
The effective interaction energy of a colloidal sphere in a suspension
containing small amounts of non-ionic polymers and a flat glass surface has
been measured and calculated using total internal reflection microscopy (TIRM)
and a novel approach within density functional theory (DFT), respectively.
Quantitative agreement between experiment and theory demonstrates that the
resulting repulsive part of the depletion forces cannot be interpreted entirely
in terms of entropic arguments but that particularly at small distances
( 100 nm) attractive dispersion forces have to be taken into account
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