5 research outputs found
Agricultural Research and Development in Ethiopia
To review past and current agricultural research activities and access their impact on agricultural development in Ethiopia and discuss some challenges and opportunities that need to be addressed
Glutenin Composition, Quality Characteristics, and Agronomic Attributes of Durum Wheat Cultivars Released in Ethiopia
Eleven cultivars of durum wheat (Triticum durum L. var. durum
Desf.) were evaluated across five environments in Ethiopia for grain
yield, 1000 kernel weight, protein concentration, gluten strength,
mixing time, mixing height, colour and yellow berry to identify
desirable traits for breeding purposes. Gluten strength was measured by
the sodium dodecyl sulfate (SDS) sedimentation test. An electrophoretic
study of gliadin and glutenin proteins was undertaken to investigate
possible associations between these proteins and gluten strength.
Significant genotypic differences were observed for grain yield, 1000
kernel weight, protein content, SDS-sedimentation volume, yellowness
and yellow berry. Six high molecular weight (HMW) glutenin subunits
patterns were identified with the combination of null and 20 being the
most common. For Glu-B1, the alleles producing protein subunits of 20
and 7+8 were the most common. Alleles producing protein subunits of 6+8
were less frequently observed. Three cultivars had pattern LMW-1 while
the remaining eight cultivars had pattern LMW-2. The strongest gluten
strength corresponded to the mixed subunits 7+8/6+8 and 7+8/20,
followed by subunits 6+8 and 7+8. Subunit 20 was associated with the
lowest gluten strength. Pattern LMW-2 was strongly associated with
higher gluten strength compared to LMW-1. The effects of low molecular
weight (LMW) and HMW glutenin subunits were additive. In order to
develop high quality durum wheats, it would be useful to discard lines
with LMW-1 and HMW glutenin subunit 20 and to combine electrophoretic
analysis with the SDS-sedimentation test