A study of selected isoenzymes in sheep

The work described in this thesis involved a study of isoenzymes (multiple forms of enzymes arising from genetically determined differences in primary structure) in ovine tissues and serum. The study is divided into three major parts.The first part describes the technical problems encountered and the methods used to solve these problems in relation to separating the isoenzymes of lactate dehydrogenase, glucosephosphate isomerase, aldolase, aspartate aminotransferase and creatine kinase. Both isoelectric focusing and electrophoresis were investigated but only the latter proved suitable for the quantitative estimations described in parts two and three.In the second part, normal isoenzyme levels were established in ovine tissues and an extensive investigation, involving 151 sheep, was carried out to determine the effect of physiological parameters on serum isoenzyme levels. Time after parturition in ewes and age in lambs affected serum lactate dehydro¬ genase isoenzyme levels but sex and growth rate produced no effects of any clinical significance. In a separate experiment, the frequency and mode of inheritance of two genetic variants of glucosephosphate isomerase was investigated.In the third part, the serum and tissue levels of lactate dehydrogenase and creatine kinase were studied in respiratory and gastrointestinal tract disease. In chronic proliferative exudative pneumonia and in acute pasteurella pneumonia, no changes in serum isoenzyme levels were observed which were likely to be of diagnostic value, despite a marked increase in the total lactate dehydrogenase level and changes in the distribution of its isoenzymes in pneumonic lungs. In chronic gastrointestinal parasitism, changes in serum lactate dehydrogenase and creatine kinase isoenzyme levels attributable to damage to the intestinal tract caused by Trichostrongylus vitrinus were detectable and may be of value in following the course of experimental infections in the live sheep. In the acute and chronic stages of parasitic gastritis caused by Ostertagia circumcincta or Ha emonchus contortus, no diagnostically useful serum isoenzyme changes were observed. In acute ID. circumcincta infection, isoenzyme levels in serum, and lymph from the gastric lymph duct were measured. Lymph isoenzymes were no more sensitive than serum isoenzymes in detecting damage to the abomasal mucosa, suggesting that the isoenzymes may have been released from the mucosa into the lumen of the abomasum rather than into the circulation via the lymphatic drainageChanges in the lactate dehydrogenase isoenzyme distribution of the abomasal mucosa were observed in acute parasitic gastritis but these were not reflected in the serum isoenzyme levels

Special Censuses: Ruminating About Enumerating?

Information about how and why a city should conduct a special census

An EDI Transaction Set Development Lifecycle (TSDL): A Case Study in the Food Manufacturing Industry

Electronic Data Interchange (EDI) is widely regarded by organizations worldwide as the business standard for the timely and secure exchange of electronic business transactions in an expanding global marketplace. While numerous research efforts have explored how the adoption, implementation, and integration of this technology can impact an organization\u27s technical, organizational, and competitive environment - few studies have documented the process used by organizations to implement EDI transaction sets with trading partners. This paper advances an innovative lifecycle used by a food manufacturing company to successfully design, implement, and test an EDI transaction set. This paper also describes how this transaction set development lifecycle (TDSL) can be integrated into an organization\u27s standard systems development lifecycle (SDLC) to ensure the timely completion of IT projects. Finally, a discussion of the impact of the TDSL on the organization\u27s business practices is presented

First year experience (FYE): International students’ experiences

International students confront a range of challenges during their transition to living and studying in Australia. Despite these challenges over 80% of international students reported high satisfaction with their life and study within Australia. This qualitative study reports on the experiences of 53 first year international students at ECU. Participants were students from across a range of study areas who responded to an online or face to face survey. Participants were required to respond to four questions which were analysed thematically to provide a summary of their experiences. Survey questions included their positive and negative experiences of being a first year student at ECU and changes that they suggested be considered by the university. Thematic analysis revealed a range of issues, most of which were reflected in previous research, however a range of ECU specific issues were also revealed, for example, resources and timetabling. This introductory research has provided initial data for developing future research. The cognitive nature of the survey may have limited the responses of participants. It is recommended that future research consider interviews with international students to review social and emotional issues

Culture of equine fibroblast-like synoviocytes on synthetic tissue scaffolds towards meniscal tissue engineering: a preliminary cell-seeding study

Introduction. Tissue engineering is a new methodology for addressing meniscal injury or loss. Synovium may be an ideal source of cells for in vitro meniscal fibrocartilage formation, however, favorable in vitro culture conditions for synovium must be established in order to achieve this goal. The objective of this study was to determine cellularity, cell distribution, and extracellular matrix (ECM) formation of equine fibroblast-like synoviocytes (FLS) cultured on synthetic scaffolds, for potential application in synovium-based meniscal tissue engineering. Scaffolds included open-cell poly-L-lactic acid (OPLA) sponges and polyglycolic acid (PGA) scaffolds cultured in static and dynamic culture conditions, and PGA scaffolds coated in poly-L-lactic (PLLA) in dynamic culture conditions.Materials and Methods. Equine FLS were seeded on OPLA and PGA scaffolds, and cultured in a static environment or in a rotating bioreactor for 12 days. Equine FLS were also seeded on PGA scaffolds coated in 2% or 4% PLLA and cultured in a rotating bioreactor for 14 and 21 days. Three scaffolds from each group were fixed, sectioned and stained with Masson’s Trichrome, Safranin-O, and Hematoxylin and Eosin, and cell numbers and distribution were analyzed using computer image analysis. Three PGA and OPLA scaffolds from each culture condition were also analyzed for extracellular matrix (ECM) production via dimethylmethylene blue (sulfated glycosaminoglycan) assay and hydroxyproline (collagen) assay. PLLA coated PGA scaffolds were analyzed using double stranded DNA quantification as areflection of cellularity and confocal laser microscopy in a fluorescent cell viability assay.Results. The highest cellularity occurred in PGA constructs cultured in a rotating bioreactor, which also had a mean sulfated glycosaminoglycan content of 22.3 µg per scaffold. PGA constructs cultured in static conditions had the lowest cellularity. Cells had difficulty adhering to OPLA and the PLLA coating of PGA scaffolds; cellularity was inversely proportional to the concentration of PLLA used. PLLA coating did not prevent dissolution of the PGA scaffolds. All cell scaffold types and culture conditions produced non-uniform cellular distribution.Discussion/Conclusion. FLS-seeding of PGA scaffolds cultured in a rotating bioreactor resulted in the most optimal cell and matrix characteristics seen in this study. Cells grew only in the pores of the OPLA sponge, and could not adhere to the PLLA coating of PGA scaffold, due to the hydrophobic property of PLA. While PGA culture in a bioreactor produced measureable GAG, no culture technique produced visible collagen. For this reason, and due to the dissolution of PGA scaffolds, the culture conditions and scaffolds described here are not recommended for inducing fibrochondrogenesis in equine FLS for meniscal tissue engineering

Sea surface pCO2 and O2 dynamics in the partially ice-covered Arctic Ocean

Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 122 (2017): 1425–1438, doi:10.1002/2016JC012162.Understanding the physical and biogeochemical processes that control CO2 and dissolved oxygen (DO) dynamics in the Arctic Ocean (AO) is crucial for predicting future air-sea CO2 fluxes and ocean acidification. Past studies have primarily been conducted on the AO continental shelves during low-ice periods and we lack information on gas dynamics in the deep AO basins where ice typically inhibits contact with the atmosphere. To study these gas dynamics, in situ time-series data have been collected in the Canada Basin during late summer to autumn of 2012. Partial pressure of CO2 (pCO2), DO concentration, temperature, salinity, and chlorophyll-a fluorescence (Chl-a) were measured in the upper ocean in a range of sea ice states by two drifting instrument systems. Although the two systems were on average only 222 km apart, they experienced considerably different ice cover and external forcings during the 40–50 day periods when data were collected. The pCO2 levels at both locations were well below atmospheric saturation whereas DO was almost always slightly supersaturated. Modeling results suggest that air-sea gas exchange, net community production (NCP), and horizontal gradients were the main sources of pCO2 and DO variability in the sparsely ice-covered AO. In areas more densely covered by sea ice, horizontal gradients were the dominant source of variability, with no significant NCP in the surface mixed layer. If the AO reaches equilibrium with atmospheric CO2 as ice cover continues to decrease, aragonite saturation will drop from a present mean of 1.00 ± 0.02 to 0.86 ± 0.01.U.S. National Science Foundation Arctic Observing Network Grant Number: ARC-1107346 and ARC-08564792017-08-2

Nurses\u27 Alumnae Association Bulletin - Volume 6 Number 9

Remember the Relief Fund Welcome! Miss Childs Financial Report Calendar of Coming Events Lest You Forget! Attention Review of the Alumnae Association Meetings Institutional Staff Nurses\u27 Section Report of Staff Activities - 1947-1948 Private Duty Section The White Haven Division Barton Memorial Division Remember the Relief Fund Student Nurses\u27 Activities Jefferson Scores Again The Clara Melville Scholarship Fund Interesting Activities of the Nurses\u27 Home Committee of the Women\u27s Board Exclusive for Nurses Changes in the Maternity Division Gray Lady Musical Therapy Service Memorial Service Honoring Mrs. Bessie Dobson Altemus The Blood Donor Center The Hospital Pharmacy Medical College News Remember the Relief Fund Administrative Staff and Faculty of the School of Nursing Streptomycin Changes in the Staff at Jefferson Hospital Care of the Thoracic Surgical Patient Miscellaneous Items Marriages New Arrivals Deaths The Bulletin Committee Attention, Alumnae New Addresse

Autophagy proteins control goblet cell function by potentiating reactive oxygen species production

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/102240/1/embj2013233-reviewer_comments.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102240/2/embj2013233-sup-0001.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102240/3/embj2013233.pd

Marrow transplants from unrelated donors for patients with aplastic anemia: Minimum effective dose of total body irradiation

AbstractPatients with aplastic anemia who do not have suitably HLA-matched, related donors generally receive immunosuppressive treatment as first-line therapy and are considered for transplantation from an unrelated donor only if they fail to respond to immunosuppressive treatment. In this setting, rates of transplantation-related morbidity and mortality have been high. We conducted a prospective study to determine the minimal dose of total body irradiation (TBI) sufficient to achieve sustained engraftment when it is used in combination with 3 cycles of 30 mg/kg of antithymocyte globulin (ATG) and 4 cycles of 50 mg/kg of cyclophosphamide (CY). We also wanted to determine the tolerability and toxicity of the regimen. The starting dosage of TBI was 3 x 200 cGy given over 2 days following CY/ATG. The TBI dose was to be escalated in increments of 200 cGy if graft failure occurred in the absence of prohibitive toxicity, and de-escalated for toxicity in the absence of graft failure. Twenty-one female and 29 male patients aged 1.3 to 46.5 years (median age, 14.4 years) underwent transplantation at 14 medical centers. The time interval from diagnosis to transplantation was 2.8 to 264 months (median, 14.5 months). All patients had been transfused multiple times and all had received 1 to 11 courses (median, 4 courses) of immunosuppressive treatment and other modalities of treatment. In 38 cases, the donors were HLA-A, -B and -DR phenotypically matched with the patients, and, in 12 cases, the donor phenotype differed from that of the recipient by 1 HLA antigen. Recipients of mismatched transplants were considered separately for TBI dose modification, and this study is still ongoing. Seven patients did not tolerate ATG and were prepared with 6 x 200 cGy of TBI plus 120 mg/kg of CY. Of the HLA-matched recipients prepared with CY/ATG/TBI, all 20 who received 3 x 200 or 2 x 200 cGy of TBI achieved engraftment, and 10 are alive. Of the 13 patients who received 1 x 200 cGy of TBI, 1 failed to engraft, and 8 are alive. Each of 10 patients who received an HLA-nonidentical transplant achieved engraftment, and 3 of 6 who were given 3 x 200 cGy of TBI, and 4 of 4 who were given 2 x 200 cGy are alive. Pulmonary toxicity occurred in 8 of 30 patients who were given 3 x 200 or 2 x 200 cGy of TBI concurrently with ATG and CY at 200 mg/kg, and in 2 of 13 patients who received 1 x 200 cGy of TBI, a pattern that suggests a decrease in toxicity with TBI dose de-escalation. Overall, the highest probability of survival (73%) was observed among patients who underwent transplantation within 1 year of diagnosis, compared with patients who underwent transplantation after a longer period of disease. In addition, younger patients (aged < or = 20 years) were more likely to survive than older patients (aged > 20 years). Thus, for patients with an HLA-matched, unrelated donor, a TBI dose of 200 cGy (in combination with CY/ATG) was sufficient to allow for engraftment without inducing prohibitive toxicity. As in previous studies, patient age and pretransplantation disease duration remain important prognostic factors.Biol Blood Marrow Transplant 2001;7(4):208-15

Impact of Tumor Size on Probability of Pathologic Complete Response After Neoadjuvant Chemotherapy

BACKGROUND: The prospective Neoadjuvant Breast Symphony Trial (NBRST) study found that MammaPrint/BluePrint functional molecular subtype is superior to conventional immunohistochemistry/fluorescence in situ hybridization subtyping for predicting pathologic complete response (pCR) to neoadjuvant chemotherapy. The purpose of this substudy was to determine if the rate of pCR is affected by tumor size. METHODS: The NBRST study includes breast cancer patients who received neoadjuvant chemotherapy. MammaPrint/BluePrint subtyping classified patients into four molecular subgroups: Luminal A, Luminal B, HER2 (human epidermal growth factor receptor 2), and Basal type. Probability of pCR (ypT0/isN0) as a function of tumor size and molecular subgroup was evaluated. RESULTS: A total of 608 patients were evaluable with overall pCR rates of 28.5 %. Luminal A and B patients had significantly lower rates of pCR (6.1 and 8.7 %, respectively) than either basal (37.1 %) or HER2 (55.0 %) patients (p < 0.001). The probability of pCR significantly decreased with tumor size >5 cm [p = 0.022, odds ratio (OR) 0.58, 95 % confidence interval (CI) 0.36, 0.93]. This relationship was statistically significant in the Basal (p = 0.026, OR 0.46, 95 % CI 0.23, 0.91) and HER2 (p = 0.039, OR 0.36, 95 % CI 0.14, 0.95) subgroups. In multivariate logistic regression analyses, the dichotomized tumor size variable was not significant in any of the molecular subgroups. DISCUSSION: Even though tumor size would intuitively be a clinical determinant of pCR, the current analysis showed that the adjusted OR for tumor size was not statistically significant in any of the molecular subgroups. Factors significantly associated with pCR were PR status, grade, lymph node status, and BluePrint molecular subtyping, which had the strongest correlation