Circumventing antivector immunity: potential use of nonhuman adenoviral vectors

Adenoviruses are efficient gene delivery vectors based on their ability to transduce a wide variety of cell types and drive high-level transient transgene expression. While there have been advances in modifying human adenoviral (HAdV) vectors to increase their safety profile, there are still pitfalls that need to be further addressed. Preexisting humoral and cellular immunity against common HAdV serotypes limits the efficacy of gene transfer and duration of transgene expression. As an alternative, nonhuman AdV (NHAdV) vectors can circumvent neutralizing antibodies against HAdVs in immunized mice and monkeys and in human sera, suggesting that NHAdV vectors could circumvent preexisting humoral immunity against HAdVs in a clinical setting. Consequently, there has been an increased interest in developing NHAdV vectors for gene delivery in humans. In this review, we outline the recent advances and limitations of HAdV vectors for gene therapy and describe examples of NHAdV vectors focusing on their immunogenicity, tropism, and potential as effective gene therapy vehicles

Observation of exclusive DVCS in polarized electron beam asymmetry measurements

We report the first results of the beam spin asymmetry measured in the reaction e + p -> e + p + gamma at a beam energy of 4.25 GeV. A large asymmetry with a sin(phi) modulation is observed, as predicted for the interference term of Deeply Virtual Compton Scattering and the Bethe-Heitler process. The amplitude of this modulation is alpha = 0.202 +/- 0.028. In leading-order and leading-twist pQCD, the alpha is directly proportional to the imaginary part of the DVCS amplitude.Comment: 6 pages, 5 figure

Measurement of bovine body and scrotal temperature using implanted temperature sensitive radio transmitters, data loggers and infrared thermography

Synchronous and continuous measurement of body (BT) and scrotal temperature (ST) without adverse welfare or behavioural interference is essential for understanding thermoregulation of the bull testis. This study compared three technologies for their efficacy for long-term measurement of the relationship between BT and ST by means of (1) temperature sensitive radio transmitters (RT), (2) data loggers (DL) and (3) infrared imaging (IRI). After an initial pilot study on two bulls to establish a surgical protocol, RTs and DLs were implanted into the flank and mid-scrotum of six Wagyu bulls for between 29 and 49\ua0days. RT frequencies were scanned every 15\ua0min, whilst DLs logged every 30\ua0min. Infrared imaging of the body (flank) and scrotum of each bull was recorded hourly for one 24-h period and compared to RT and DL data. After a series of subsequent heat stress studies, bulls were castrated and testicular tissue samples processed for evidence of histopathology. Radio transmitters were less reliable than DLs; RTs lost >11\ua0% of data, whilst 11 of the 12 DLs had 0\ua0% data loss. IRI was only interpretable in 35.8\ua0% of images recorded. Pearson correlations between DL and RT were strong for both BT (r\ua0>\ua00.94, P\ua0\ua00.80, P\ua

Reexamining the Potential to Classify Lava Flows From the Fractality of Their Margins

Can fractal analysis of a lava flow's margin enable classification of the lava's morphologic type (e.g., pāhoehoe)? Such classifications would provide insights into the rheology and dynamics of the flow when it was emplaced. The potential to classify lava flows from remotely-sensed data would particularly benefit the analysis of flows that are inaccessible, including flows on other planetary bodies. The technique's current interpretive framework depends on three assumptions: (1) measured margin fractality is scale-invariant; (2) morphologic types can be uniquely distinguished based on measured margin fractality; and (3) modification of margin fractality by topography, including substrate slope and confinement, would be minimal or independently recognizable. We critically evaluate these assumptions at meter scales (1–10 m) using 15 field-collected flow margin intervals from a wide variety of morphologic types in Hawaiʻi, Iceland, and Idaho. Among the 12 margin intervals that satisfy the current framework's suitability criteria (e.g., geomorphic freshness, shallowly-sloped substrates), we show that five exhibit notably scale-dependent fractality and all five from lava types other than ‘a‘ā or pāhoehoe would be classified as one or both of those types at some scales. Additionally, an ‘a‘ā flow on a 15° slope (Mauna Ulu, Hawaiʻi) and a spiny pāhoehoe flow confined by a stream bank (Holuhraun, Iceland) exhibit significantly depressed fractalities but lack diagnostic signatures for these modifications. We therefore conclude that all three assumptions of the current framework are invalid at meter scales and propose a new framework to leverage the potential of the underlying fractal technique while acknowledging these complexities. © 2021. American Geophysical Union. All Rights Reserved.6 month embargo; first published: 25 March 2021This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]