Promoter methylation analysis of CDH1 and p14ARF genes in patients with urothelial bladder cancer

BACKGROUND/AIM: Urothelial bladder cancer arises from the accumulation of multiple epigenetic and genetic changes. We aimed to investigate the specificity and sensitivity of gene-specific promoter methylation of CDH1 and p14ARF genes in the early diagnosis of bladder cancer and compare those with other diagnostic tests in our population. PATIENTS AND METHODS: In the current study, 65 patients with urothelial bladder cancer and 35 controls without any history of cancer were recruited. Methylation profiles of CDH1 and p14ARF genes from tumor and urine samples were determined by methylation-specific polymerase chain reaction method. RESULTS: Methylation of CDH1 and p14ARF genes in tumor samples was 95.4% and 78.5%, respectively. The methylation frequencies were found to be 68.8% for CDH1 gene and 72.9% for p14ARF gene in urine samples. Sensitivities of CDH1, p14ARF and urine cytology were found to be 67.4%, 72.1% and 34.9%, respectively, while their specificities were 93.9%, 63.6% and 93.9%, respectively. CONCLUSION: Aberrant promoter methylation of CDH1 and p14ARF genes can be used to detect urothelial bladder cancer. In low-grade tumors, when compared with urine cytology, combined methylation analysis of CDH1 and p14ARF genes may not increase the sensitivity to identify malignant cells in urine samples

Пчелы трибы Anthophorini Dahlbom, 1835 (Hymenoptera: Apoidea: Apidae) Нахичеванской Автономной Республики Азербайджана

В энтомофауне Нахичеванской Автономной Республики выявлено 37 видов из 3 родов пчел трибы Anthophorini, найденных в разное время в пределах территории автономной республики. Род Amegilla представлен 3 подродами (Amegilla, Micramegilla, Zebramegilla) и 7 видами, род Anthophora 7 подродами (Anthophora, Caranthophora, Dasymegilla, Heliophila, Lophanthophora, Melea, Paramegilla, Petalosternon, Pyganthophora) и 28 видами, а род Hapropoda 2 видами. Anthophora bimaculata (Panzer, 1798), A. richaensis Alfken, 1938, A. gracilipes (Morawitz, 1872), A. harmalae Morawitz, 1878, A. meridionalis Fedtschenko, 1875 и A. podagra Lepeletier, 1841 впервые указаны для фауны Нахичеванской АР. Установлены обитаемые видами ландшафты, биотопы и источники их питания-энтомофильные растения. Виды трибы Anthophorini питаются на цветках видов растений 19 семейств флоры автономной республики. Большое предпочтение они дают цветкам растений семейств Asteraceae, Fabaceae и Lamiaceae

The fauna and ecology of wasps of the genus Tachysphex Kohl, 1883 (Hymenoptera: Apoidea: Crabronidae) in Nakhchivan Autonomous Republic of Azerbaijan

An annotated checklist containing 11 species in five species groups of the wasp genus Tachysphex that occur in the Nakhchivan Autonomous Republic is presented. Five species are recorded from Azerbaijan for the first time: Tachysphex ferrugineus Pulawski, 1967, T. nitidus (Spinola, 1806), T. pompiliformis (Panzer, 1803), T. mocsaryi Kohl, 1884 and T. costae (de Stefani Perez, 1882). Six species are recorded from Nakhchivan AR for the first time: Tachysphex consocius Kohl, 1892, T. fulvitarsis (A. Costa, 1867), T. helveticus Kohl, 1885, T. brevipennis Mercet, 1909, T. pulcher Pulawski, 1967, T. dignus Kohl, 1889. Data on landscape and habitat distributions of each species are given

1,4-Bis[2-(prop-1-en­yl)phen­oxy]butane

The mol­ecule of the title compound, C22H26O2, exhibits Ci mol­ecular symmetry with a crystallographic inversion centre at the mid-point of the central C—C bond. A kink in the mol­ecule is defined by the torsion angle of 66.7 (2)° about this central bond of the alkyl bridge

Thermo Luminescence Dating of Pottery Sample from Chukhur Gabala Archaeological Site in Gabala District of Azerbaijan

The age of fragments of the ancient pottery sample from Chukhur Gabala archeological site in Gabala district of Azerbaijan has been estimated by employing Thermoluminescence dating (TL) method. The annual dose rate was obtained using γ-spectrometer with a hyper-pure germanium detector and it was 2.741±0.121 mGy/year. The age of the sample was calculated by an additive dose method as 2230±510 years which are in line with the stratigraphically estimated age of this area

Динамическое моделирование движения воздушного судна в продольной плоскости в неспокойной атмосфере на участке подхода к аэропорту

Application of dynamic modeling method upon ECAST to assess reactions of aircraft of Airbus type to random atmospheric disturbances without the use of Monte-Carlo method is considered. Application of the method will determine the significance of aircraft reactions to these disturbances, which will help in predicting the flight safety level.Рассматривается применение метода динамического моделирования стохастических систем при оценке реакций самолетов типа Airbus на случайные атмосферные возмущения без использования метода Монте-Карло. Применение представленного метода позволит определить значимость реакций воздушных судов на эти возмущения, что поможет при прогнозировании уровня безопасности полетов

Лапароскопическая радикальная нефрэктомия с тромбэктомией из нижней полой вены I - III уровня: опыт одного центра и обзор литературы.

Objective. Radical nephrectomy with thrombectomy of the inferior vena cava is the preferred treatment for renal cell carcinoma with an tumor thrombosis. We describe our experience and presentreview of the literature evaluating the feasibility and safety of laparoscopic nephrectomy with inferior vena cava thrombectomy.Materials and methods. The study included 37 patients who underwent laparoscopic radical nephrectomy with level I–III thrombectomy for renal cell carcinoma in our institution from 2018 to 2021. We analyzed the clinical, radiographic, intraoperative, pathological and postoperative parameters of the patients. The literature was reviewed by the Medline search engine, PubMed, with a review of publications on laparoscopic radical nephrectomy with inferior vena cava levelI–IIIthrombectomy.Results. The mean operation time was 275 ± 60.1 min, the median blood loss was 450 ± 81.6 ml (≥50 % of the circulating blood volume – 32.4 %). Intraoperative complications were observed during 10 (27.0 %) operations. Postoperative complications developed in 29.7 % of patients and reached gradesIII–IV according to the Clavien–Dindo scale on 13.0 % ill. All patients are activated according to the fast track rehabilitation program. The average hospital stay was 5 days. A literature review identified clinical cases and small series demonstrating the technical feasibility and safety of laparoscopic radical nephrectomy with thrombectomy in selected patients.Conclusion. Laparoscopic radical nephrectomy with thrombectomy is a technically feasible approach in carefully selected patients with level I–III tumor thrombosis. Optimal patient selection, extensive experience in laparoscopy and specialized centers are essential for the safe use of thistechnique.Цель исследования – представить непосредственные результаты выполнения лапароскопической радикальной нефрэктомии с тромбэктомией из нижней полой вены I–III уровней в условиях одного онкоурологического стационара.Материалы и методы. В исследование были включены 37 пациентов, перенесших лапароскопическую радикальную нефрэктомию с тромбэктомией из нижней полой вены I–III уровней по поводу почечно-клеточного рака в НМИЦ онкологии им. Н.Н. Петрова в период с 2018 по 2021 г. Проанализированы клинические, рентгенографические, интраоперационные, патоморфологические и послеоперационные параметры пациентов. В системах Medline и PubMed выполнен поиск публикаций, посвященных лапароскопической радикальной нефрэктомии с тромбэктомией из нижней полой вены I–III уровней за последние 10 лет.Результаты. Среднее время операции составило 275 ± 60,1 мин, медиана объема кровопотери – 450 ± 81,6 мл (≥50 % объема циркулирующей крови – 32,4 %). Интраоперационные осложнения отмечены в 10 (27,0 %) случаях. Послеоперационные осложнения развились у 29,7 % пациентов и достигли III–IV степеней тяжести по шкале Clavien–Dindo у 13,0 % больных. Все пациенты активизированы по программе ускоренной реабилитации fast track. Средняя продолжительность пребывания в стационаре составила 5 сут. Обзор литературы выявил клинические случаи и небольшие серии, демонстрирующие техническую осуществимость и безопасность лапароскопической радикальной нефрэктомии с тромбэктомией у отдельных пациентов.Заключение. Лапароскопическая радикальная нефрэктомия с тромбэктомией – технически осуществимый подход у тщательно отобранных пациентов с опухолевым тромбом I–III уровней. Оптимальный отбор пациентов, обширный опыт лапароскопии и специализированные центры являются принципиальными элементами для безопасного применения этого метода

Original data of the European sourcebook of crime and criminal justice statistics 2021 (6th ed.)

The sixth edition, covering the years 2011 – 2016, has been published in 2021. This publication covers the data pertaining to the project. This is the sixth edition of a data collection initiative that started in 1993 under the umbrella of the Council of Europe and has been continued since 2000 by an international group of experts that created the European Sourcebook of Criminal Justice e.V.1 and is also a Working Group of the European Society of Criminology. These experts act as regional coordinators of a network of national correspondents whose contribution has been decisive in collecting and validating data on a variety of subjects from 42 countriespeer-reviewe

Microelements in sierozem soils of Nakhichevan Autonomous Republic and their influence of sugar beet yield

Results on study of sierozem soils properties in one of the most interesting natural Azerbaijan region – Nakhichevan Autonomous Republic are presented in article. The problems of soil geochemistry and microelements doses influence on sugur beet yield are discussed