The Electrophoretic Profile Myofibrillar Proteins Extracted From Camel Muscles, Kept in Various Modes

Changes in electrophoretic profiles of myofibrillar protein (MFP) in the Longissimus thoracis (LD) of young camels (2 to 4 years), preserved by refrigeration has been treated or not by lactic acid solution 4% or citric acid 1%, were followed during the post-mortem time at the following times: 1, 2, 4, 6, 8, 10, 12, 24 and 48 hours. The cold preservation for 48 hours has not shown any particular distinctions in the protein profiles of this muscle. Changes related to the type of treatment were recorded during the storage time. Proteolysis of the myofibrillar fraction was earlier in this muscle in the case of treatment with one of two solutions of organic acids used, particularly in the case of using lactic acid. Indeed, these changes have affected at the first hour after slaughter the proteolysis of the myofibrillar proteins. Fragments of low molecular weight (42, 36, 33, 26, 23, 18, 16, 14 and 13 kDa) have been identified. The electrophoretic analysis showed that during refrigeration, LD treated with a solution of lactic acid is more sensitive to disruption phenomena and muscle protein proteolysis that lots of this muscle that even in the case of preservation by refrigeration only or by refrigeratio

Highly efficient delivery of functional cargoes by the synergistic effect of GAG binding motifs and cell-penetrating peptides

Protein transduction domains (PTDs) are powerful nongenetic tools that allow intracellular delivery of conjugated cargoes to modify cell behavior. Their use in biomedicine has been hampered by inefficient delivery to nuclear and cytoplasmic targets. Here we overcame this deficiency by developing a series of novel fusion proteins that couple a membrane-docking peptide to heparan sulfate glycosaminoglycans (GAGs) with a PTD. We showed that this GET (GAG-binding enhanced transduction) system could deliver enzymes (Cre, neomycin phosphotransferase), transcription factors (NANOG, MYOD), antibodies, native proteins (cytochrome C), magnetic nanoparticles (MNPs), and nucleic acids [plasmid (p)DNA, modified (mod)RNA, and small inhibitory RNA] at efficiencies of up to two orders of magnitude higher than previously reported in cell types considered hard to transduce, such as mouse embryonic stem cells (mESCs), human ESCs (hESCs), and induced pluripotent stem cells (hiPSCs). This technology represents an efficient strategy for controlling cell labeling and directing cell fate or behavior that has broad applicability for basic research, disease modeling, and clinical application