230 research outputs found
[OII] emitters in the GOODS field at z~1.85: a homogeneous measure of evolving star formation
We present the results of a deep, near-infrared, narrow band imaging survey
at a central wavelength of 1.062 microns (FWHM=0.01 microns) in the GOODS-South
field using the ESO VLT instrument, HAWK-I. The data are used to carry out the
highest redshift search for [OII]3727 emission line galaxies to date. The
images reach an emission line flux limit (5 sigma) of 1.5 x 10^-17 erg cm^-2
s^-1, additionally making the survey the deepest of its kind at high redshift.
In this paper we identify a sample of [OII]3727 emission line objects at
redshift z~1.85 in a co-moving volume of ~4100 Mpc^3. Objects are selected
using an observed equivalent width (EW_obs) threshold of EW_obs = 50 angstroms.
The sample is used to derive the space density and constrain the luminosity
function of [OII] emitters at z=1.85. We find that the space density of objects
with observed [OII] luminosities in the range log(L_[OII]) > 41.74 erg s^-1 is
log(rho)=-2.45+/-0.14 Mpc^-3, a factor of 2 greater than the observed space
density of [OII] emitters reported at z~1.4. After accounting for completeness
and assuming an internal extinction correction of A_Halpha=1 mag (equivalent to
A_[OII]=1.87), we report a star formation rate density of rho* ~0.38+/-0.06
Msun yr^-1 Mpc^-3. We independently derive the dust extinction of the sample
using 24 micron fluxes and find a mean extinction of A_[OII]=0.98+/-0.11
magnitudes (A_Halpha=0.52). This is significantly lower than the A_Halpha=1
(A[OII]=1.86) mag value widely used in the literature. Finally we incorporate
this improved extinction correction into the star formation rate density
measurement and report rho*~0.24+/-0.06 Msun yr^-1 Mpc^-3.Comment: 11 pages, 10 figures, accepted for publication in MNRA
Sunyaev Zel'dovich Effect Observations of Strong Lensing Galaxy Clusters: Probing the Over-Concentration Problem
We have measured the Sunyaev Zel'dovich (SZ) effect for a sample of ten
strong lensing selected galaxy clusters using the Sunyaev Zel'dovich Array
(SZA). The SZA is sensitive to structures on spatial scales of a few
arcminutes, while the strong lensing mass modeling constrains the mass at small
scales (typically < 30"). Combining the two provides information about the
projected concentrations of the strong lensing clusters. The Einstein radii we
measure are twice as large as expected given the masses inferred from SZ
scaling relations. A Monte Carlo simulation indicates that a sample randomly
drawn from the expected distribution would have a larger median Einstein radius
than the observed clusters about 3% of the time. The implied overconcentration
has been noted in previous studies with smaller samples of lensing clusters. It
persists for this sample, with the caveat that this could result from a
systematic effect such as if the gas fractions of the strong lensing clusters
are substantially below what is expected.Comment: submitte
Variant signal peptides of vaccine antigen, FHbp, impair processing affecting surface localization and antibody-mediated killing in most meningococcal isolates
© Copyright © 2019 da Silva, Karlyshev, Oldfield, Wooldridge, Bayliss, Ryan and Griffin. Meningococcal lipoprotein, Factor H binding protein (FHbp), is the sole antigen of the Trumenba vaccine (Pfizer) and one of four antigens of the Bexsero vaccine (GSK) targeting Neisseria meningitidis serogroup B isolates. Lipidation of FHbp is assumed to occur for all isolates. We show in the majority of a collection of United Kingdom isolates (1742/1895) non-synonymous single nucleotide polymorphisms (SNPs) in the signal peptide (SP) of FHbp. A single SNP, common to all, alters a polar amino acid that abolishes processing: lipidation and SP cleavage. Whilst some of the FHbp precursor is retained in the cytoplasm due to reduced binding to SecA, remarkably some is translocated and further surface-localized by Slam. Thus we show Slam is not lipoprotein-specific. In a panel of isolates tested, the overall reduced surface localization of the precursor FHbp, compared to isolates with an intact SP, corresponded with decreased susceptibility to antibody-mediated killing. Our findings shed new light on the canonical pathway for lipoprotein processing and translocation of important relevance for lipoprotein-based vaccines in development and in particular for Trumenba
Defining endogenous TACC3–chTOG–clathrin–GTSE1 interactions at the mitotic spindle using induced relocalization
A multiprotein complex containing TACC3, clathrin and other proteins has been implicated in mitotic spindle stability. To disrupt this complex in an anti-cancer context, we need to understand its composition and how it interacts with microtubules. Induced relocalization of proteins in cells is a powerful way to analyze protein–protein interactions and, additionally, monitor where and when these interactions occur. We used CRISPR/Cas9 gene editing to add tandem FKBP–GFP tags to each complex member. The relocalization of endogenous tagged protein from the mitotic spindle to mitochondria and assessment of the effect on other proteins allowed us to establish that TACC3 and clathrin are core complex members and that chTOG (also known as CKAP5) and GTSE1 are ancillary to the complex, binding respectively to TACC3 and clathrin, but not each other. We also show that PIK3C2A, a clathrin-binding protein that was proposed to stabilize the TACC3–chTOG–clathrin–GTSE1 complex during mitosis, is not a member of the complex. This work establishes that targeting the TACC3–clathrin interface or their microtubule-binding sites are the two strategies most likely to disrupt spindle stability mediated by this multiprotein complex
Resolving Clumpy vs. Extended Ly- In Strongly Lensed, High-Redshift Ly- Emitters
We present six strongly gravitationally lensed Ly- Emitters (LAEs) at
with HST narrowband imaging isolating Ly-. Through complex
radiative transfer Ly- encodes information about the spatial
distribution and kinematics of the neutral hydrogen upon which it scatters. We
investigate the galaxy properties and Ly- morphologies of our sample.
Many previous studies of high-redshift LAEs have been limited in Ly-
spatial resolution. In this work we take advantage of high-resolution
Ly- imaging boosted by lensing magnification, allowing us to probe
sub-galactic scales that are otherwise inaccessible at these redshifts. We use
broadband imaging from HST (rest-frame UV) and Spitzer (rest-frame optical) in
SED fitting; providing estimates on the stellar masses (), stellar population ages ( Myr), and amounts of dust
(, statistically consistent with zero). We employ
non-parametric star-formation histories to probe the young stellar-populations
which create Ly-. We also examine the offsets between the Ly-
and stellar continuum, finding small upper limits of offsets ()
consistent with studies of low-redshift LAEs; indicating our galaxies are not
interacting or merging. Finally, we find a bimodality in our sample's
Ly- morphologies: clumpy and extended. We find a suggestive trend: our
LAEs with clumpy Ly- are generally younger than the LAEs with extended
Ly-, suggesting a possible correlation with age.Comment: 17 pages (2 for references), 5 figures, 6 table
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