3 research outputs found
Can Quercetin be an Option for Treatment of Spinal Cord Injury? An Experimental Study
AIM: To determine the neuroprotective functions of quercetin and compare them with methylprednisolone in an experimental spinal cord injury model in rats
Ratlarda Deneysel Spinal Kord Hasarlanma Modelinde Kersetinin Etkisinin Metilprednizolon ile Karşılaştırmalı Araştırılması
Can Quercetin be an Option for Treatment of Spinal Cord Injury? An Experimental Study
AIM: To determine the neuroprotective functions of quercetin and compare
them with methylprednisolone in an experimental spinal cord injury model
in rats.
MATERIAL and METHODS: Thirty male, Wistar rats were assigned to five
experimental groups: sham (n=6), trauma (n=6), methylprednisolone (n=6),
single dose quercetin (n=6), and multiple doses of quercetin (n=6). An
aneurysm clip compression method was used to produce spinal cord injury
at level T7-9 after performing a laminectomy. In the sham group, only a
laminectomy was performed. Clip compression was performed to the spinal
cord after laminectomy in the trauma group. For Group 3, a single dose
of intraperitoneal (ip) methylprednisolone (30mg/kg) was administered
after laminectomy and trauma. A single dose of ip quercetin (100mg/kg)
was administered after laminectomy and trauma in Group 4. For Group 5,
multiple doses of ip quercetin (100 mg/kg) were administered on the
first, second, and third days after laminectomy and trauma. Spinal cord
and serum samples were obtained to measure the levels of malondialdehyde
(MDA), nitric oxide (NO), total antioxidant levels (TAL) at the 72nd
hour. Neurofunctional examinations of all the rats according to Drummond
and Moore criteria and inclined-plane tests to evaluate functional
healing were performed. All rats were sacrificed via intracardiac blood
depletion after the procedure.
RESULTS: Quercetin and methylprednisolone both increased plasma and
tissue levels of NO and MDA, and decreased TAL, with a statistically
significant difference (p<0.05). NO and MDA levels in plasma and tissue
were significantly higher in the trauma group (Group 2) when compared to
the sham group (Group 1), and TAL levels were significantly lower
(p<0.05). There was a statistically significant increase in the
treatment group's inclined-plane test (p<0.05), while there was no
difference in motor examination evaluations.
CONCLUSION: The results of this experimental study suggest that
quercetin can be thought as an option of treatment in spinal cord
injury