Proteomic analysis of early responsive resistance proteins of wheat (Triticum aestivum) to yellow rust (Puccinia striiformis f. sp. tritici) using ProteomeLab PF2D

Wheat (Triticum aestivum L.) yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. To clarify the molecular details and components of the resistance response in wheat offers further possibilities to combat yellow rust. In this study, differentially regulated early response proteins in wheat leaves infected by Pst isolates were investigated by proteomic approaches. Total proteins extracts from leaves harvested at 24 hour post inoculation (hpi) were separated by two dimensional liquid chromatography system, ProteomeLab PF2D. Following PF2D analysis, six hundred and thirty-seven protein peaks were compared one by one between protein patterns obtained from pathogen- and mock-inoculated leaf tissue. Among those differentially expressed 33 proteins were identified in Pst-infected plants as compared with mock-inoculated controls by nanoLC-ESI-MS/MS. Six proteins were exhibited homology to fungal proteins. Two fungal proteins, including E3 ubiquitin protein ligase and Ubiquitin-like protein, are important members of ubiquitin-proteasome system which the importance of the its proteolytic function in regulating the virulence of pathogenic fungi has just been realized recently. Other identified 27 proteins were host proteins in response to Pst and classified in five groups based on their roles in diverse biological processes. The results indicated that identified defence related proteins such as pathogene related protein 1 and 4 (PR1, PR4), Glutathione S transferase (GST) are major component for systemic acquired resistance (SAR) which is one of the strong disease resistance form in plants and appears within several days following the initial pathogen attack

Effects of curcumin supplementation on liver metabolism in rats fed a high-fat diet: A proteomic approach

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Background: In the modern world, many individuals routinely consume diets rich in animal fat, leading to a high incidence ofobesity. Curcumin (diferuloylmethane, turmeric) extracted from the dried root of the rhizome Curcuma longa, is a popular Asian dietary spice used in curry. Curcumin is the most active component of turmeric, comprising 2-8% by weight. Proteomics is an important tool for elucidating the cellular response to various treatments including dietary factors

Cigarette dependence among males and females aged 50+ living in Izmir, Turkey

PubMed ID: 22126571Background: Smoking, the most important preventable cause of death overall, is also the leading cause of cancer-related mortality. Tobacco consumption has a direct or indirect relationship with most types of cancer. In Turkey, the second leading cause of death is cancer, with lung cancer the most prevalent. Objective: The aim of this study was to determine the level of cigarette dependence among men and women aged 50+, living in İzmir. Methods: This research is a descriptive and cross-sectional field study, carried out with the participation of a total of 2,497 subjects. Results: It was found that 48.8% of the participants used to be smokers, and 95.5% of the smoking participants had been smoking for at least 11 years at the time of study. 36.1% of the participants were being exposed to secondhand smoke at home, and almost one third of the smokers (29%) had a moderate level of dependence. Conclusions: Continuing education may help high-risk groups develop good habits for a healthier lifestyle such as smoking less and quitting smoking, thereby reducing the level of morbidity and mortality of most common types of cancer