Phylogenetic analysis of mitochondrial 12S ribosomal RNA gene sequence of Mongolian wild boars

Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags (provinces) were subjected to DNA sequencing. Determined sequences from 18 specimens were registered into the GenBank and accession numbers were obtained. In this study mitochondrial 12S rRNA gene sequences of Mongolian wild boars were analyzed with 36 complete sequences of 12S rRNA gene of wild boar (Sus scrofa) available at NCBI GenBank. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances and tree construction with 1000 bootstrapped replications were conducted using MEGA 6. Maximum likelihood trees were constructed by the HKY model. A maximum likelihood tree with 53 complete sequences of mitochondrial 12S rRNA gene of Sus scrofa was constructed. Mongolian sequences from the same and adjacent locations were clustered together. European sequences were clustered together, additionally two sequences from south western China and two sequences from south eastern China were also clustered. Additionally, 12S rRNA gene sequences of Mongolian Sus scrofa, located between Asian and European sequences suggesting geographical location of Mongolia, played an important role in the gene flow between Asian and European wild boar population

Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars

Genomic DNA was extracted from tissue specimens of wild boars (Sus scrofa) in 10 different locations Mongolia. D-loop part of genome was amplified by PCR and was subjected to DNA sequencing. Determined sequences from 18 specimens were registered with the GenBank and accession numbers were obtained. A total of 54 complete mitochondrial D-loop sequences of wild boars available with NCBI GenBank were taken as a reference for comparison with that of Mongolian wild boars. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances, and Maximum Likelihood (ML) phylogenetic tree construction with 1000 bootstrapped replications were conducted using MEGA X software. Maximum Likelihood trees were constructed by the Hasegawa-Kishino-Yano (HKY) model. The results of the study showed that geographic location played an important role in sequence divergence between wild boars from various locations. Most of them were grouped together according to their respective geographic locations, except for several individuals. It is highly likely that the Mongolian subpopulation of wild boars, such as S. scrofa raddianus and S. scrofa nigripes, have had the same ancestor. In order to fully evaluate the distribution, ecology, and biology of Mongolian wild boars, it is essential to compare supplemental gene sequences that can reveal phylogenetic differences from the populations in the neighboring areas, such as Russia, northeast China, and Kazakhstan. The results of this study will be useful and informative for the protecting and conserving of wild boars in Mongolia

ショウホウシ ケイセイ ニ トモナッテ ハツゲンスル シンキナ Lilium longiflorum イデンシ ノ トクチョウズケ

https://library.naist.jp/mylimedio/dllimedio/show.cgi?bookid=100042343&oldid=78419博士 (Doctor)バイオサイエンス (Bioscience)博第319号甲第319号博士（バイオサイエンス）奈良先端科学技術大学院大

In vitro Seed Germination and Callus Induction of Ferula ferulaeoides (Steud.) Korov. (Apiaceae)

Ferula ferulaeoides is a highly valuable medicinal plant native to Mongolia. In vitro seed germination effi ciency of F. ferulaeoides was low, and it required cold stratifi cation for longer than 21 days and exogenous application of the hormone gibberellic acid (GA3) for germination. Cotyledons, hypocotyls and roots of two week old seedlings were cultured on Murashige and Skoog (MS) media supplemented with diff erent auxins including 2,4-dichlorophenoxy acetic acid (2,4-D), napthalene acetic acid (NAA) and indole-acetic acid (IAA) and cytokinin 6-Benzylaminopurine (BAP). This experiment showed that combination of BAP (0.5 mg/L) and auxins IAA and 2, 4 D was the most effi cient media for callus initiation from in vitro germinated seedling explants. Callus was sub-cultured with 4 weeks interval on the same media as callus initiation for a long term maintenanc

Characterization of an Aquaporin Gene ZpPIP2 from Zygophyllum potaninii

Zygophyllum potaninii Maxim. is a medicinal plant, distributed in arid regions of southern Mongolia. Full length of a cDNA clone, which was identifi ed as a stress induced gene by suppression subtractive hybridization was obtained by 5’RACEPCR, and named ZpPIP2 as deduced amino acid sequence shows high homology to plant aquaporin PIP2. ZpPIP2 is expressed in leaf and stem under normal conditions, and it is accumulated in the root in response to drought stress in Z. potaninii

A new record of critically endangered Saussurea bogedaensis (Asteraceae) from Dzungarian Gobi, Mongolia

A species in the family Asteraceae, Saussurea bogedaensis, was newly described from Bogeda Mountain in Xinjiang, China and is a critically endangered species in China. Morphological and genetic characteristics confirm the presence of this species in Mongolia, as it was found in Baitag Bogd Mountain (in the Dzungarian Gobi). In addition, the distribution and conservation status of S. bogedaensis are provided