PHYLOGENETIC DIVERSITY OF SOME SMALL MAMMALS FROM SCIURUDAE FAMILY IN MONGOLIA

The squirrel family, Sciuridae, is one of the largest and widespread, ecologically common families of mammals. Due to the conspicuousness of this small mammal group with a big population, insufficient knowledge exists about its phylogenetic relationships. In this study, we identified 14 DNA sequences of mitochondrial cytochrome oxidase I gene in 6 species of Sciuridae and these sequences were compared with corresponding published COI gene sequences of the same species in Eurasia and determined their phylogenetic relationships by Neighbor-joining method

HAPLOTYPES AND ALLELIC FREQUENCIES OF 12 Y-STR LOCI IN MONGOLIAN AND KOREAN MALE GROUPS

Уг судалгааг “Монгол хүний Y хромосомын генетик мэдээллийн сан”, “Цэргийн албан хаагчдын генийн мэдээллийн сан бүрдүүлэх, туршилт судалгаа“ төслүүдийн хүрээнд хийж гүйцэтгэв

Phylogenetic analysis of mitochondrial 12S ribosomal RNA gene sequence of Mongolian wild boars

Specimens of Mongolian wild boar (Sus scrofa) from Arkhangai, Dornod, Zavkhan, Orkhon, Ovorkhangai, Selenge, Tuv, Khovd, Khuvsgul and Uvs aimags (provinces) were subjected to DNA sequencing. Determined sequences from 18 specimens were registered into the GenBank and accession numbers were obtained. In this study mitochondrial 12S rRNA gene sequences of Mongolian wild boars were analyzed with 36 complete sequences of 12S rRNA gene of wild boar (Sus scrofa) available at NCBI GenBank. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances and tree construction with 1000 bootstrapped replications were conducted using MEGA 6. Maximum likelihood trees were constructed by the HKY model. A maximum likelihood tree with 53 complete sequences of mitochondrial 12S rRNA gene of Sus scrofa was constructed. Mongolian sequences from the same and adjacent locations were clustered together. European sequences were clustered together, additionally two sequences from south western China and two sequences from south eastern China were also clustered. Additionally, 12S rRNA gene sequences of Mongolian Sus scrofa, located between Asian and European sequences suggesting geographical location of Mongolia, played an important role in the gene flow between Asian and European wild boar population

PHYLOGENETIC DIVERSITY OF SOME SMALL MAMMALS FROM <em>SCIURUDAE</em> FAMILY IN MONGOLIA

The squirrel family, Sciuridae, is one of the largest and widespread, ecologically common families of mammals. Due to the conspicuousness of this small mammal group with a big population, insufficient knowledge exists about its phylogenetic relationships. In this study, we identified 14 DNA sequences of mitochondrial cytochrome oxidase I gene in 6 species of Sciuridae and these sequences were compared with corresponding published COI gene sequences of the same species in Eurasia and determined their phylogenetic relationships by Neighbor-joining method

Tissue Culture and Micropropagation of Mongolian Licorice ( Glycyrrhiza uralensis Fisch.)

Mongolian licorice ( Glycyrrhiza uralensis Fisch.) is a pharmacologically important plant rich in flavonoids and saponins. For tissue culture, root and cotyledon explants from seedlings were used. Sterilized explants with one node were used for micropropagation. Half-strength Murasige-Skuge medium and Gamborg‘s B5 medium with different supplements were used for the induction of calluses and multiple shoots. Conditions for tissue culture and micropropagation of G. uralensis were determined

Phylogenetic analysis of a region of mitochondrial cox-1 as a DNA barcode marker sequence of Gazella subgutturosa (Goitered gazelle) in Mongolia

Gazella subgutturosa, a vulnerable species, is threatened by illegal hunting for meat and sport. The mitochondrial cytochrome c oxidase subunit 1 gene (cox-1) is used as a DNA marker to distinguish mammalian species for the investigation of illegal hunting. In this study, we sequenced a part of the cox-1 gene (709 bp) of six Mongolian G. subgutturosa individuals. Our DNA sequences were clustered in a clade of Gazella which is distinct from other clades of mammalian species in the phylogenetic tree. Our findings suggest that DNA sequences can be useful in the investigation of illegal hunting

The tiger genome and comparative analysis with lion and snow leopard genomes

Tigers and their close relatives (Panthera) are some of the world's most endangered species. Here we report the de novo assembly of an Amur tiger whole-genome sequence as well as the genomic sequences of a white Bengal tiger, African lion, white African lion and snow leopard. Through comparative genetic analyses of these genomes, we find genetic signatures that may reflect molecular adaptations consistent with the big cats' hypercarnivorous diet and muscle strength. We report a snow leopard-specific genetic determinant in EGLN1 (Met39>Lys39), which is likely to be associated with adaptation to high altitude. We also detect a TYR260G>A mutation likely responsible for the white lion coat colour. Tiger and cat genomes show similar repeat composition and an appreciably conserved synteny. Genomic data from the five big cats provide an invaluable resource for resolving easily identifiable phenotypes evident in very close, but distinct, species.close141