Study on genetic variability of Cassidula aurisfelis (snail) by random amplified polymorphic DNAs

The genetic variability among individuals of Cassidula aurisfelis from Setiu Wetland, Terengganu Darul Iman was examined by using the random amplified polymorphic DNA (RAPD) technique. Ten oligonucleotide primers were screened and three primers were selected (OPA 02, OPA 04 and OPA 10)to amplify DNA from five samples of C. aurisfelis from Setiu Wetland, Terengganu. A total of 28 RAPD bands (RAPDs) with 17 polymorphic bands (60.71%) with size ranging from 300 – 1750 bp were scored from the population. Genetic distance for samples ranges from 0.135 to 0.269. Similarity index of samples ranges from 0.7179 to 0.8649 (mean 0.7810 ± 0.0497)

Correlation between anthropometric indexes and risk factors of cardiovascular diseases among the elderly population in Amirkola

Background: The ageing of population is an increasing phenomenon worldwide. Cardiovascular diseases are one of the most important chronic disease in ageing. The aim of this study was to investigate the correlation between the anthropometric indexes and risk factors of cardiovascular diseases among the elderly population in Amirkola (Mazandaran, Iran). Materials and Methods: This descriptive-analytical cross-sectional study was a part of the Amirkola Health and Ageing Cohort Project (AHAP). Demographic information was collected using a standard questionnaire. Fasting blood samples were collected from all participants to measure the serum level of lipids. Measurement of systolic and diastolic blood pressure and anthropometric indexes e.g. body mass index (BMI), waist circumference (WC), waist to hip ratio (WHR) and waist to height ratio (WHR) were done for all participants. Results: One thousand five hundred and six elderly people of Amirkola (age> 60 years) were participated in this study. There was a positive and significant correlation between the BMI and systolic and diastolic blood pressures, total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and triglyceride. The WC had a positive and significant correlation with systolic and diastolic blood pressures, HDL-C and triglyceride. The correlation between WHR and systolic and diastolic blood pressures, HDL-C and triglyceride was positive and significant; however the correlation between the anthropometric indexes and TC and low density lipoprotein-cholesterol (LDL-C) was negative and significant. The WHR had a significant positive correlation with all studied risk factors. Conclusion: According to the findings of present study, WHR index had stronger correlation with risk factors for cardiovascular disease in the elderly

Heart Rate detection using Photoplethysmography using Android Phone

This paper includes Heart Rate Detector system implemented by some modern hardware ICs and simple sensor circuit with software executable on both PC and android platform. Very first the bio-signals are extracted via photoplethysmography concept using PPG sensor into electric signal. Now at the next stage microprocessor is used to convert the bio-signal from analog to digital format. Some application software running on Windows and Android phone have been developed to display heart rate information and time domain waveform to users for health care monitoring. Some of the applications running on the android platform few of them have been developed to display the heart rate information and some health care monitoring system. This project includes the RF modules which having the major impact and plays the predominant role in it. In future, pure wireless technology will be used instead of RF modules DOI: 10.17762/ijritcc2321-8169.15032

Haptoglobin frequencies in Jewish communities *

Haptoglobin and transferrin types have been determined by starch gel electrophoresis on blood from 929 subjects belonging to various Jewish communities. The frequency of the Hp 1 gene in 499 Ashkenazic Jews is 0.29 and does not differ significantly from the value of 0–26 found in 345 Jews of Oriental origin. The Hp 1 frequency of Ashkenazic Jews is significantly lower than that reported for the autochthonous populations of Central and Western Europe. Two small samples collected among Sephardic Jews and among the offspring of intercommunity marriages exhibit somewhat higher frequencies of the Hp 1 gene. The modified 2-1 phenotype was found in a single subject from Baghdad. There were three cases of ahaptoglobinaemia among Ashkenazic Jews and three among the Oriental groups. No ahaptoglobinaemia was discovered in a family sample of ninety-two Jews from Kurdistan among whom thalassaemia minor was common and the majority of whom were affeeted with G-6-P-D deficiency. All transferrins were of type C.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66130/1/j.1469-1809.1962.tb01307.x.pd

Upregulation of IFNɣ-mediated chemokines dominate the immune transcriptome of muscle-invasive urothelial carcinoma

Tumor inflammation is prognostically significant in high-grade muscle-invasive bladder cancer (MIBC). However, the underlying mechanisms remain elusive. To identify inflammation-associated immune gene expression patterns, we performed transcriptomic profiling of 40 MIBC archival tumors using the NanoString nCounter Human v.1.1 PanCancer Panel. Findings were validated using the TCGA MIBC dataset. Unsupervised and supervised clustering identified a distinctive immune-related gene expression profile for inflammation, characterized by significant upregulation of 149 genes, particularly chemokines, a subset of which also had potential prognostic utility. Some of the most enriched biological processes were lymphocyte activation and proliferation, leukocyte adhesion and migration, antigen processing and presentation and cellular response to IFN-γ. Upregulation of numerous IFN-γ-inducible chemokines, class II MHC molecules and immune checkpoint genes was detected as part of the complex immune response to MIBC. Further, B-cell markers linked to tertiary lymphoid structures were upregulated, which in turn is predictive of tumor response to immunotherapy and favorable outcome. Our findings of both an overall activated immune profıle and immunosuppressive microenvironment provide novel insights into the complex immune milieu of MIBC with inflammation and supports its clinical significance for predicting prognosis and immunotherapeutic responsiveness, which warrants further investigation. This may open novel opportunities to identify mechanisms for developing new immunotherapeutic strategies

Kallikrein-related peptidase 6 regulates epithelial-to-mesenchymal transition and serves as prognostic biomarker for head and neck squamous cell carcinoma patients

Background: Dysregulated expression of Kallikrein-related peptidase 6 (KLK6) is a common feature for many human malignancies and numerous studies evaluated KLK6 as a promising biomarker for early diagnosis or unfavorable prognosis. However, the expression of KLK6 in carcinomas derived from mucosal epithelia, including head and neck squamous cell carcinoma (HNSCC), and its mode of action has not been addressed so far. Methods: Stable clones of human mucosal tumor cell lines were generated with shRNA-mediated silencing or ectopic overexpression to characterize the impact of KLK6 on tumor relevant processes in vitro. Tissue microarrays with primary HNSCC samples from a retrospective patient cohort (n = 162) were stained by immunohistochemistry and the correlation between KLK6 staining and survival was addressed by univariate Kaplan-Meier and multivariate Cox proportional hazard model analysis. Results: KLK6 expression was detected in head and neck tumor cell lines (FaDu, Cal27 and SCC25), but not in HeLa cervix carcinoma cells. Silencing in FaDu cells and ectopic expression in HeLa cells unraveled an inhibitory function of KLK6 on tumor cell proliferation and mobility. FaDu clones with silenced KLK6 expression displayed molecular features resembling epithelial-to-mesenchymal transition, nuclear β-catenin accumulation and higher resistance against irradiation. Low KLK6 protein expression in primary tumors from oropharyngeal and laryngeal SCC patients was significantly correlated with poor progression-free (p = 0.001) and overall survival (p < 0.0005), and served as an independent risk factor for unfavorable clinical outcome. Conclusions: In summary, detection of low KLK6 expression in primary tumors represents a promising tool to stratify HNSCC patients with high risk for treatment failure. These patients might benefit from restoration of KLK6 expression or pharmacological targeting of signaling pathways implicated in EMT

In Vitro Analysis of Integrated Global High-Resolution DNA Methylation Profiling with Genomic Imbalance and Gene Expression in Osteosarcoma

Genetic and epigenetic changes contribute to deregulation of gene expression and development of human cancer. Changes in DNA methylation are key epigenetic factors regulating gene expression and genomic stability. Recent progress in microarray technologies resulted in developments of high resolution platforms for profiling of genetic, epigenetic and gene expression changes. OS is a pediatric bone tumor with characteristically high level of numerical and structural chromosomal changes. Furthermore, little is known about DNA methylation changes in OS. Our objective was to develop an integrative approach for analysis of high-resolution epigenomic, genomic, and gene expression profiles in order to identify functional epi/genomic differences between OS cell lines and normal human osteoblasts. A combination of Affymetrix Promoter Tilling Arrays for DNA methylation, Agilent array-CGH platform for genomic imbalance and Affymetrix Gene 1.0 platform for gene expression analysis was used. As a result, an integrative high-resolution approach for interrogation of genome-wide tumour-specific changes in DNA methylation was developed. This approach was used to provide the first genomic DNA methylation maps, and to identify and validate genes with aberrant DNA methylation in OS cell lines. This first integrative analysis of global cancer-related changes in DNA methylation, genomic imbalance, and gene expression has provided comprehensive evidence of the cumulative roles of epigenetic and genetic mechanisms in deregulation of gene expression networks

Rapid Analysis of Saccharomyces cerevisiae Genome Rearrangements by Multiplex Ligation–Dependent Probe Amplification

Aneuploidy and gross chromosomal rearrangements (GCRs) can lead to genetic diseases and the development of cancer. We previously demonstrated that introduction of the repetitive retrotransposon Ty912 onto a nonessential chromosome arm of Saccharomyces cerevisiae led to increased genome instability predominantly due to increased rates of formation of monocentric nonreciprocal translocations. In this study, we adapted Multiplex Ligation–dependent Probe Amplification (MLPA) to analyze a large numbers of these GCRs. Using MLPA, we found that the distribution of translocations induced by the presence of Ty912 in a wild-type strain was nonrandom and that the majority of these translocations were mediated by only six translocation targets on four different chromosomes, even though there were 254 potential Ty-related translocation targets in the S. cerevisiae genome. While the majority of Ty912-mediated translocations resulted from RAD52-dependent recombination, we observed a number of nonreciprocal translocations mediated by RAD52-independent recombination between Ty1 elements. The formation of these RAD52-independent translocations did not require the Rad51 or Rad59 homologous pairing proteins or the Rad1–Rad10 endonuclease complex that processes branched DNAs during recombination. Finally, we found that defects in ASF1-RTT109–dependent acetylation of histone H3 lysine residue 56 (H3K56) resulted in increased accumulation of both GCRs and whole-chromosome duplications, and resulted in aneuploidy that tended to occur simultaneously with GCRs. Overall, we found that MLPA is a versatile technique for the rapid analysis of GCRs and can facilitate the genetic analysis of the pathways that prevent and promote GCRs and aneuploidy