Determinação de tiroxina (T4) e Triiodotironina (T3) em felinos domésticos pelo método de eletroquimioluminescência (ECLIA).

Ministério da Educação e Universidade Federal Rural da AmazôniaAs técnicas para determinação dos hormônios da tireoide foram criadas e continuam sendo estudadas a fim de melhorar a eficiência do diagnóstico, em função disto novas metodologias de dosagem estão surgindo, como a eletroquimioluminescência (ECLIA). O objetivo deste estudo foi determinar intervalos de referência para os hormônios da tireoide (T4 e T3) através da ECLIA, em felinos domésticos da cidade de Belém, Pará, Brasil, assim como avaliar os efeitos do sexo e faixa etária sobre essas concentrações. Foram selecionados 45 gatos sadios, sendo 17 machos e 28 fêmeas, com idades entre um e 12 anos, e realizada a mensuração das concentrações séricas de tiroxina total e triiodotironina total pelo método de ECLIA. Os intervalos de referência para os hormônios da tireoide pelo ECLIA na população estudada foram 0,612 - 1,150 nmol/L para T3 e 18,53 - 33,86 nmol/L para T4, porém não houve influencia dos fatores sexo e idade sobre as concentrações desses hormônios. Foi possível determinar as concentrações dos hormônios tireoidianos pelo método da eletroquimioluminescência em gatos domésticos, assim como estabelecer uma faixa de referência, porém mais estudos são necessários para validar o método na espécie felina com ou sem doença de tireoide.The techniques for determining the thyroid hormones have been created and are being studied in order to improve the efficiency of diagnosis, according to this, new dosage methodologies are emerging, such as electrochemiluminescence (ECLIA). The aim of this study was to establish reference intervals for thyroid hormones by ECLIA in domestic cats from Belém, Pará, Brazil and to evaluate the effects of sex and age on these concentrations. It was selected 45 cats, 17 males and 28 females, aged between one and 12 years and made the measurement of serum total thyroxine (T4) and total triiodothyronine (T3) by ECLIA method. The intervals of reference for thyroid hormones in this study were 0.612 to 1.150 nmol/L for T3 and 18.53 to 33.86 nmol/L for T4, but there was no influence of sex and age factors. It was possible to determine the concentrations of thyroid hormones by the method of electrochemiluminescence in domestic cats, as well as establish a reference range, but more studies are needed to validate the method in feline species with thyroid disease

Variação sazonal dos valores de bioquímica sérica de jiboias amazônicas (Boa constrictor constrictor) mantidas em cativeiro

http://dx.doi.org/10.5007/2175-7925.2012v25n4p165 Na região Norte do Brasil, as estações do ano não são bem definidas como acontece nas regiões Sul e Sudeste, devido ao clima equatorial quente e úmido, com um período mais chuvoso, conhecido como inverno amazônico, e um período menos chuvoso, conhecido como verão amazônico. Com esta pesquisa, objetivou-se avaliar a variação dos valores bioquímicos séricos de jiboias amazônicas correlacionadas com a sazonalidade da região. Foram realizadas análises bioquímicas séricas (AST, ALT, DHL, FA, cálcio, ácido úrico, fósforo, proteína total, albumina e globulina) de 31 serpentes da espécie Boa constrictor constrictor, mantidas em cativeiro. Levando em consideração a variável sazonalidade, observou-se que oito dos dez parâmetros são maiores no inverno em comparação ao verão (proteína total, albumina, globulina, ALT, AST, FA, DHL e cálcio) sendo que os valores de ALT, AST e cálcio mostraram ter diferenças estatísticas significantes no verão e inverno amazônicos, enquanto que os demais parâmetros parecem não ser influenciados pela sazonalidade. Esta foi a primeira pesquisa, em cativeiro, analisando o perfil bioquímico sérico serpentes Boa constrictor constrictor no Estado do Pará

Evaluation of biocompatibility of the membrane of cellulose acetate in dogs with acute renal failure undergoing hemodialysis

ABSTRACT. Meneses A.M.C., Saito M.E., Moraes C.C.G., Souza N.F., Bastos R.K.G., Luz M.A., Seixas L.S., Melchert A. & Caramori J.C.T. [Evaluation of biocompatibility of the membrane of cellulose acetate in dogs with acute renal failure undergoing hemodialysis.] Avaliação da biocompatibilidade da membrana de acetato de celulose em cães com insuficiência renal aguda submetidos à hemodiálise. Revista Brasileira de Medicina Veterinária, 36(4):362-366, 2014. Instituto da saúde e Produção Animal na Amazônia, Universidade Federal Rural da Amazônia, Avenida Presidente Tancredo Neves, 2501, Montese, Belém, PA 66077-530, Brasil. E-mail: [email protected] In order to evaluate the biocompatibility of the membrane of cellulose acetate in dogs with acute renal failure (ARF), undergoing hemodialysis, were used two groups, one consisting of eight normal dogs and the other by eight dogs with ARF induced by gentamicin. Each animal underwent five hemodialysis sessions, with intervals of 24 hours between each one. A significant reduction in urea and creatinine, whereas the other biochemical values were not different between groups, as well as blood pressure, red cell count, white blood cell count and activated clotting time. High levels of TNF-α was found in sick animals, with no detection of this cytokine in normal animals

Rotavirus research in Amazon wild birds kept in captivity in the state of Pará, Brazi

ABSTRACT. Luz M.A., Bezerra D.A., Silva R.R., Guerreiro A.N., Seixas L.S., Bastos R.K.G., Mascarenhas J. D’Arc P., Moraes C.C.G., Souza N.F. & Meneses A.M.C. [Rotavirus research in Amazon wild birds kept in captivity in the state of Pará, Brazil.] Pesquisa de rotavírus em aves silvestres da região amazônica mantidas em cativeiro no estado do Pará, Brasil. Revista Brasileira de Medicina Veterinária, 36(2):167-173, 2014. Instituto da Saúde e Produção animal na Amazônia, Universidade Federal Rural da Amazônia, Avenida Presidente Tancredo Neves, 2501, Montese, Belém, PA 66077-901, Brasil. E-mail: [email protected] This study aimed to investigate rotavirus in wild birds kept in captivity at Pará State, to detect and characterize the electropherotypes groups of circulating rotaviruses and investigate A and D rotavirus groups presence in fecal specimens of these birds. Fecal samples were collected at Fazenda Paricuiã (Terra Alta / PA, Brazil), in Jardim Zoobotânico da Amazônia Bosque Rodrigues Alves, Parque Ecológico Mangal das Garças, Museu Paraense Emílio Goeldi (MPEG) and Bioparque Amazônia Crocodilo Safari in Belém/Pará/Brazil, between March 2011 and February 2012. Were collected fecal samples from 83 birds belonging to the orders: Psittaciformes (Family Psittacidae), Ciconiformes (Ardeidae and Threskiornithidae families) and Falconiformes (Family Accipitridae). Fecal suspensions were prepared from samples collected, with subsequent extraction of viral dsRNA, which was subjected to polyacrylamide gel electrophoresis (PAGE). Reverse transcription polymerase chain reaction (RT-PCR) was performed with specific primers for amplification of NSP4 gene of A rotavirus and VP6 gene of D rotavirus. All samples were negative by both EGPA and by RT-PCR, requiring, however, further studies aimed in wild birds kept in captivity to determine the role of these species in the rotavirus epidemiology

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora