BIOCHEMICAL AND FUNCTIONAL CHARACTERIZATION OF A POTENTIAL 2’, 3’-CYCLIC-NUCLEOTIDE 3’-PHOSPHODIESTERASE (CNPASE) FOUND IN TUMORIGENIC FISH RETROVIRUSES

Tumorigenic retroviruses cause seasonal cancer in fish. Many of these retroviruses contain an interesting unknown gene of cellular origin. Computational programs predict that this gene encodes a CNPase (2’,3’-Cyclic-nucleotide 3’-phosphodiesterase). In mammals, the natural function of CNPase is unknown. The purpose of the experiment is to isolate and characterize the potential viral CNPase. Utilizing a vector from zebrafish endogenous retrovirus (ZFERV), the potential CNPase genetic sequence will be isolated and analyzed. Thereafter, the potential CNPase protein will be expressed and purified, and characterization will include enzymatic activity assays, inhibition activity studies, and NMR studies. Future studies involve functional characterization of the potential CNPase, including binding and transformation studies. Potential CNPase is predicted to function as an oncogene that promotes tumorigenesis in fish. Exploring this potential CNPase may aide in the treatment of affected fish as well as provide insight into the function of this enzyme in humans

Laboratory implementation of edge illumination X-ray phase-contrast imaging with energy-resolved detectors

Edge illumination (EI) X-ray phase-contrast imaging (XPCI) has potential for applications in different fields of research, including materials science, non-destructive industrial testing, small-animal imaging, and medical imaging. One of its main advantages is the compatibility with laboratory equipment, in particular with conventional non-microfocal sources, which makes its exploitation in normal research laboratories possible. In this work, we demonstrate that the signal in laboratory implementations of EI can be correctly described with the use of the simplified geometrical optics. Besides enabling the derivation of simple expressions for the sensitivity and spatial resolution of a given EI setup, this model also highlights the EI’s achromaticity. With the aim of improving image quality, as well as to take advantage of the fact that all energies in the spectrum contribute to the image contrast, we carried out EI acquisitions using a photon-counting energy-resolved detector. The obtained results demonstrate that this approach has great potential for future laboratory implementations of EI. © (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only

PI3K Isoform-Specific Regulation of Leader and Follower Cell Function for Collective Migration and Proliferation in Response to Injury

To ensure proper wound healing it is important to elucidate the signaling cues that coordinate leader and follower cell behavior to promote collective migration and proliferation for wound healing in response to injury. Using an ex vivo post-cataract surgery wound healing model we investigated the role of class I phosphatidylinositol-3-kinase (PI3K) isoforms in this process. Our findings revealed a specific role for p110α signaling independent of Akt for promoting the collective migration and proliferation of the epithelium for wound closure. In addition, we found an important role for p110α signaling in orchestrating proper polarized cytoskeletal organization within both leader and wounded epithelial follower cells to coordinate their function for wound healing. p110α was necessary to signal the formation and persistence of vimentin rich-lamellipodia extensions by leader cells and the reorganization of actomyosin into stress fibers along the basal domains of the wounded lens epithelial follower cells for movement. Together, our study reveals a critical role for p110α in the collective migration of an epithelium in response to wounding

Xenotransplantation of microencapsulated pancreatic islets contained in a vascular prosthesis: preliminary results

Porcine and human pancreatic islets were microencapsulated in an alginate-polylysine biomembrane and put in a chamber of a new vascular prosthesis composed of an inner tubing of Dacron mesh and an outer tubing of expanded polytetrafluorethylene material. The vascular prosthesis was anastomized between the iliac artery and the contralateral vein of diabetic dogs. The recipients did not receive any immunosuppressive therapy. Function of porcine and human islets was monitored by measuring serum glucose levels and human C-peptide concentrations. After transplantation, serum glucose levels were maintained at values lower than 200 mg/dl, and C-peptide concentrations were between 0.8 and 3.2 ng/ml. Injected insulin requirements decreased by 50%-60%. Four to 8 weeks after transplantation, histologic examination showed well-preserved and functioning islets in the majority of intact microcapsules. Fibrin and inflammatory cells were not observed in the chamber. These data suggest long-term survival and function of microencapsulated pancreatic islets in the vascular prosthesis. © 1991 Springer-Verlag

A REPEATED MEASURES ANALYSIS OF THE EFFECT OF VEGETATIVE BUFFERS ON CONTAMINANT RUNOFF FROM BERMUDAGRASS TURF

A repeated measures analysis was conducted on a set of data from a multi-year study to assess the effect of vegetative buffers on the surface runoff of selected herbicides and nutrients. Multiplicative models describing the observed behavior of runoff concentration over time for buffered and non-buffered plots were fitted on a log-transformed scale using linear mixed models with PROC MIXED in PC SAS version 6.11. A spatial power covariance structure was used. Additional models for contaminant mass flow rates were fitted to evaluate the effect of buffers on total runoff mass

Bioaccessibility Tests Accurately Estimate Bioavailability Of Lead To Quail

Hazards of soil-borne lead (Pb) to wild birds may be more accurately quantified if the bioavailability of that Pb is known. To better understand the bioavailability of Pb to birds, the authors measured blood Pb concentrations in Japanese quail (Coturnix japonica) fed diets containing Pb-contaminated soils. Relative bioavailabilities were expressed by comparison with blood Pb concentrations in quail fed a Pb acetate reference diet. Diets containing soil from 5 Pb-contaminated Superfund sites had relative bioavailabilities from 33% to 63%, with a mean of approximately 50%. Treatment of 2 of the soils with phosphorus (P) significantly reduced the bioavailability of Pb. Bioaccessibility of Pb in the test soils was then measured in 6 in vitro tests and regressed on bioavailability: the relative bioavailability leaching procedure at pH 1.5, the same test conducted at pH 2.5, the Ohio State University in vitro gastrointestinal method, the urban soil bioaccessible lead test, the modified physiologically based extraction test, and the waterfowl physiologically based extraction test. All regressions had positive slopes. Based on criteria of slope and coefficient of determination, the relative bioavailability leaching procedure at pH 2.5 and Ohio State University in vitro gastrointestinal tests performed very well. Speciation by X-ray absorption spectroscopy demonstrated that, on average, most of the Pb in the sampled soils was sorbed to minerals (30%), bound to organic matter (24%), or present as Pb sulfate (18%). Additional Pb was associated with P (chloropyromorphite, hydroxypyromorphite, and tertiary Pb phosphate) and with Pb carbonates, leadhillite (a lead sulfate carbonate hydroxide), and Pb sulfide. The formation of chloropyromorphite reduced the bioavailability of Pb, and the amendment of Pb-contaminated soils with P may be a thermodynamically favored means to sequester Pb

A comparison of lysosomal enzymes expression levels in peripheral blood of mild- and severe-Alzheimer’s disease and MCI patients: implications for regenerative medicine approaches

The association of lysosomal dysfunction and neurodegeneration has been documented in several neurodegenerative diseases, including Alzheimer’s Disease (AD). Herein, we investigate the association of lysosomal enzymes with AD at different stages of progression of the disease (mild and severe) or with mild cognitive impairment (MCI). We conducted a screening of two classes of lysosomal enzymes: glycohydrolases (β-Hexosaminidase, β-Galctosidase, β-Galactosylcerebrosidase, β-Glucuronidase) and proteases (Cathepsins S, D, B, L) in peripheral blood samples (blood plasma and PBMCs) from mild AD, severe AD, MCI and healthy control subjects. We confirmed the lysosomal dysfunction in severe AD patients and added new findings enhancing the association of abnormal levels of specific lysosomal enzymes with the mild AD or severe AD, and highlighting the difference of AD from MCI. Herein, we showed for the first time the specific alteration of β-Galctosidase (Gal), β-Galactosylcerebrosidase (GALC) in MCI patients. It is notable that in above peripheral biological samples the lysosomes are more sensitive to AD cellular metabolic alteration when compared to levels of Aβ-peptide or Tau proteins, similar in both AD groups analyzed. Collectively, our findings support the role of lysosomal enzymes as potential peripheral molecules that vary with the progression of AD, and make them useful for monitoring regenerative medicine approaches for AD

Measuring Black Hole Spin in OJ287

We model the binary black hole system OJ287 as a spinning primary and a non-spinning secondary. It is assumed that the primary has an accretion disk which is impacted by the secondary at specific times. These times are identified as major outbursts in the light curve of OJ287. This identification allows an exact solution of the orbit, with very tight error limits. Nine outbursts from both the historical photographic records as well as from recent photometric measurements have been used as fixed points of the solution: 1913, 1947, 1957, 1973, 1983, 1984, 1995, 2005 and 2007 outbursts. This allows the determination of eight parameters of the orbit. Most interesting of these are the primary mass of $1.84\cdot 10^{10} M_\odot$, the secondary mass $1.46\cdot 10^{8} M_\odot$, major axis precession rate $39^\circ.1$ per period, and the eccentricity of the orbit 0.70. The dimensionless spin parameter is $0.28\:\pm\:0.01$ (1 sigma). The last parameter will be more tightly constrained in 2015 when the next outburst is due. The outburst should begin on 15 December 2015 if the spin value is in the middle of this range, on 3 January 2016 if the spin is 0.25, and on 26 November 2015 if the spin is 0.31. We have also tested the possibility that the quadrupole term in the Post Newtonian equations of motion does not exactly follow Einstein's theory: a parameter $q$ is introduced as one of the 8 parameters. Its value is within 30% (1 sigma) of the Einstein's value $q = 1$. This supports the $no-hair theorem$ of black holes within the achievable precision. We have also measured the loss of orbital energy due to gravitational waves. The loss rate is found to agree with Einstein's value with the accuracy of 2% (1 sigma).Comment: 12 pages, 4 figures, IAU26