4,084 research outputs found
Development tests for the 2.5 megawatt Mod-2 wind turbine generator
The 2.5 megawatt MOD-2 wind turbine generator test program is discussed. The development of the 2.5 megawatt MOD-2 wind turbine generator included an extensive program of testing which encompassed verification of analytical procedures, component development, and integrated system verification. The test program was to assure achievement of the thirty year design operational life of the wind turbine system as well as to minimize costly design modifications which would otherwise have been required during on site system testing. Computer codes were modified, fatigue life of structure and dynamic components were verified, mechanical and electrical component and subsystems were functionally checked and modified where necessary to meet system specifications, and measured dynamic responses of coupled systems confirmed analytical predictions
Coherent photodissociation reactions: Observation by a novel picosecond polarization technique
In this communication, we wish to report on a novel picosecond polarization method for measuring the degree of rotational coherence that is preserved in photodissociation reactions. The systems studied here are jet-cooled van der Waals molecules; stilbene [4-6] bound [5] to He or Ne with a 1:1 composition.[7
DNA unwinding heterogeneity by RecBCD results from static molecules able to equilibrate.
Single-molecule studies can overcome the complications of asynchrony and ensemble-averaging in bulk-phase measurements, provide mechanistic insights into molecular activities, and reveal interesting variations between individual molecules. The application of these techniques to the RecBCD helicase of Escherichia coli has resolved some long-standing discrepancies, and has provided otherwise unattainable mechanistic insights into its enzymatic behaviour. Enigmatically, the DNA unwinding rates of individual enzyme molecules are seen to vary considerably, but the origin of this heterogeneity remains unknown. Here we investigate the physical basis for this behaviour. Although any individual RecBCD molecule unwound DNA at a constant rate for an average of approximately 30,000 steps, we discover that transiently halting a single enzyme-DNA complex by depleting Mg(2+)-ATP could change the subsequent rates of DNA unwinding by that enzyme after reintroduction to ligand. The proportion of molecules that changed rate increased exponentially with the duration of the interruption, with a half-life of approximately 1 second, suggesting that a conformational change occurred during the time that the molecule was arrested. The velocity after pausing an individual molecule was any velocity found in the starting distribution of the ensemble. We suggest that substrate binding stabilizes the enzyme in one of many equilibrium conformational sub-states that determine the rate-limiting translocation behaviour of each RecBCD molecule. Each stabilized sub-state can persist for the duration (approximately 1 minute) of processive unwinding of a DNA molecule, comprising tens of thousands of catalytic steps, each of which is much faster than the time needed for the conformational change required to alter kinetic behaviour. This ligand-dependent stabilization of rate-defining conformational sub-states results in seemingly static molecule-to-molecule variation in RecBCD helicase activity, but in fact reflects one microstate from the equilibrium ensemble that a single molecule manifests during an individual processive translocation event
Dynamics of clusters: From elementary to biological structures
Between isolated atoms or molecules and bulk materials there lies a class of unique structures, known as clusters, that consist of a few to hundreds of atoms or molecules. Within this range of "nanophase," many physical and chemical properties of the materials evolve as a function of cluster size, and materials may exhibit novel properties due to quantum confinement effects. Understanding these phenomena is in its own rights fundamental, but clusters have the additional advantage of being controllable model systems for unraveling the complexity of condensed-phase and biological structures, not to mention their vanguard role in defining nanoscience and nanotechnology. Over the last two decades, much progress has been made, and this short overview highlights our own involvement in developing cluster dynamics, from the first experiments on elementary systems to model systems in the condensed phase, and on to biological structures
4D Lorentz Electron Microscopy Imaging: Magnetic Domain Wall Nucleation, Reversal, and Wave Velocity
Magnetization reversal is an important topic of research in the fields of both basic and applied ferromagnetism. For the study of magnetization reversal dynamics and magnetic domain wall (DW) motion in ferromagnetic thin films, imaging techniques are indispensable. Here, we report 4D imaging of DWs by the out-of-focus Fresnel method in Lorentz ultrafast electron microscopy (UEM), with in situ spatial and temporal resolutions. The temporal change in magnetization, as revealed by changes in image contrast, is clocked using an impulsive optical field to produce structural deformation of the specimen, thus modulating magnetic field components in the specimen plane. Directly visualized are DW nucleation and subsequent annihilation and oscillatory reappearance (periods of 32 and 45 ns) in nickel films on two different substrates. For the case of Ni films on a Ti/Si_(3)N_4 substrate, under conditions of minimum residual external magnetic field, the oscillation is associated with a unique traveling wave train of periodic magnetization reversal. The velocity of DW propagation in this wave train is measured to be 172 m/s with a wavelength of 7.8 μm. The success of this study demonstrates the promise of Lorentz UEM for real-space imaging of spin switching, ferromagnetic resonance, and laser-induced demagnetization in ferromagnetic nanostructures
Multiwavelength Monitoring of the Dwarf Seyfert 1 Galaxy NGC 4395. IV. The Variable UV Absorption Lines
We report the detection of variable UV absorption lines in NGC 4395, based on
UV observations with the HST STIS carried out in April and July, 2004, as part
of a reverberation-mapping campaign. Low-ionization lines of O I, N I, Si II, C
II, and Fe II, are present in the low-state spectra (April 2004) at a velocity
v_shift=-250 km/s (system A_l), and additional high-ionization lines of C IV
and N V appear in the high-state spectra (July 2004) at v_shift=-250 km/s
(system A_h) and at v_shift=-840 km/s (system B). The absence of absorption
from the low metastable levels of Si II implies a density <~10^3 cm^(-3) for
system A_l, indicating a location outside the narrow line region (NLR). System
A_h is peculiar as only N V absorption is clearly detected. A high N V/C IV
absorption ratio is expected for a high metallicity absorber, but this is
excluded here as the metallicity of the host galaxy and of the nuclear gas is
significantly subsolar. A simple acceptable model for systems A_h and B is an
absorber located between the broad line region (BLR) and the NLR, which absorbs
only the continuum and the BLR. At the low-state the strong narrow emission
lines of C IV and N V dominate the spectrum, making the absorption invisible.
At the high-state the absorbed continuum and BLR emission dominate the
spectrum. Thus, the change in the observed absorption does not reflect a change
in the absorber, but rather a change in the continuum and BLR emission from
behind the absorber, relative to the emission from the NLR in front of the
absorber. Studies of the absorption line variability in highly variable objects
can thus break the degeneracy in the absorber distance determination inherent
to single epoch studies.Comment: Accepted for publication in The Astrophysical Journa
Theory of one-dimensional double-barrier quantum pump in two-frequency signal regime
A one-dimensional system with two -like barriers or wells
bi-chromaticaly oscillating at frequencies and is
considered. The alternating signal leads to the direct current across the
structure (even in a symmetric system). The properties of this quantum pump are
studied in a wide range of the system parameters.Comment: 4 pages, 5 figure
Nonchaotic Nonlinear Motion Visualized in Complex Nanostructures by Stereographic 4D Electron Microscopy
Direct electron imaging with sufficient time resolution is a powerful tool for visualizing the three-dimensional (3D) mechanical motion and resolving the four-dimensional (4D) trajectories of many different components of a nanomachine, e.g., a NEMS device. Here, we report a nanoscale nonchaotic motion of a nano- and microstructured NiTi shape memory alloy in 4D electron microscopy. A huge amplitude oscillatory mechanical motion following laser heating is observed repetitively, likened to a 3D motion of a conductor’s baton. By time-resolved 4D stereographic reconstruction of the motion, prominent vibrational frequencies (3.0, 3.8, 6.8, and 14.5 MHz) are fully characterized, showing evidence of nonlinear behavior. Moreover, it is found that a stress (fluence)−strain (displacement) profile shows nonlinear elasticity. The observed resonances of the nanostructure are reminiscent of classical molecular quasi-periodic behavior, but here both the amplitude and frequency of the motion are visualized using ultrafast electron microscopy
Ultrafast vectorial and scalar dynamics of ionic clusters: Azobenzene solvated by oxygen
The ultrafast dynamics of clusters of trans-azobenzene anion (A–) solvated by oxygen molecules was investigated using femtosecond time-resolved photoelectron spectroscopy. The time scale for stripping off all oxygen molecules from A– was determined by monitoring in real time the transient of the A– rise, following an 800 nm excitation of A– (O2)n, where n=1–4. A careful analysis of the time-dependent photoelectron spectra strongly suggests that for n>1 a quasi-O4 core is formed and that the dissociation occurs by a bond cleavage between A– and conglomerated (O2)n rather than a stepwise evaporation of O2. With time and energy resolutions, we were able to capture the photoelectron signatures of transient species which instantaneously rise (2- for A–O2 and A·O4-·(O2)n–2 for A–(O2)n, where n=2–4. Subsequent to an ultrafast electron recombination, A– rises with two distinct time scales: a subpicosecond component reflecting a direct bond rupture of the A–-(O2)n nuclear coordinate and a slower component (1.6–36 ps, increasing with n) attributed to an indirect channel exhibiting a quasistatistical behavior. The photodetachment transients exhibit a change in the transition dipole direction as a function of time delay. Rotational dephasing occurs on a time scale of 2–3 ps, with a change in the sign of the transient anisotropy between A–O2 and the larger clusters. This behavior is a key indicator of an evolving cluster structure and is successfully modeled by calculations based on the structures and inertial motion of the parent clusters
- …