48 research outputs found

    Pattern Views: Concept and Tooling for Interconnected Pattern Languages

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    Patterns describe proven solutions for recurring problems. Typically, patterns in a particular domain are interrelated and organized in pattern languages. As real-world problems often require patterns of multiple domains, different pattern languages have to be considered to address these problems. However, cross-domain knowledge about how patterns of different languages relate to each other is either hidden in individual pattern descriptions or not documented at all. This makes it difficult to identify relevant patterns across pattern languages. Therefore, we introduce a concept and tooling that enables to capture patterns and their relations across pattern languages for a particular problem context

    Optical immunoprobe development for multiresidue monitoring in water

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    Aquifers used for drinking water production require regular monitoring for organic pollutants. Pollutant levels and pollutant patterns may change rapidly especially in surface water. Monitoring systems capable of unattended and automated operation are desirable e.g. at pumping sites. In this paper we report on a study of the application of immunoanalytical techniques for flexible and automated multiresidue testing. A solid phase fluorescence immunoassay with immobilised analyte derivate and free, fluorescence labelled antibody is used. Two optical transducers were tested: A simple 'slab'-waveguide made of sheet glass and an integrated optical (IO) waveguide. Bulk fluorophore excitation was used to estimate the performance of each transducer. Both transducers allow an antibody surface coverage of less than 1% of a monolayer of protein to be detected. The direct and covalent immobilisation of analyte derivates at the transducer surface for a binding inhibition assay approach is compared to a competitive assay with immobilisation of analyte derivates via an auxiliary antibody conjugate. The use of this auxiliary system allows the testing of different analytes at the same transducer surface. Atrazine was selected as a model analyte for the first trials. The ELISA type assay gives a test midpoint at 2.2µg/l and an estimated limit of detection of 0.3µg/l. The fluoroimmunoprobe with a binding inhibition assay has a test midpoint for atrazine at about 6µg/l. In the competitive assay with an auxiliary antibody conjugate signal levels were reduced by a factor of two and competition of free atrazine was poor. Titration with free analyte derivate (atrazine caproic acid) confirmed that this may be optimised by changing the competing derivate

    River Analyser - multiresidue immunoanalytical monitoring tools

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    Immunoanalytical techniques find growing acceptance in the field of environmental monitoring (Van Emon and Gerlach 1995). Although immunoassays have many attractive features, the lack of multiresidue approaches is a serious obstacle in environmental monitoring. In many environmental situations the simultaneous determination of more than one analyte is required. The situation is further complicated by the fact, that the analyte panels, which are to be detected, may vary with location and season. The RIANA project (RIver ANAlyser) is focused on immunoanalytical tools, that allow to detect multiple analytes in a single run. The application areas foreseen are online monitoring - e.g. at pumping sites or transportable devices for field use. In this paper we describe the concept and give first results
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