Alternative Splicing Changes Promoted by NOVA2 Upregulation in Endothelial Cells and Relevance for Gastric Cancer

Angiogenesis is crucial for cancer progression. While several anti-angiogenic drugs are in use for cancer treatment, their clinical benefits are unsatisfactory. Thus, a deeper understanding of the mechanisms sustaining cancer vessel growth is fundamental to identify novel biomarkers and therapeutic targets. Alternative splicing (AS) is an essential modifier of human proteome diversity. Nevertheless, AS contribution to tumor vasculature development is poorly known. The Neuro-Oncological Ventral Antigen 2 (NOVA2) is a critical AS regulator of angiogenesis and vascular development. NOVA2 is upregulated in tumor endothelial cells (ECs) of different cancers, thus representing a potential driver of tumor blood vessel aberrancies. Here, we identified novel AS transcripts generated upon NOVA2 upregulation in ECs, suggesting a pervasive role of NOVA2 in vascular biology. In addition, we report that NOVA2 is also upregulated in ECs of gastric cancer (GC), and its expression correlates with poor overall survival of GC patients. Finally, we found that the AS of the Rap Guanine Nucleotide Exchange Factor 6 (RapGEF6), a newly identified NOVA2 target, is altered in GC patients and associated with NOVA2 expression, tumor angiogenesis, and poor patient outcome. Our findings provide a better understanding of GC biology and suggest that AS might be exploited to identify novel biomarkers and therapeutics for anti-angiogenic GC treatments

Produção de girassol em resposta à utilização de boro e a adubação nitrogenada de cobertura.

Resumo: Um experimento foi realizado na safrinha 2013 para avaliar a produtividade e altura de planta em função das doses de boro (B) e adubação nitrogenada na cultura do girassol. A semeadura foi realizada em 25 de fevereiro de 2013 com o híbrido de girassol BRS 323, em sucessão à soja em área de histórico de plantio direto. Foram utilizados seis doses de B no plantio (0, 1, 2, 4, 8 e 16 kg ha), na forma de ácido bórico (17% B), com e sem a aplicação de 50 kg ha de N, em cobertura, na forma de ureia (45% N), aplicado 20 dias após a emergência das plantas, com 4 repetições. Não se observou efeito de doses de B para as variáveis analisadas. No entanto, o rendimento de girassol e a altura das plantas foram fortemente influenciados pela aplicação de N em cobertura. Abstract: An experiment was carried out in 2013 growing season to evaluate yield and plant height in function of doses of boron (B) and nitrogen fertilization on the sunflower crop. Sowing was performed on February 25, 2013 using the sunflower hybid BS 323, in sucession to soybean, in a non-till area. We used 6 B doses at planting (0, 1, 2, 4, 8 and 16 kg ha th) using as boric acid (17% B) and, 50 kg ha N by urea (45% N) in topdressing, applied 20 days after seedling emergence, with 4 replications. We observed no effect of doses of B for the analyzed variables. However, the sunflower yield and plant height were strongly influenced by the presence of nitrogen

Produção de girassol em resposta à utilização de fontes e doses de nitrogênio.

Resumo: O objetivo deste trabalho foi avaliar a resposta do girassol à aplicação de fontes e doses de nitrogênio (N). Foram utilizadas duas fontes de N (ureia e nitrato de amônio) e cinco doses aplicadas a lanço em cobertura, com três repetições. Na safra 2012, a semeadura foi realizada no dia 9 de março utilizando o híbrido BRS 321 e, na safra 2013, a semeadura foi realizada em 25 de fevereiro utilizando o híbrido BRS 323. A cobertura com N foi realizada aos 32 e 20 dias após a emergência das plantas em 2012 e 2013, respectivamente. Não houve diferença estatística significativa para as variáveis produtividade e altura da planta entre as fontes de N aplicadas e não houve interação significativa entre as fontes e doses, nas duas safras. No entanto, a produtividade na safra 2013 foi influenciada pelas diferentes doses de N. Abstract: The aim of this study was to evaluate the response of sunflower to the application of sources and doses of nitrogen (N). Two sources of N (urea and ammonium nitrate) and five doses were used, with three replications. In 2012 growing season, sowing was held on March 9 using the hybrid BRS 321 and, in 2013, sowing was done on February 25 using the hybrid BRS 323. Coverage with N was performed at 32 and 20 days after plant emergence in 2012 and 2013, respectively. No statistical difference was found for yield and plant height among the sources of N and there was no significant interaction between the sources and doses, in the two seasons. However, yield was influenced by doses of N during 2013 growing season

Produtividade de milho 2ª safra consorciado com espécies de braquiária e adubação nitrogenada em cobertura.

O objetivo deste trabalho foi avaliar a produtividade de grãos de milho cultivado na 2ª safra em função da consorciação com espécies de Brachiaria e aplicação de N em cobertura

Food groups, oils and butter, and cancer of the oral cavity and pharynx

To elucidate the role of dietary habits, a study was carried out in 1992-1997 in the province of Pordenone in Northeastern Italy, and those of Rome and Latina in central Italy. Cases were 512 men and 86 women with cancer of the oral cavity and pharynx (lip, salivary glands and nasopharynx excluded) and controls were 1008 men and 483 women who had been admitted to local hospitals for a broad range of acute non-neoplastic conditions. The validated dietary section of the questionnaire included 78 foods or recipes and ten questions on fat intake patterns. After allowance for education, smoking, alcohol and total energy intake, significant trends of increasing risk with increasing intake emerged for soups, eggs, processed meats, cakes and desserts, and butter. Risk was approximately halved in the highest compared to the lowest intake quintile for coffee and tea, white bread, poultry, fish, raw and cooked vegetables, citrus fruit, and olive oil. The inverse association with oils, especially olive oil, was only slightly attenuated by allowance for vegetable intake. Thus, frequent consumption of vegetables, citrus fruit, fish and vegetable oils were the major features of a low-risk diet for cancer of the oral cavity and pharynx