PURIFICATION OF EXOINULINASE BIFIDOBACTERIUM BREVE (PEMURNTAN EKSOINULINASE DARI BIFIDOBACTERIUM BREVE)

ABSTRACT Extracellular exoinulinase was produced from Bifidobacterium breve in media with inulin as the only C-source. The enzyme was purified on DEAE-Sephadex A-50, preparative IEF and gel filtration yielded a purification of 68-fold. The pI range was from 4.2 to 4.9, the molecular weight was identified using polyacrylamide gel electrophoresis (SDS-PAGE) and the result was 6459 and 32 kD. It did not hydrolyse inulin into smaller fructan units or sucrose, therefore it was characterized as an exo-type enzyme. Key words: Bifidobacterium, exoinulinase, inulin ABSTRAK Eksoinulinase ekstraseluler dihasilkan dari Bifidobacterium breve dalam media inulin sebagai satu¬satunya sumber karbonnya. Enzim dimurnikan menggunakan DEAE Sephadex A-50, IEF Preparative, filtrasi gel, dan diperoleh kemurnian 68 kali. Harga pI yang diperoleh berkisar dari 4,2 sampai 4,9 dengan massa molekul berturut-turut: 64, 59 dan 32 kD yang diuji dengan poliacrilamide gel elektroforesis (SDS-PAGE). Enzim tidak dapat menghidrolisis inulin kedalam unit-unit fruktan atau sukrosa, sehingga diperoleh suatu kesimpulan bahwa ensim tersebut dikarakterisasikan sebagai tipe eksoensim. Kata Kunci: Bifidobacterium, eksoinulinase, inuli

BPS Solitons in M5-Brane Worldvolume Theory with Constant Three-Form Field

We study BPS solutions for a self-dual string and a neutral string in M5-brane worldvolume theory with constant three-form field. We further generalize such solitons to superpose with a calibrated surface. We also study a traveling wave on a calibrated surface in the constant three-form field background.Comment: 12 pages, LaTeX, minor correction, added referenc

A Brane Teaser

In this note we study the puzzle posed by two M5-branes intersecting on a string (or equivalently, a single M5-brane wrapping a holomorphic four-cycle in C^4). It has been known for a while that this system is different from all other configurations built using self-intersecting M-branes; in particular the corresponding supergravity solution exhibits various curious features which have remained unexplained. We propose that the resolution to these puzzles lies in the existence of a non-zero two-form on the M5-brane world-volume.Comment: 21 pages. References adde

Dynamics of Weyl Scale Invariant non-BPS p=3 Branes

In this paper a Weyl scale invariant $p=3$ brane scenario is introduced, with the brane embedded in a higher dimensional bulk space with $N=1, 5D$ Super--Weyl symmetry. Its action, which describes its long wave oscillation modes into the ambient superspace and breaks the target symmetry down to the lower dimensional Weyl W(1,3) symmetry, is constructed by the approach of coset method.Comment: 12 pages, modified versio

The structure of E(10) at higher A(9) levels: a first algorithmic approach

The conjecture of a hidden E10 symmetry of M-theory is supported by the close connection between the dynamics of D = 11 supergravity near a spacelike singularity and a truncation of an one-dimensional ?-model with E10 symmetry where all representations beyond SL(10) level ell = 3 are omitted. If this conjecture is right, higher-level representations should especially capture the dynamics of further M-theory degrees of freedom. Unfortunately, the level by level determination of E10 commutators which is necessary to extend the model to higher levels is both an involved and toilsome task that requires computer aid. In this work, some of the relevant problems are exposed and algorithmic methods are developed which simplify key steps in the determination of explicit E10 commutators at higher levels. As an application, we compute the commutator of the level-two six-form with itsel