[Glucocorticoid induced TNFR-related protein (GITR) as marker of human regulatory T cells: expansion of the GITR(+)CD25⁻ cell subset in patients with systemic lupus erythematosus].

Objectives: Regulatory T cells (TREG) represent a T cell subset able to modulate immune response by suppressing autoreactive T-lymphocytes. The evidence of a reduced number and an impaired function of this cell population in autoimmune/ inflammatory chronic diseases led to the hypothesis of its involvement in the pathogenesis of these disorders. Glucocorticoid-induced TNFR-related protein (GITR) is a well known marker of murine TREG cells, but little is known in humans. The aim of this study was to investigate the characteristics of TREG cells in systemic lupus erythematosus (SLE) and the potential role of GITR as marker of human TREG. Methods: Nineteen SLE patients and 15 sex- and age-matched normal controls (NC) were enrolled. CD4+ T cells were magnetic sorted from peripheral blood by negative selection. Cell phenotype was analyzed through flow-cytometry using primary and secondary antibodies and real time polymerase-chain reaction (PCR) using TaqMan probes. Results: The CD25highGITRhigh subset was significantly decreased in SLE patients with respect to NC (0.37±0.21% vs 0.72±0.19%; p<0.05). On the opposite, the CD25-GITRhigh cell population was expanded in the peripheral blood of SLE patients (3.5±2.25 vs 0.70±0.32%, p<0.01). Interestingly, FoxP3 at mRNA level was expressed in both CD25- GITRhigh and CD25highGITRhigh cells, suggesting that both cell subsets have regulatory activity. Conclusions: CD4+CD25-GITRhigh cells are increased in SLE as compared to NC. The expression of high level of GITR, but not CD25, on FoxP3+ cells appears to point to a regulatory phenotype of this peculiar T cell subset

One year in review 2020:comorbidities, diagnosis and treatment of primary Sjogren's syndrome

Primary Sjogren's syndrome (pSS) is a complex and heterogeneous disorder characterised by a wide spectrum of glandular and extra-glandular features. The discovery of novel biomarkers allowed to characterise the disease not only phenotypically on the basis of clinical presentation, but also on the basis of the endotype. Moreover, a better stratification of patients has important value in the evaluation of mechanisms underlying the risk of lymphoproliferative disorders in these patients. Finally, novel targeted therapies may open new possibilities for the application of personalised medicine in pSS

One year in review 2020:comorbidities, diagnosis and treatment of primary Sjogren's syndrome

Primary Sjogren's syndrome (pSS) is a complex and heterogeneous disorder characterised by a wide spectrum of glandular and extra-glandular features. The discovery of novel biomarkers allowed to characterise the disease not only phenotypically on the basis of clinical presentation, but also on the basis of the endotype. Moreover, a better stratification of patients has important value in the evaluation of mechanisms underlying the risk of lymphoproliferative disorders in these patients. Finally, novel targeted therapies may open new possibilities for the application of personalised medicine in pSS

Quenched BKB_K-parameter with the Wilson and Clover actions at β=6.0\beta = 6.0

We present results for the Kaon $B$ parameter from a sample of $200$ configurations using the Wilson action and $460$ configurations using the Clover action, on a $18^3 \times 64$ lattice at $\beta=6.0$. A slight improvement of the chiral behaviour of $B_K$ is observed due to the Clover action. We have also compared the results for $B_K$ obtained from two different procedures for the boosting of the coupling constant $g$. We observe a strong dependence of $B_K$ on the prescription adopted for $g$ in the Wilson case, contrary to the results of the Clover case which are almost unaffected by the choice of $g$. Combining some recently obtained non perturbative estimates for the renormalisation constants with our Clover matrix element, we observe a significant improvement in the chiral behaviour of $B_K$.Comment: 3 pages, Latex, Postscript file with figures available at ftp://hpteo.roma1.infn.it/pub/preprints/lat94/donini ; to appear in Lattice '94, Nucl. Phys. (Proc.Suppl.

APE Results of Hadron Masses in Full QCD Simulations

We present numerical results obtained in full QCD with 2 flavors of Wilson fermions. We discuss the relation between the phase of Polyakov loops and the {\bf sea} quarks boundary conditions. We report preliminary results about the HMC autocorrelation of the hadronic masses, on a $16^3 \times 32$ lattice volume, at $\beta=5.55$ with $k_{sea}=0.1570$.Comment: 3 pages, compressed ps-file (uufiles), Contribution to Lattice 9

Decay Constants of Heavy-Light Mesons

The decay constants of the heavy-light pseudoscalar mesons are studied in a high statistics run using the Wilson action at $\beta=6.0$ and $\beta=6.2$, and the clover action at $\beta=6.0$. The systematics of $O(a)$ discretisation errors are discussed. Our best estimates of the decay constants are: $f_D$ = 218(9) MeV, $f_D/f_{Ds}$ = 1.11(1) and we obtain preliminary values for $f_B$.Comment: at the Dallas Lattice Conference, October 1993. 3 pages in a single postscript file, uuencoded form. Rome Preprint 93/98