347 research outputs found
Spinning straw into milk: Can a 95% byproduct diet support milk production?
Citation: Hulett, M., Ylioja, C. M., Wickersham, T. A., & Bradford, B. J. (2016). Spinning straw into milk: Can a 95% byproduct diet support milk production? Journal of Animal Science, 94, 187-187. doi:10.2527/msasas2016-400Agriculture is challenged with the need to support increasing human populations without additional land. One way the livestock industry has addressed this is by using human inedible feedstuffs, including industrial byproducts. Many dairy and feedlot diets incorporate 20–40% byproduct feeds, but few studies have evaluated responses of lactating dairy cattle to diets composed almost entirely of byproducts. Our objective was to evaluate such a diet in comparison to a more traditional lactation diet. The control diet was primarily composed of alfalfa hay, corn silage, corn gluten feed, and corn grain. The by-product diet included wheat straw, corn hominy, post-extraction algae residue, and corn gluten feed; in addition, 4% molasses was included to improve palatability. The control and by-product diets had similar concentrations of DM (50.6%) and CP (17.2%), whereas the byproduct diet included slightly more NDF (32.9 vs. 30.5%) and less fat (4.7 vs. 5.2%). Twelve Holstein cows (154 ± 20 DIM) were blocked by parity (primiparous vs. multiparous) and randomly assigned to treatment sequence in a crossover design. Diets were fed for 20 d, with data and sample collections over the final 3 d of each period. One cow was removed from byproduct diet after refusing to consume it, and data from this period were not included in the analysis. Data were analyzed with mixed models to assess fixed effects of diet, parity, and their interaction as well as the random effects of cow and period, and significance was declared at P < 0.05. The one selective cow notwithstanding, DMI was not affected by treatment. Milk yield of multiparous cows was decreased by the byproduct diet (38.7 vs. 42.3 ± 2.2 kg/d) but there was no treatment effect in primiparous cows (39.3 vs. 39.4 ± 2.2 kg/d). The byproduct diet decreased milk fat content (3.3 vs. 3.6 ± 0.12%) and tended to decrease protein content (2.94 vs. 2.99 ± 0.05%), and energy-corrected milk yield was decreased by 5.4 kg/d in multiparous cows and 1.5 kg/d in primiparous cows. No effects on BW or BCS were detected. Despite negative productivity responses, calculated recoveries of human-edible protein and energy in the diet were increased by approximately 50% with the byproduct diet, changing from a net loss to a net gain in human-edible energy and protein. A diet composed of 95% byproduct feeds supported milk yield of 39 kg/d and increased the efficiency of production from a human-edible input perspective
Dramatic Reduction of PrPC Level and Glycosylation in Peripheral Nerves following PrP Knock-Out from Schwann Cells Does Not Prevent Transmissible Spongiform Encephalopathy Neuroinvasion
Oral prion neuroinvasion occurs independently of PrPC expression in the gut epithelium
The early replication of certain prion strains within Peyer’s patches in the
small intestine is essential for the efficient spread of disease to the brain after oral exposure.
Our data show that orally acquired prions utilize specialized gut epithelial cells
known as M cells to enter Peyer’s patches. M cells express the cellular isoform of the
prion protein, PrPC, and this may be exploited by some pathogens as an uptake receptor
to enter Peyer’s patches. This suggested that PrPC might also mediate the uptake
and transfer of prions across the gut epithelium into Peyer’s patches in order to establish
infection. Furthermore, the expression level of PrPC in the gut epithelium could influence
the uptake of prions from the lumen of the small intestine. To test this hypothesis,
transgenic mice were created in which deficiency in PrPC was specifically restricted
to epithelial cells throughout the lining of the small intestine. Our data clearly show that
efficient prion neuroinvasion after oral exposure occurred independently of PrPC expression
in small intestinal epithelial cells. The specific absence of PrPC in the gut epithelium
did not influence the early replication of prions in Peyer’s patches or disease susceptibility.
Acute mucosal inflammation can enhance PrPC expression in the intestine, implying
the potential to enhance oral prion disease pathogenesis and susceptibility. However,
our data suggest that the magnitude of PrPC expression in the epithelium lining the
small intestine is unlikely to be an important factor which influences the risk of oral
prion disease susceptibility.
IMPORTANCE The accumulation of orally acquired prions within Peyer’s patches in the
small intestine is essential for the efficient spread of disease to the brain. Little is known
of how the prions initially establish infection within Peyer’s patches. Some gastrointestinal
pathogens utilize molecules, such as the cellular prion protein PrPC, expressed on
gut epithelial cells to enter Peyer’s patches. Acute mucosal inflammation can enhance
PrPC expression in the intestine, implying the potential to enhance oral prion disease
susceptibility. We used transgenic mice to determine whether the uptake of prions into
Peyer’s patches was dependent upon PrPC expression in the gut epithelium. We show
that orally acquired prions can establish infection in Peyer’s patches independently of
PrPC expression in gut epithelial cells. Our data suggest that the magnitude of PrPC expression
in the epithelium lining the small intestine is unlikely to be an important factor
which influences oral prion disease susceptibility
Status of SuperSpec: A Broadband, On-Chip Millimeter-Wave Spectrometer
SuperSpec is a novel on-chip spectrometer we are developing for multi-object,
moderate resolution (R = 100 - 500), large bandwidth (~1.65:1) submillimeter
and millimeter survey spectroscopy of high-redshift galaxies. The spectrometer
employs a filter bank architecture, and consists of a series of half-wave
resonators formed by lithographically-patterned superconducting transmission
lines. The signal power admitted by each resonator is detected by a lumped
element titanium nitride (TiN) kinetic inductance detector (KID) operating at
100-200 MHz. We have tested a new prototype device that is more sensitive than
previous devices, and easier to fabricate. We present a characterization of a
representative R=282 channel at f = 236 GHz, including measurements of the
spectrometer detection efficiency, the detector responsivity over a large range
of optical loading, and the full system optical efficiency. We outline future
improvements to the current system that we expect will enable construction of a
photon-noise-limited R=100 filter bank, appropriate for a line intensity
mapping experiment targeting the [CII] 158 micron transition during the Epoch
of ReionizationComment: 16 pages, 10 figures, Proceedings of the SPIE Astronomical Telescopes
+ Instrumentation 2014 Conference, Vol 9153, Millimeter, Submillimeter, and
Far-Infrared Detectors and Instrumentation for Astronomy VI
A supplement containing multiple types of gluconeogenic substrates alters intake but not productivity of heat-stressed Afshari lambs
Citation: Mahjoubi, E., Amanlou, H., Yazdi, M. H., Aghaziarati, N., Noori, G. R., Vahl, C. I., . . . Baumgard, L. H. (2016). A supplement containing multiple types of gluconeogenic substrates alters intake but not productivity of heat-stressed Afshari lambs. Journal of Animal Science, 94(6), 2497-2505. doi:10.2527/jas2015-9697Thirty-two Afshari lambs were used in a completely randomized design with a 2 x 2 factorial arrangement of treatments to evaluate a nutritional supplement designed to provide multiple gluconeogenic precursors during heat stress (HS). Lambs were housed in thermal neutral (TN) conditions and fed ad libitum for 8 d to obtain covariate data (period 1 [P1]) for the subsequent experimental period (period 2 [P2]). During P2, which lasted 9 d, half of the lambs were subjected to HS and the other 16 lambs were maintained in TN conditions but pair fed (PFTN) to the HS lambs. Half of the lambs in each thermal regime were fed (top-dressed) 100 g/d of a feed supplement designed to provide gluconeogenic precursors (8 lambs in HS [heat stress with Glukosa {HSG}] and 8 lambs in PFTN [pair-fed thermal neutral with Glukosa]) and the other lambs in both thermal regimes were fed only the basal control diet (HS without Glukosa [HSC] and pair-fed thermal neutral without Glukosa). Heat stress decreased DMI (14%) and by design there were no differences between the thermal treatments, but HSG lambs had increased DMI (7.5%; P < 0.05) compared with the HSC lambs. Compared with PFTN lambs, rectal temperature and skin temperature at the rump, shoulder, and legs of HS lambs were increased (P < 0.05) at 0700 and 1400 h. Rectal temperature at 1400 h decreased for HSG lambs (0.15 +/- 0.03 degrees C; P < 0.05) compared with HSC lambs. Despite similar DMI between thermal treatments, ADG for HS and PFTN lambs in P2 was decreased 55 and 85%, respectively, compared with lambs in P1 (P < 0.01). Although the prefeeding glucose concentration was not affected by thermal treatment or diet, HSG lambs had increased postfeeding glucose concentration compared with HSC lambs (P < 0.05). In contrast to the glucose responses, circulating insulin was influenced only by thermal treatment; HS lambs had increased insulin concentration (P < 0.01) before feeding and decreased concentration (P < 0.05) after feeding compared with PFTN lambs. Heat-stressed lambs had decreased NEFA concentration before feeding (P < 0.01) but not after feeding relative to PFTN lambs. Although this nutritional strategy did not affect ADG, the lower rectal temperature in HSG lambs indicates that dietary inclusion of a mixture of glucogenic precursors can potentially benefit animal health during HS
Effect of co-infection with a small intestine-restricted helminth pathogen on oral prion disease pathogenesis in mice
The early replication of some orally-acquired prion strains upon stromal-derived follicular dendritic cells (FDC) within the small intestinal Peyer’s patches is essential to establish host infection, and for the disease to efficiently spread to the brain. Factors that influence the early accumulation of prions in Peyer’s patches can directly influence disease pathogenesis. The host’s immune response to a gastrointestinal helminth infection can alter susceptibility to co-infection with certain pathogenic bacteria and viruses. Here we used the natural mouse small intestine-restricted helminth pathogen Heligmosomoides polygyrus to test the hypothesis that pathology specifically within the small intestine caused by a helminth co-infection would influence oral prion disease pathogenesis. When mice were co-infected with prions on d 8 after H. polygyrus infection the early accumulation of prions within Peyer’s patches was reduced and survival times significantly extended. Natural prion susceptible hosts such as sheep, deer and cattle are regularly exposed to gastrointestinal helminth parasites. Our data suggest that co-infections with small intestine-restricted helminth pathogens may be important factors that influence oral prion disease pathogenesis
MKID development for SuperSpec: an on-chip, mm-wave, filter-bank spectrometer
SuperSpec is an ultra-compact spectrometer-on-a-chip for millimeter and
submillimeter wavelength astronomy. Its very small size, wide spectral
bandwidth, and highly multiplexed readout will enable construction of powerful
multibeam spectrometers for high-redshift observations. The spectrometer
consists of a horn-coupled microstrip feedline, a bank of narrow-band
superconducting resonator filters that provide spectral selectivity, and
Kinetic Inductance Detectors (KIDs) that detect the power admitted by each
filter resonator. The design is realized using thin-film lithographic
structures on a silicon wafer. The mm-wave microstrip feedline and spectral
filters of the first prototype are designed to operate in the band from 195-310
GHz and are fabricated from niobium with at Tc of 9.2K. The KIDs are designed
to operate at hundreds of MHz and are fabricated from titanium nitride with a
Tc of 2K. Radiation incident on the horn travels along the mm-wave microstrip,
passes through the frequency-selective filter, and is finally absorbed by the
corresponding KID where it causes a measurable shift in the resonant frequency.
In this proceedings, we present the design of the KIDs employed in SuperSpec
and the results of initial laboratory testing of a prototype device. We will
also briefly describe the ongoing development of a demonstration instrument
that will consist of two 500-channel, R=700 spectrometers, one operating in the
1-mm atmospheric window and the other covering the 650 and 850 micron bands.Comment: As submitted, except that "in prep" references have been update
Effects of postpartum treatment with non-steroidal anti-inflammatory drugs on milk production and culling risk in dairy cattle
Dairy Research, 2014 is known as Dairy Day, 2014Inflammation during early lactation is common in dairy cattle, and a high degree of
inflammation during this time has recently been associated with both lower productivity
and greater risk of disease during that lactation. Early lactation treatments with two
non-steroidal anti-inflammatory drugs were compared with a placebo treatment to
evaluate effects on whole-lactation productivity and retention in the herd. Both meloxicam
and sodium salicylate increased whole-lactation milk and milk protein yields by 6
to 9%, despite being administered for only 1 or 3 days in early lactation, respectively.
In addition, meloxicam treatment tended to decrease the risk of cows leaving the herd
during the lactation. These results indicate that postpartum inflammatory signals have
long-lasting effects on lactation in dairy cattle
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