Marcadores moleculares como ferramentas para estudos de genética de plantas.

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Métodos de predição do comportamento de populações de melhoramento.

Algodão; Melhoramento genéticobitstream/CNPA/19713/1/DOC108.PD

Reassessment of exclusion zones of GM cotton in Brazil: the case of Rondônia State.

Cotton (Gossypium hirsutum) was the second crop to received commercial approval to planting GM cultivars in Brazil, in 2005. At that time, the main concern was the possibility of gene flow from GM cottons to affect in situ maintenance of others species sexually compatible ? G. barbadense and G. mustelinum. To avoid this problem, the National Biosafety Technical Commision (CTNBio) conditioned the cultivation of GM cotton to the creation of GM Cotton Exclusion Zones, where just non-GM cultivars could be planted. Cotton cultivation is an important agricultural activity in Brazil and the producers are almost unanimous: fields of GM cultivars yield more, the management is less laborious and the protection against weeds and pests is higher. For these reasons, farmers in the Rondonia, a state included into the exclusion zones, requested CTNBio to remove Rondônia from the exclusion zones. To deliberate, CTNBio asked Embrapa for information. To properly answer, an expedition was performed in 20 of the state?s 52 municipalities, and only one species was found, Gossypium barbadense. Plants were present just in higly anthropized areas, mainly in dooryards of urban and rural houses, and they were maintained as medicinal plants. Plants of G. barbadense were found in 5% to 10% of the houses, protected from gene flow by walls, houses, and trees. There was no signal of interespecifc crossing, even in municipalities were conventional Gossypium hirsutum were planted. The main risk to in situ mainatenance of G. barbadense is the loss of cultural habits resulting from the expansion of the public health care, which increases the use of chemical drugs over medicinal plants. In conclusion, gene flow is not a significant concern to the preservation of Gossypium barbadense in Rondônia, and there is no reason for the state to remain as an exclusion zones

The Copepod Acartia tonsa as live feed for fat snook (Centropomus parallelus) larvae from notochord flexion to advanced metamorphosis.

From early development until the completion of metamorphosis, fat snook (Centropomus parallelus) larvae are commonly fed the rotifers Brachionus spp. and Artemia spp. nauplii. In this study, cultivated copepods Acartia tonsa were evaluated as feed for 15-to 45-day-old larvae. Two experiments were performed using twelve 30-L tanks stocked with 3.3 fat snook larvae L-1. Their initial mean weight and length were 1.35 ± 0.01 mg (mean ± standard deviation) and 3.83 ± 0.33 mm for 15-day-old larvae and 2.79 ± 1.2 mg and 6.99 ± 0.88 mm for 31-day-old larvae. Three dietary treatments were carried out in four replicates, including Rotifer (R), Artemia (A) and/or Copepod (C). Experiment 1 included Diet RA (control), Diet RC and Diet RCA; while experiment 2 included Diet A (control), Diet C and Diet AC. The survival and growth of larvae fed the Diet RCA in experiment 1 were significantly higher than the others. In experiment 2, the inclusion of copepods in the diet did not improve survival and growth, however, larvae fed Diet C had the highest DHA/EPA ratio. We conclude that the copepod Acartia tonsa provides an important nutritional benefit to fat snook larvae undergoing metamorphosis

Aplicabilidade de marcadores SSR em estudos genéticos em Gossypium Mustelinun Miers.

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Thyroid Metastasis as the Initial Presentation of an Asymptomatic Clear Cell Renal Carcinoma

Introdução: A metastização para a glândula tiroideia de um carcinoma renal de células claras é rara e só diagnosticável após análise imunohistoquímica de uma amostra histológica. O objetivo deste artigo é reportar um caso de metastização tiroideia como primeira manifestação de um carcinoma renal de células claras. Caso Clínico: Doente do sexo masculino com um nódulo sólido no lobo esquerdo da tiróide, com 5,3 cm, suspeito de malignidade, submetido a tiroidectomia total com linfadenectomia do compartimento central. A análise histológica concluiu serem duas metástases de carcinoma renal de células claras. Após estudo dirigido ao rim, o doente foi submetido a nefrectomia radical quatro meses depois. Discussão: O interesse deste caso reside no facto de a metastização para a tiroide como primeira manifestação de carcinoma renal de células claras ser rara mas, se tratada precocemente, permite uma ‘dupla resseção cirúrgica’ que pode levar a sobrevidas aos cinco anos de 80%

Identification of sida micrantha mosaic virus as the causal agent of common mosaic in cotton in Goiás.

Detection and molecular identification of viruses are fundamental to define control strategies against viral diseases, particularly for whitefly-transmitted viruses. Cotton (Gossypium) plants showing leaf mosaic symptoms and yield reduction were observed in commercial cultivars (G. hirsutum) and in plants of the cotton germplasm collection of Embrapa maintained in the field (G. hirsutum, G. barbadense, G. mustelinum). DNA was extracted from cotton plants with symptoms of mosaic and interveinal chlorosis, and a begomovirus-specific genome was amplified with degenerated universal primers, which indicated their association with a begomovirus. This virus was identified as an isolate of sida micrantha mosaic virus (SiMMV) after the amplicon sequencing. The virus could not be transmitted by whiteflies (Bemisia tabaci MEAM1) to cotton plants when the latter were used as inoculum source under protected cultivation house, suggesting a complex interaction among viruses, plants and vectors