Estudo da estrutura física do grão do milho por meio de marcadores RFLP e RAPD.

xSuplemento. Edição dos resumos do 43º Congresso Nacional de Genética

The GREATS Hβ\beta+[OIII] Luminosity Function and Galaxy Properties at z∼8\mathbf{z\sim8}: Walking the Way of JWST

The James Webb Space Telescope will allow to spectroscopically study an unprecedented number of galaxies deep into the reionization era, notably by detecting [OIII] and H$\beta$ nebular emission lines. To efficiently prepare such observations, we photometrically select a large sample of galaxies at $z\sim8$ and study their rest-frame optical emission lines. Combining data from the GOODS Re-ionization Era wide-Area Treasury from Spitzer (GREATS) survey and from HST, we perform spectral energy distribution (SED) fitting, using synthetic SEDs from a large grid of photoionization models. The deep Spitzer/IRAC data combined with our models exploring a large parameter space enables to constrain the [OIII]+H$\beta$ fluxes and equivalent widths for our sample, as well as the average physical properties of $z\sim8$ galaxies, such as the ionizing photon production efficiency with $\log(\xi_\mathrm{ion}/\mathrm{erg}^{-1}\hspace{1mm}\mathrm{Hz})\geq25.77$. We find a relatively tight correlation between the [OIII]+H$\beta$ and UV luminosity, which we use to derive for the first time the [OIII]+H$\beta$ luminosity function (LF) at $z\sim8$. The $z\sim8$ [OIII]+H$\beta$ LF is higher at all luminosities compared to lower redshift, as opposed to the UV LF, due to an increase of the [OIII]+H$\beta$ luminosity at a given UV luminosity from $z\sim3$ to $z\sim8$. Finally, using the [OIII]+H$\beta$ LF, we make predictions for JWST/NIRSpec number counts of $z\sim8$ galaxies. We find that the current wide-area extragalactic legacy fields are too shallow to use JWST at maximal efficiency for $z\sim8$ spectroscopy even at 1hr depth and JWST pre-imaging to $\gtrsim30$ mag will be required.Comment: 13 pages, 9 figures, accepted for publication in MNRA

Genetic diversity of the common bean Phaseolus vulgaris L. determined by DNA-based molecular markers.

Weselected 28 potential common bean (Phaseolus vulgaris L.) progenitors to determine their genetic diversity using RAPD technique. They were divided into two groups based on their type of storage protein (phaseolin); 18 of them had type "S" phaseolin and the other 10 had phaseolin. DNA samples of all the cultivars were amplified in vitro with oligonucleotide primiers of random sequences. A total of 276 amplification products, 144 of them being polymorphic, were obtained with 45 different primers. Multivariate analysis of these data divided the cultivars into groups and, in addition, allowed a more precise intra and intergroup distinction

Sensitivity of Corynespora cassiicola from soybean to carbendazim and prothioconazole.

The incidence of target spot disease on soybean has increased in recent years in Brazil even with intensive use of fungicides, and fungal resistance has been reported in recent studies. The objective of this study was to determine the fungicide sensitivity to carbendazim and prothioconazole in a sample of 24 isolates of Corynespora cassiicola from soybean collected from 1996 to 2011 in the states of Paraná, Mato Grosso and São Paulo (Brazil) and Corpus Christi (Paraguay). The 50% effective concentration (EC50) values were estimated by the relative mycelium growth reduction on fungicide-amended medium with the doses of 0, 0.5, 1, 10, 100 and 1000 µg of active ingredient/ mL. For carbendazim, four highly resistant isolates (EC50 > ou = 50 µg/mL) were observed from samples collected from Mato Grosso in 2008 and from Paraná and Mato Grosso in 2011. The EC50 values for prothioconazole ranged from 0.47 µg/mL to 26.44 µg/mL (mean: 5.02 µg/mL). The results reinforce the occurrence of C. cassiicola resistance to benzimidazole in Paraná and Mato Grosso states

Uso da técnica de RAPD para avaliar a diversidade genética entre isolados do nematóide do cisto da soja.

Suplemento. Edição dos resumos do 41º Congresso Nacional de Genética, Caxambu, MG, 1995

Associação de marcadores moleculares SNP com o conteúdo de ácido linolênico em sementes de soja.

O objetivo deste trabalho foi validar a associação de marcadores moleculares do tipo ?single nucleotide polymorphism? (SNP) para os genes FAD3A, FAD3B e FAD3C com o conteúdo de ácido linolênico (18:3) em sementes de soja e analisar a influência dos parâmetros genéticos destes marcadores nesta característica. Foram genotipadas 185 progênies F2 derivadas do cruzamento entre A29 (mutante para os três genes FAD3, 1% de 18:3) e Tucunaré (genótipo selvagem, 11% de 18:3). Os marcadores moleculares para os genes FAD3A, FAD3B e FAD3C explicaram a variação do conteúdo de 18:3 nas populações segregantes F2 e F2:3. Além disso, as substituições alélicas no loco FAD3A proporcionam maiores variações no conteúdo de 18:3 que as substituições nos outros dois locos