26 research outputs found
Worldwide evolution of equine RVA: four voices, one story
In this work, we used a Bayesian approach, together with all the information that was available in databases, to study the evolution of equine RVA.Instituto de VirologĂaFil: Miño, Samuel. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Parreño, Viviana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentin
Equine Coital Exanthema: new insights on the knowledge and leading perspectives for treatment and prevention
Equine coital exanthema (ECE) is a highly contagious, venereally-transmitted mucocutaneous disease, characterized by the formation of papules, vesicles, pustules and ulcers on the external genital organs of mares and stallions, and caused by equid alphaherpesvirus 3 (EHV-3). The infection is endemic worldwide and the virus is transmitted mainly through direct contact during sexual intercourse and by contaminated instruments during reproductive maneuvers in breeding facilities. The disease does not result in systemic illness, infertility or abortion, yet it does have a negative impact on the equine industry as it forces the temporary withdrawal of affected animals with the consequent disruption of mating activities in breeding facilities. The purpose of this review is to provide up-to-date relevant information on the knowledge of EHV-3 infection and to analyze new approaches on diagnostics, treatment and prevention in the interest of minimizing the negative consequences of ECE in light of the current situation of the equine industry.Instituto de VirologĂaFil: Vissani, Aldana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa: Argentina.Fil: Vissani, Aldana. Universidad del Salvador. Escuela de Veterinaria. CĂĄtedra de Enfermedades Infecciosas; ArgentinaFil: Vissani, Aldana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Damiani, Armando Mario. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Damiani, Armando Mario. Universidad Nacional de Cuyo. Facultad de Ciencias MĂ©dicas. Instituto de Medicina y BiologĂa Experimental de Cuyo; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina.Fil: Barrandeguy, Maria Edith. Universidad del Salvador. Escuela de Veterinaria. CĂĄtedra de Enfermedades Infecciosas; Argentin
Susceptibility of equid herpesvirus 3 field isolates to antiviral compounds
Inst.de VirologĂaFil: Vissani, Aldana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa: Argentina. Universidad del Salvador. Escuela de Veterinaria. CĂĄtedra de Enfermedades Infecciosas; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa: ArgentinaFil: Thiry, Etienne. University of Liege. Faculty of Veterinary Medicine. FARAH Center. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; BĂ©lgicaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Universidad del Salvador. Escuela de Veterinaria. CĂĄtedra de Enfermedades Infecciosas; Argentin
Diagnosis and control of Equine Infectious Anemia in a horse farm located in Buenos Aires province, Argentina
Inst.de VirologĂaFil: Vissani, Aldana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Reynal O'Connor, J. Laboratorio Equino S.R.L.; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Traverso, S. Laboratorio Equino S.R.L.; ArgentinaFil: Gutierrez, G. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Alvarez, Irene. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03â100%; Îș = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.Fil: Vissani, MarĂa Aldana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Instituto Nacional de TecnologĂa Agropecuaria; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de TecnologĂa Agropecuaria; ArgentinaFil: Tsai, Y.-L.. GeneReach; Estados UnidosFil: Lee, P.-Y.A.. GeneReach; Estados UnidosFil: Shen, Y.-H.. GeneReach; Estados UnidosFil: Lee, F.-C.. GeneReach; Estados UnidosFil: Wang, H.-T.T.. GeneReach; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de TecnologĂa Agropecuaria; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Barrandeguy, MarĂa Edith. Instituto Nacional de TecnologĂa Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin
Viral infections in horses in Argentina : an overview based on laboratory results
Inst.de VirologĂaFil: Vissani, Aldana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Miño, Samuel. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Carossino, Mariano. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos. Universidad del Salvador. Escuela de Veterinaria; Argentina.Fil: Micheloud, Juan Francisco. Instituto Nacional de TecnologĂa Agropecuaria (INTA). EstaciĂłn Experimental Agropecuaria Salta. Grupo de Trabajo de PatologĂa, EpidemiologĂa e InvestigaciĂłn DiagnĂłstica. Ărea de Sanidad Animal; ArgentinaFil: Russo, S. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Departamento de Rabia y
Enfermedades de Pequeños Animales, VirologĂa Dilab-SENASA; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa: ArgentinaFil: Becerra, MarĂa Luciana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa: Argentina. Universidad del Salvador. Escuela de Veterinaria. CĂĄtedra de Enfermedades Infecciosas; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Universidad del Salvador. Escuela de Veterinaria. CĂĄtedra de Enfermedades Infecciosas; Argentin
Infectious diseases in donkeys and mules: an overview and update
The global equine population comprises a total of 114 million equids, which includes an estimate of 44 million donkeys (Equus asinus) and 11 million mules. Donkey populations have gradually expanded throughout the globe, particularly in developing countries. In spite of their relevance in several countries around the world, the infectious diseases affecting this group of equids are largely unknown; consequently, the available information regarding infectious diseases affecting working equids is limited. The scope of this article is to provide a brief update on the most relevant infectious diseases affecting donkeys and mules worldwide.Instituto de VirologĂaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Universidad del Salvador. Escuela de Veterinaria; ArgentinaFil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unido
Phylogenetic and structural analyses of equine rotaviruses : three possible geographically related lineages
The structural analyses revealed that the aa substitutions
in VP7 are in the antigenic sites and in the VP7:VP6
interaction region. In accordance, most of the aa changes in VP6 are in the top of the protein involving antigenic and VP7:VP6 interaction sites. Interestingly, the structural analyses of VP8* showed a few aa differences in the antigenic sites and, all equine P[12] shown the same conserved aa sequence in VP8* pocket, only observed in P[12] genotype. Our study suggest the existence of geographically related equine RVA lineages and,
describe the aa configuration of the VP8* pocket.Inst.de VirologĂaFil: Miño, Samuel. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Parreño, Viviana. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin
Molecular evolution of H3N8 equine influenza virus in Argentina
The aim of this work was to study the molecular
characteristics of H3N8 EIV detected in Argentina, estimate their diversification and infer the potential source of infections. Nucleotide and deduced amino acid (aa) sequences for the complete HA gene of the EIV detected in Argentina between 1985 and 2012 were analyzed along with sequences obtained from the Influenza Research database. Maximum Likelihood (ML) and Bayesian phylogenetic trees were built up. Bayesian coalescent
analyses were carried out to estimate the time of the most
recent common ancestors (tMRCA) and the dynamic population growthInst.de VirologĂaFil: Olguin Perglione, Cecilia. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; ArgentinaFil: Golemba, Marcelo D. Hospital de PediatrĂa. S.A.M.I.C. âProf. Dr. Juan P.Garrahanâ; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de TecnologĂa Agropecuaria (INTA). Instituto de VirologĂa; Argentina. Universidad del Salvador; Argentin