Worldwide evolution of equine RVA: four voices, one story

In this work, we used a Bayesian approach, together with all the information that was available in databases, to study the evolution of equine RVA.Instituto de VirologíaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentin

Equine Coital Exanthema: new insights on the knowledge and leading perspectives for treatment and prevention

Equine coital exanthema (ECE) is a highly contagious, venereally-transmitted mucocutaneous disease, characterized by the formation of papules, vesicles, pustules and ulcers on the external genital organs of mares and stallions, and caused by equid alphaherpesvirus 3 (EHV-3). The infection is endemic worldwide and the virus is transmitted mainly through direct contact during sexual intercourse and by contaminated instruments during reproductive maneuvers in breeding facilities. The disease does not result in systemic illness, infertility or abortion, yet it does have a negative impact on the equine industry as it forces the temporary withdrawal of affected animals with the consequent disruption of mating activities in breeding facilities. The purpose of this review is to provide up-to-date relevant information on the knowledge of EHV-3 infection and to analyze new approaches on diagnostics, treatment and prevention in the interest of minimizing the negative consequences of ECE in light of the current situation of the equine industry.Instituto de VirologíaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina.Fil: Vissani, Aldana. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Vissani, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Damiani, Armando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Damiani, Armando Mario. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Barrandeguy, Maria Edith. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentin

Susceptibility of equid herpesvirus 3 field isolates to antiviral compounds

Inst.de VirologíaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: ArgentinaFil: Thiry, Etienne. University of Liege. Faculty of Veterinary Medicine. FARAH Center. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentin

Diagnosis and control of Equine Infectious Anemia in a horse farm located in Buenos Aires province, Argentina

Inst.de VirologíaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Reynal O'Connor, J. Laboratorio Equino S.R.L.; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Traverso, S. Laboratorio Equino S.R.L.; ArgentinaFil: Gutierrez, G. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin

On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions

Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.Fil: Vissani, María Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Tsai, Y.-L.. GeneReach; Estados UnidosFil: Lee, P.-Y.A.. GeneReach; Estados UnidosFil: Shen, Y.-H.. GeneReach; Estados UnidosFil: Lee, F.-C.. GeneReach; Estados UnidosFil: Wang, H.-T.T.. GeneReach; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrandeguy, María Edith. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin

Viral infections in horses in Argentina : an overview based on laboratory results

Inst.de VirologíaFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Carossino, Mariano. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos. Universidad del Salvador. Escuela de Veterinaria; Argentina.Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; ArgentinaFil: Russo, S. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Departamento de Rabia y Enfermedades de Pequeños Animales, Virología Dilab-SENASA; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: ArgentinaFil: Becerra, María Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentin

Infectious diseases in donkeys and mules: an overview and update

The global equine population comprises a total of 114 million equids, which includes an estimate of 44 million donkeys (Equus asinus) and 11 million mules. Donkey populations have gradually expanded throughout the globe, particularly in developing countries. In spite of their relevance in several countries around the world, the infectious diseases affecting this group of equids are largely unknown; consequently, the available information regarding infectious diseases affecting working equids is limited. The scope of this article is to provide a brief update on the most relevant infectious diseases affecting donkeys and mules worldwide.Instituto de VirologíaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; ArgentinaFil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unido

Phylogenetic and structural analyses of equine rotaviruses : three possible geographically related lineages

The structural analyses revealed that the aa substitutions in VP7 are in the antigenic sites and in the VP7:VP6 interaction region. In accordance, most of the aa changes in VP6 are in the top of the protein involving antigenic and VP7:VP6 interaction sites. Interestingly, the structural analyses of VP8* showed a few aa differences in the antigenic sites and, all equine P[12] shown the same conserved aa sequence in VP8* pocket, only observed in P[12] genotype. Our study suggest the existence of geographically related equine RVA lineages and, describe the aa configuration of the VP8* pocket.Inst.de VirologíaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin

Molecular evolution of H3N8 equine influenza virus in Argentina

The aim of this work was to study the molecular characteristics of H3N8 EIV detected in Argentina, estimate their diversification and infer the potential source of infections. Nucleotide and deduced amino acid (aa) sequences for the complete HA gene of the EIV detected in Argentina between 1985 and 2012 were analyzed along with sequences obtained from the Influenza Research database. Maximum Likelihood (ML) and Bayesian phylogenetic trees were built up. Bayesian coalescent analyses were carried out to estimate the time of the most recent common ancestors (tMRCA) and the dynamic population growthInst.de VirologíaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Golemba, Marcelo D. Hospital de Pediatría. S.A.M.I.C. “Prof. Dr. Juan P.Garrahan”; ArgentinaFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador; Argentin