Lasting Effects of Soil Compaction on Soil Water Regime Confirmed by Geoelectrical Monitoring

Despite its importance for hydrological and ecological soil functioning, characterizing, and quantifying soil structure in the field remains a challenge. Traditional characterization of soil structure often relies on point measurements, more recently, we advanced the use of minimally invasive geophysical methods that operate at plot-field scales and provide information under natural conditions. In this study, we expand the application using geoelectrical and time-domain reflectometry (TDR) monitoring of soil water dynamics to infer impacts of compaction on soil structure and function. We developed a modeling scheme combining a new pedophysical model of soil electrical conductivity and a soil-structure-informed one-dimensional water flow and heat-transfer model. The model was used to interpret Direct Current (DC)-resistivity and TDR monitoring data in compacted soils at the Soil Structure Observatory (SSO) located in the vicinity of Zürich, Switzerland. We find that (1) soil compaction leads to a persistent decrease in soil electrical resistivity and (2) that compacted soils are typically drier than non-compacted soils during long drying events. The main decrease in electrical resistivity is attributed to decreasing macroporosity and increasing connectivity of soil aggregates due to compaction. Higher water losses in compacted soils are explained in terms of enhanced evaporation. Our work advances characterization of soil structure at the field scale with electrical methods by offering a physically based explanation of the impact of soil compaction on electrical properties and by interpreting DC-resistivity data in terms of soil water dynamics

Which fractures are imaged with Ground Penetrating Radar? Results from an experiment in the Äspö Hardrock Laboratory, Sweden

Identifying fractures in the subsurface is crucial for many geomechanical and hydrogeological applications. Here, we assess the ability of the Ground Penetrating Radar (GPR) method to image open fractures with sub-mm apertures in the context of future deep disposal of radioactive waste. GPR experiments were conducted in a tunnel located 410 m below sea level within the Äspö Hard Rock Laboratory (Sweden) using 3-D surface-based acquisitions (3.4 m × 19 m) with 160 MHz, 450 MHz and 750 MHz antennas. The nature of 17 identified GPR reflections was analyzed by means of three new boreholes (BH1-BH3; 9–9.5 m deep). Out of 21 injection and outflow tests in packed-off 1-m sections, only five provided responses above the detection threshold with the maximum transmissivity reaching 7.0 × 10−10 m2/s. Most GPR reflections are situated in these permeable regions and their characteristics agree well with core and Optical Televiewer data. A 3-D statistical fracture model deduced from fracture traces on neighboring tunnel walls show that the GPR data mainly identify fractures with dips between 0 and 25°. Since the GPR data are mostly sensitive to open fractures, we deduce that the surface GPR method can identify 80% of open sub-horizontal fractures. We also find that the scaling of GPR fractures in the range of 1–10 m2 agrees well with the statistical model distribution indicating that fracture lengths are preserved by the GPR imaging (no measurement bias). Our results suggests that surface-GPR carries the resolution needed to identify the most permeable sub-horizontal fractures even in very low-permeability formations, thereby, suggesting that surface-GPR could play an important role in geotechnical workflows, for instance, for industrial-scale siting of waste canisters below tunnel floors in nuclear waste repositories

Lasting Effects of Soil Compaction on Soil Water Regime Confirmed by Geoelectrical Monitoring

Despite its importance for hydrological and ecological soil functioning, characterizing, and quantifying soil structure in the field remains a challenge. Traditional characterization of soil structure often relies on point measurements, more recently, we advanced the use of minimally invasive geophysical methods that operate at plot-field scales and provide information under natural conditions. In this study, we expand the application using geoelectrical and time-domain reflectometry (TDR) monitoring of soil water dynamics to infer impacts of compaction on soil structure and function. We developed a modeling scheme combining a new pedophysical model of soil electrical conductivity and a soil-structure-informed one-dimensional water flow and heat-transfer model. The model was used to interpret Direct Current (DC)-resistivity and TDR monitoring data in compacted soils at the Soil Structure Observatory (SSO) located in the vicinity of Zurich, Switzerland. We find that (1) soil compaction leads to a persistent decrease in soil electrical resistivity and (2) that compacted soils are typically drier than non-compacted soils during long drying events. The main decrease in electrical resistivity is attributed to decreasing macroporosity and increasing connectivity of soil aggregates due to compaction. Higher water losses in compacted soils are explained in terms of enhanced evaporation. Our work advances characterization of soil structure at the field scale with electrical methods by offering a physically based explanation of the impact of soil compaction on electrical properties and by interpreting DC-resistivity data in terms of soil water dynamics

Rapamycin prevents experimental sclerodermatous chronic graft-versus-host disease in mice

Background: The most widely used mice model of chronic graft-versus-host disease (cGvHD) is an MHC-matched bone marrow transplantation model of sclerodermatous cGvHD. A limitation of that model is that mortality is relatively low, making difficult to study the impact of potentially therapeutic compounds. Aims: To develop a more severe model of cGVHD and to assess the impact of Rapamycin administration in that model. Results: Lethally irradiated Balb/C mice were injected with 10x106 bone marrow cells and 70x106 splenocytes from B10.D2 donor mice. Twenty-one days later, all mice developed cGvHD. For the severe model, donor B10.D2 mice were injected with 0.5x106 splenocytes from Balb/C twenty-one days before transplantation. All mice from the severe model (n=8) died a median of 32 days while 3 of 7 mice in the classical model survived beyond day 52. Mean survival was decreased in the severe model compared to the classical model (32 days versus 37 days; p=0.0185). Recipient mice in the severe group experienced higher weight loss, hair loss and skin fi brosis. Numbers of T lymphocytes (231.9 ± 151.4 versus 951 ± 532.8; p=0.0032) and CD4+ T cells (63.25 ± 41.93 versus 135.0 ± 14.39; p=0.0018) per microliter of blood at day 21 were lower in the severe group than in the classical model. Moreover, number of regulatory T cells (Tregs) was decreased in the severe model (1.250 ± 0.8864 versus 8.000 ± 6.753; p=0.0151). We then investigated whether rapamycin administration could prevent GVHD in the severe model. All (n=8) mice treated with PBS (placebo) died a median of 32 days after transplantation, while 6 of 8 mice given 1 mg/kg/day i.p. rapamycin survived beyond day 52 (p=0.0012). Number of Tregs/μl was higher at day 21 in rapamycin-treated mice than in mice given PBS (2.000±1.195 versus 1.250±0.8864; p=0.0796). Moreover, number of naïve CD4+T (10.00±4.192 versus 30.25±5.185; p= 0.0089) and effector memory T cells (EMT) (30.67±3.180 versus 67.33±7.881; p= 0.0125) were higher in rapamycin mice. Finally, proliferation of EMT (assessed by fl ow cytometry using Ki-67) was higher in PBS than in rapamycin mice (45.28%±4.084 versus 31.90%± 2.003; p=0.0474). Conclusion: We have developed a mice model of severe cGVHD. Interestingly, rapamycin prevented death from cGVHD in that model, perhaps through in vivo expansion of Treg

Estimation of Fracture Compliance From Attenuation and Velocity Analysis of Full‐Waveform Sonic Log Data

In fractured rocks, the amplitudes of propagating seismic waves decay due to various mechanisms, such as geometrical spreading, solid friction, displacement of pore fluid relative to the solid frame, and transmission losses due to energy conversion to reflected and transmitted waves at the fracture interfaces. In this work, we characterize the mechanical properties of individual fractures from P wave velocity changes and transmission losses inferred from static full‐waveform sonic log data. The methodology is validated using synthetic full‐waveform sonic logs and applied to data acquired in a borehole penetrating multiple fractures embedded in a granodioritic rock. To extract the transmission losses from attenuation estimates, we remove the contributions associated with other loss mechanisms. The geometrical spreading correction is inferred from a joint analysis of numerical simulations that emulate the borehole environment and the redundancy of attenuation contributions other than geometrical spreading in multiple acquisitions with different source‐receiver spacing configurations. The intrinsic background attenuation is estimated from measurements acquired in the intact zones. In the fractured zones, the variations with respect to the background attenuation are attributed to transmission losses. Once we have estimated the transmission losses associated with a given fracture, we compute the transmission coefficient, which, on the basis of the linear slip theory, can then be related to the mechanical normal compliance of the fracture. Our results indicate that the estimated mechanical normal compliance ranges from 1 × 10−13 to 1 × 10−12 m/Pa, which, for the size of the considered fractures, is consistent with the experimental evidence available.Fil: Barbosa, Nicolás D.. Universite de Lausanne; Suiza. Universidad de Ginebra; SuizaFil: Caspari, Eva. Universite de Lausanne; SuizaFil: Rubino, Jorge German. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro. Archivo Histórico del Centro Atómico Bariloche e Instituto Balseiro | Universidad Nacional de Cuyo. Instituto Balseiro. Archivo Histórico del Centro Atómico Bariloche e Instituto Balseiro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Greenwood, Andrew. Universite de Lausanne; SuizaFil: Baron, Ludovic. Universite de Lausanne; SuizaFil: Holliger, Klaus. Universite de Lausanne; Suiza. Zhejiang University; Chin

Report on an open dataset to constrain the Balmuccia peridotite body (Ivrea-Verbano Zone, Italy) through a participative gravity-modelling challenge

The Balmuccia peridotite exposes relatively fresh mantle rocks at the Earth’s surface, and as such it is of interest for geologists and geophysicists. The outcrop is a kilometre-scale feature, yet its extent at depth is insufficiently imaged. Our aim is to provide new constraints on the shape of the density anomaly this body represents, through 3D gravity modelling. In an effort to avoid personal or methodology bias, we hereby launch an invitation and call for participative modelling. We openly provide all the necessary input data: pre-processed gravity data, geological map, in situ rock densities, and digital elevation model. The expected inversion results will be compared and jointly analysed with all participants. This approach should allow us to conclude on the shape of the Balmuccia peridotite body and the associated uncertainty. This crowd effort will contribute to the site surveys preparing a scientific borehole in the area in frame of project DIVE

Comparison of Mesenchymal Stromal Cells From Different Origins for the Treatment of Graft-vs.-Host-Disease in a Humanized Mouse Model

Mesenchymal stromal cells (MSCs) have potent immunomodulatory properties that make them an attractive tool against graft- vs.-host disease (GVHD). However, despite promising results in phase I/II studies, bone marrow (BM-) derived MSCs failed to demonstrate their superiority over placebo in the sole phase III trial reported thus far. MSCs from different tissue origins display different characteristics, but their therapeutic benefits have never been directly compared in GVHD. Here, we compared the impact of BM-, umbilical cord (UC-), and adipose-tissue (AT-) derived MSCs on T-cell function in vitro and assessed their efficacy for the treatment of GVHD induced by injection of human peripheral blood mononuclear cells in NOD-scid IL-2Rγnull HLA-A2/HHD mice. In vitro, resting BM- and AT-MSCs were more potent than UC-MSCs to inhibit lymphocyte proliferation, whereas UC- and AT-MSCs induced a higher regulatory T-cell (CD4+CD25+FoxP3+)/T helper 17 ratio. Interestingly, AT-MSCs and UC-MSCs activated the coagulation pathway at a higher level than BM-MSCs. In vivo, AT-MSC infusions were complicated by sudden death in 4 of 16 animals, precluding an analysis of their efficacy. Intravenous MSC infusions (UC- or BM- combined) failed to significantly increase overall survival (OS) in an analysis combining data from 80 mice (hazard ratio [HR] = 0.59, 95% confidence interval [CI] 0.32–1.08, P = 0.087). In a sensitivity analysis we also compared OS in control vs. each MSC group separately. The results for the BM-MSC vs. control comparison was HR = 0.63 (95% CI 0.30–1.34, P = 0.24) while the figures for the UC-MSC vs. control comparison was HR = 0.56 (95% CI 0.28–1.10, P = 0.09). Altogether, these results suggest that MSCs from various origins have different effects on immune cells in vitro and in vivo. However, none significantly prevented death from GVHD. Finally, our data suggest that the safety profile of AT-MSC and UC-MSC need to be closely monitored given their pro-coagulant activities in vitro

A Prominent Role for DC-SIGN+ Dendritic Cells in Initiation and Dissemination of Measles Virus Infection in Non-Human Primates

Measles virus (MV) is a highly contagious virus that is transmitted by aerosols. During systemic infection, CD150+T and B lymphocytes in blood and lymphoid tissues are the main cells infected by pathogenic MV. However, it is unclear which cell types are the primary targets for MV in the lungs and how the virus reaches the lymphoid tissues. In vitro studies have shown that dendritic cell (DC) C-type lectin DC-SIGN captures MV, leading to infection of DCs as well as transmission to lymphocytes. However, evidence of DC-SIGN-mediated transmission in vivo has not been established. Here we identified DC-SIGNhiDCs as first target cells in vivo and demonstrate that macaque DC-SIGN functions as an attachment receptor for MV. Notably, DC-SIGNhicells from macaque broncho-alveolar lavage and lymph nodes transmit MV to B lymphocytes, providing in vivo support for an important role for DCs in both initiation and dissemination of MV infection

Particulate matter exposure during pregnancy is associated with birth weight, but not gestational age, 1962-1992: a cohort study

<p>Abstract</p> <p>Background</p> <p>Exposure to air pollutants is suggested to adversely affect fetal growth, but the evidence remains inconsistent in relation to specific outcomes and exposure windows.</p> <p>Methods</p> <p>Using birth records from the two major maternity hospitals in Newcastle upon Tyne in northern England between 1961 and 1992, we constructed a database of all births to mothers resident within the city. Weekly black smoke exposure levels from routine data recorded at 20 air pollution monitoring stations were obtained and individual exposures were estimated via a two-stage modeling strategy, incorporating temporally and spatially varying covariates. Regression analyses, including 88,679 births, assessed potential associations between exposure to black smoke and birth weight, gestational age and birth weight standardized for gestational age and sex.</p> <p>Results</p> <p>Significant associations were seen between black smoke and both standardized and unstandardized birth weight, but not for gestational age when adjusted for potential confounders. Not all associations were linear. For an increase in whole pregnancy black smoke exposure, from the 1^st(7.4 μg/m³) to the 25^th(17.2 μg/m³), 50^th(33.8 μg/m³), 75^th(108.3 μg/m³), and 90^th(180.8 μg/m³) percentiles, the adjusted estimated decreases in birth weight were 33 g (SE 1.05), 62 g (1.63), 98 g (2.26) and 109 g (2.44) respectively. A significant interaction was observed between socio-economic deprivation and black smoke on both standardized and unstandardized birth weight with increasing effects of black smoke in reducing birth weight seen with increasing socio-economic disadvantage.</p> <p>Conclusions</p> <p>The findings of this study progress the hypothesis that the association between black smoke and birth weight may be mediated through intrauterine growth restriction. The associations between black smoke and birth weight were of the same order of magnitude as those reported for passive smoking. These findings add to the growing evidence of the harmful effects of air pollution on birth outcomes.</p