Land Grant Application- Barnes, Joseph (Lubec)

Land grant application submitted to the Maine Land Office on behalf of Joseph Barnes for service in the Revolutionary War, by their widow Lydia.https://digitalmaine.com/revolutionary_war_me_land_office/1063/thumbnail.jp

Inventory and Monitoring of Aquatic Bird Species on Lakes Mead and Mohave 2004 - 2006

This project was completed as part of a task agreements awarded by the National Park Service (NPS), Lake Mead National Recreation Area (LAME), to the Public Lands Institute (PLI) at the University of Nevada, Las Vegas (UNLV). This final report was completed under a task agreement ending on September 30, 2006, and was completed in support of an NPS project funded by the Southern Nevada Public Lands Management Act (PLMA) to inventory and monitor shoreline and near-shore natural resources on Lakes Mead and Mohave (DOI 2005). This report details inventory and monitoring efforts on shoreline and aquatic bird species between March 2004 and August 2006. Major efforts completed on this project included: Intensive monitoring of 7 sites during 184 surveys on Lakes Mead and Mohave Inventory of an additional 5 sites on these lakes during 15 surveys A total of 93 aquatic or shoreline bird species documented within LAME; 88 on Lake Mead and 45 on Lake Mohave A total of 67,670 individual aquatic birds and raptors tallied; 58,032 on Lake Mead and 9,638 on Lake Mohav

Alien Registration- Barnes, Joseph (Topsham, Sagadahoc County)

https://digitalmaine.com/alien_docs/9595/thumbnail.jp

EFFECTS OF NORTH AMERICAN GINSENG ON SEXUAL BEHAVIOR IN MALE RATS

Two North American ginseng extracts were used to test sexual behavior in healthy rats and pre-diabetic rats, with erectile dysfunction, after four weeks of oral administration. Behavioral tests assessed sexual performance while animal tissues were used to examine eNOS and nNOS protein and mRNA in central and peripheral sites known to control erectile function. Results show an improvement in erectile function during copulation in healthy rats with chronic treatment of ethanol extract of NA ginseng but not aqueous extract. In addition, eNOS and nNOS mRNA was increased in penile tissue and nNOS staining was increased in the paraventricular nucleus of the hypothalamus with acute treatment of ethanol extract. For pre-diabetic rats, ethanol extract improved erectile function during mating and penile reflex tests. Also, nNOS was increased in penile tissue with chronic treatment and eNOS with acute treatment. The results show NA ginseng ethanol extract can improve erectile function

Severe Hyperosmolar Metabolic Acidosis Due to a Large Dose of Intravenous Lorazepam

This is the publisher's version, also available electronically from http://www.nejm.org/doi/full/10.1056/NEJM200204183461618.No abstract is available for this item

Determination Of Ricin Content In Castor (Ricinus Communis L.) Tissues And Comparison Of Detoxification Methods

Experiments were conducted to test for ricin content in tissue samples from four castor cultivars, developing castor seed, germinating castor seedlings, and chemically and heat treated seed meal. Ricin content of each sample was examined via Western blotting with ricin A-chain specific antibodies. Results indicate that ricin is present solely within castor endosperm and is not present any other tissues. Samples from developing seed and germinating seedlings indicate ricin production begins around day 28 post pollination, and ricin is absent from the seedling 6 days after the onset of radicle emergence. This would seem to indicate that the purpose of ricin is to protect the seed and not the entire plant. Ricin content of seed meal treated separately with heat and chemicals was tested. It was found that hot-pressing of the seed was sufficient to denature ricin in the seed meal

An Approach to Genetic Silencing of Ricin in Castor (Ricinus Communis L.)

Castor (Ricinus communis L.) is a high-yielding oilseed crop native to tropical Africa. The seed contains ~60% oil by weight, yielding approximately 1,200 kg of oil per hectare. The oil is composed of ~90% ricinoleic acid, a unique hydroxylatty acid. Its unique composition provides castor oil with distinctive characteristics important for industrial use. Unfortunately, this valuable oilseed has not been widely cultivated in the United States since 1972, due in part to the presence of ricin in the seed. Ricin is a highly toxic lectin found in the endosperm of mature castor seed. This project sought to silence ricin production through the introduction of an RNAi element into the castor genome. The RNAi vector (pC1-RKO) containing a segment of ricin mRNA and its inverted repeat separated by a chalcone synthase A intron from pFGC5941 enclosed in a pCambia1301 backbone was created, verified via sequencing, and transformed into Agrobacterium tumefaciens for castor transformation. Fungal contamination was a serious concern; successful disinfestation used a 10-minute wash with 0.1% mercuric chloride (w/v). Media supplemented with 6-benzylaminopurine generated healthier shoots from embryo axes dissected from mature seed compared to thidiazuron-treated mesocotyls dissected from mature seed. Short treatments of thidiazuron on 6-benzylaminopurine initiated shoot cultures showed greater shoot proliferation on embryo axes dissected from mature seed. Rooting occurred with incubation on half-strength medium containing naphthaleneacetic acid or indole-3-butyric acid; however, naphthaleneacetic acid produced hardier roots which better survived acclimatization. Inoculation of embryo axis explants after 2 days pre-culture improved survivability. Likewise, transformations using A. tumefaciens cultures of 0.5 O.D.600 and lower did not lead to downstream bacterial contamination. The pCambia1304 vector was used as a test plasmid for refinement of the transformation protocol. Of the 870 pCambia1304 inoculation explants, 2 survived hygromycin screening and showed gusA activity. Of the 2,500 pC1-RKO inoculated explants, 6 survived hygromycin selection and rooted. Further analysis via PCR, end-point RT-PCR, and Western and dot-blotting showed these to be non-transformed and ricin content unaffected

Co-Localization of Basal and Proliferative Cells in the Murine Main Olfactory Epithelium and Vomeronasal Organ after Injury with Cyclophosphamide

ABSTRACT In humans, advanced malignancies are often targeted with broad-spectrum cytotoxic drugs that engender several detrimental side effects, in addition to their primary usage for eradicating cancerous cells. One of the lesser-researched of these effects, histological distortion of the olfactory system impedes a patient\u27s ability to smell, perceive flavor, and ultimately may interfere with their nutritional intake and recovery from chemotherapy. Recent studies have indicated that cytotoxic drugs can damage gustatory epithelia immediately following administration (Mukherjee & Delay, 2011, 2013). We sought to observe the histological effects that cyclophosphamide (CYP), one of the oldest and most popular alkylating antineoplastic agents, may have on the murine main olfactory epithelium (MOE) and vomeronasal organ (VNO). We utilized two immunohistochemical antibodies to label cells in the olfactory epithelia: anti-Ki67, a marker strictly associated with cell proliferation; and, anti-Keratin 5, a marker for the cytoskeleton of horizontal basal cells. Twenty-eight C57BL/6 mice were administered a single intraperitoneal injection of CYP (75 mg/kg), while 20 control mice were administered saline, all at approximately seven weeks of age. Mice were euthanized at days one, two, six, 14, 30, and 45 post injection; subsequently, they were perfused with 4% paraformaldehyde, decalcified, cryoprotected, cryosectioned, and incubated with anti-Ki67 and anti-Keratin 5 antibodies, sequentially. Quantification results by fluorescent imaging of labeled sections revealed a significant decrease in the number of proliferative cells in the MOE and VNO of CYP-injected mice within the first 10 days post injection, followed by a compensatory period of increased cell proliferation through day 45 post injection, compared to saline-injected mice. Co-localization of horizontal basal cells and proliferative cells in the MOE and VNO of CYP-injected mice was significantly amplified at approximately 14 and 45 days post injection, respectively, compared to saline-injected mice. Our results suggest that administration of CYP can rapidly depress the populations of proliferative cells in the murine MOE and VNO; consequently, horizontal basal cells may afford restoration of the proliferative cell populations in the murine MOE and VNO, 14 to 45 days post injection, respectively

An Approach to Genetic Silencing of Ricin in Castor (Ricinus Communis L.)

Castor (Ricinus communis L.) is a high-yielding oilseed crop native to tropical Africa. The seed contains ~60% oil by weight, yielding approximately 1,200 kg of oil per hectare. The oil is composed of ~90% ricinoleic acid, a unique hydroxylatty acid. Its unique composition provides castor oil with distinctive characteristics important for industrial use. Unfortunately, this valuable oilseed has not been widely cultivated in the United States since 1972, due in part to the presence of ricin in the seed. Ricin is a highly toxic lectin found in the endosperm of mature castor seed. This project sought to silence ricin production through the introduction of an RNAi element into the castor genome. The RNAi vector (pC1-RKO) containing a segment of ricin mRNA and its inverted repeat separated by a chalcone synthase A intron from pFGC5941 enclosed in a pCambia1301 backbone was created, verified via sequencing, and transformed into Agrobacterium tumefaciens for castor transformation. Fungal contamination was a serious concern; successful disinfestation used a 10-minute wash with 0.1% mercuric chloride (w/v). Media supplemented with 6-benzylaminopurine generated healthier shoots from embryo axes dissected from mature seed compared to thidiazuron-treated mesocotyls dissected from mature seed. Short treatments of thidiazuron on 6-benzylaminopurine initiated shoot cultures showed greater shoot proliferation on embryo axes dissected from mature seed. Rooting occurred with incubation on half-strength medium containing naphthaleneacetic acid or indole-3-butyric acid; however, naphthaleneacetic acid produced hardier roots which better survived acclimatization. Inoculation of embryo axis explants after 2 days pre-culture improved survivability. Likewise, transformations using A. tumefaciens cultures of 0.5 O.D.600 and lower did not lead to downstream bacterial contamination. The pCambia1304 vector was used as a test plasmid for refinement of the transformation protocol. Of the 870 pCambia1304 inoculation explants, 2 survived hygromycin screening and showed gusA activity. Of the 2,500 pC1-RKO inoculated explants, 6 survived hygromycin selection and rooted. Further analysis via PCR, end-point RT-PCR, and Western and dot-blotting showed these to be non-transformed and ricin content unaffected

COST-EFFECTIVENESS OF INTRAVENOUS NICARDIPINE VERSUS SODIUM NITROPRUSSIDE FOR POSTOPERATIVE HYPERTENSION AFTER CARDIAC SURGERY.

Postoperative hypertension after cardiac surgery is common and associated with substantial morbidity. Both sodium nitroprusside (SNP) and nicardipine (NIC) are effective in its management. SNP is inexpensive, but associated with labile blood pressure (BP) control, cardiac ischemia, and metabolite toxicity. NIC is well tolerated and provides stable BP control, but is limited by high acquisition cost. We conducted a cost-effectiveness analysis from an institutional perspective of NIC versus SNP in subjects experiencing postoperative hypertension after cardiac surgery. A retrospective, cohort study identified subjects who underwent coronary artery bypass grafting (CABG) and/or valve surgery at our institution between 2007-2009. We included adults experiencing postoperative hypertension requiring ≥ 30 minutes of either NIC or SNP infusions. Institutional-specific data from the Society of Thoracic Surgeons and University HealthSystem Consortium national databases and our financial and electronic medical records were used. The number of infusion rate changes divided by the infusion duration was calculated. We considered ≥ 1 dose change/hour to represent excessive dose changes and presumably uncontrolled blood pressure. The rate per 100 subjects in each group who avoided excessive dose changes served as the efficacy variable for the economic model. Direct postoperative costs were calculated. Data were compared with t, Wilcoxon Rank Sum, Chi-square, or Fisher's exact tests as appropriate. Log-binomial regression was used to control for surgery type and severity of illness. Of the 112 subjects identified, 72 received NIC and 40 SNP. Demographics including hypertension history, number of preoperative antihypertensive agents, surgery type, and postoperative length of stay were not significantly different. NIC required significantly fewer dose changes/hour (1.2±1.6) versus SNP (1.7±1.8, p=0.004). After controlling for surgery type and severity of illness, the risk of excessive dose changes was 60% higher in those subjects prescribed SNP compared to those prescribed NIC (adjusted relative risk = 1.60, 95%CI, 1.10-2.34, p=0.0147). In the entire cohort and each specific surgery type, NIC remained cost-effective when compared to SNP. NIC use may be limited due to decisions based solely upon acquisition costs. We found that NIC resulted in less frequent dose changes and was cost-effective when compared to SNP in the treatment of post-cardiac surgery hypertension