Stress and other factors and their effect on skeletal muscle growth and metabolism; Strengths-based lab groups improve learning of undergraduate anatomy and physiology concepts

Muscle growth is an important consideration in livestock production that affects producer profits. Producers can maximize muscle growth in animals by decreasing stress and using supplements that increase growth and efficiency. In these studies, we sought to show how stress and growth-stimulating factors affect muscle metabolism in sheep and myoblast function in cattle. First, lambs were fed diets containing no supplement, ractopamine HCl (β1 agonist), or zilpaterol HCl (β2 agonist) for 21 d and housed under thermoneutral or heat stress (40°C, 35% RH) conditions (2x3 factorial). At harvest, skeletal muscle strips were used to measure ex vivo glucose oxidation under basal and insulin-stimulated conditions. Surprisingly, we did not observe interactions among environmental treatment, dietary supplement, and incubation media for glucose oxidation rates. However, heat stress decreased (P ≤ 0.05) muscle glucose oxidation by ~21%, supplementation of β2 agonist increased (P ≤ 0.05) muscle glucose oxidation by ~15%, and addition of insulin to media increased (P 0.05) glucose oxidation by ~25%. Interestingly, supplementation of β1 agonist had no effects on muscle glucose oxidation. In our second study, myoblasts were isolated from cattle with high (/ml; High A4) or control (\u3c 20ng/ml) follicular fluid androstenedione concentrations. High A4 cows consistently wean heavier calves and thus we hypothesized that they have increased myoblast function which might be passed on to their calves. Primary myoblasts were assessed for proliferative capacity (2-h EdU pulse) in growth media (20% FBS) containing no additive (basal), TNFα, or testosterone. Percentages of myogenin-positive and desmin-positive myoblasts were determined after 4-d incubation in differentiation media (2% FBS) containing no additive (basal) or TNFα. No interactions between A4 classification and incubation conditions were found for any output. Myoblasts from High A4 cows exhibited ~9% slower (P ≤ 0.05) proliferation rates but ~25% greater (P ≤ 0.05) percentages of myogenin-positive nuclei and ~15% greater (P ≤ 0.05) percentages of desmin-positive nuclei after 4-d differentiation. These data show that physiological conditions responsible for high levels of A4 in follicular fluid may also be directing skeletal myoblasts to prematurely exit the cell cycle and begin precocious differentiation. Together these studies further demonstrate that stress has a negative impact on skeletal muscle growth, but β2 adrenergic agonists improve muscle growth and that altered androstenedione concentrations also impede proper myoblast function effectively limiting muscle growth. A final study examined how strengths-based groups affected learning in an undergraduate anatomy and physiology laboratory course. This project aimed to determine if these groups would improve grades and to assess student perceptions about learning and group work. Utilizing the CliftonStrengths for Students assessment students were classified into one of four domains based on their top identified strengths: executing, influencing, strategic thinking or relationship building. Balanced groups (n=9) contained one randomly-selected student from each of the four domains. Unbalanced groups(n=13) contained four students from the same domain. At the conclusion of the semester students completed a final practical exam and a survey of their perceptions on learning and their assigned group. Balanced groups scored greater (P\u3c0.05) on the final practical exam and overall lab scores. However, student perceptions did not differ between the groups and was generally negative. Students in the strategic thinking domain scored highest (P\u3c0.05) whereas relationship builders scored lowest (P=0.05) on the final practical exam as well as the overall laboratory grade. These findings indicate that balancing student strengths in lab groups improves learning outcomes and we believe this simple approach could be applied to in-class group work across many STEM disciplines. Advisors: Dustin T. Yates and Jessica L. Peterse

The Radial Orbit Instability in Collisionless N-Body Simulations

Using a suite of self-gravitating, collisionless N-body models, we systematically explore a parameter space relevant to the onset and behavior of the radial orbit instability (ROI), whose strength is measured by the systemic axis ratios of the models. We show that a combination of two initial conditions, namely the velocity anisotropy and the virial ratio, determines whether a system will undergo ROI and exactly how triaxial the system will become. A third initial condition, the radial shape of the density profile, plays a smaller, but noticeable role. Regarding the dynamical development of the ROI, the instability a) begins after systems collapse to their most compact configuration and b) evolves fastest when a majority of the particles have radially anisotropic orbits while there is a lack of centrally-concentrated isotropic orbits. We argue that this is further evidence that self-reinforcing torques are the key to the onset of the ROI. Our findings support the idea that a separate orbit instability plays a role in halting the ROI.Comment: accepted for publication in ApJ. 9 figures in emulateapj styl

Sodium-bearing Waste Treatment Technology Evaluation Report

Sodium-bearing waste (SBW) disposition is one of the U.S. Department of Energy (DOE) Idaho Operation Office’s (NE-ID) and State of Idaho’s top priorities at the Idaho National Engineering and Environmental Laboratory (INEEL). The INEEL has been working over the past several years to identify a treatment technology that meets NE-ID and regulatory treatment requirements, including consideration of stakeholder input. Many studies, including the High-Level Waste and Facilities Disposition Environmental Impact Statement (EIS), have resulted in the identification of five treatment alternatives that form a short list of perhaps the most appropriate technologies for the DOE to select from. The alternatives are (a) calcination with maximum achievable control technology (MACT) upgrade, (b) steam reforming, (c) cesium ion exchange (CsIX) with immobilization, (d) direct evaporation, and (e) vitrification. Each alternative has undergone some degree of applied technical development and preliminary process design over the past four years. This report presents a summary of the applied technology and process design activities performed through February 2004. The SBW issue and the five alternatives are described in Sections 2 and 3, respectively. Details of preliminary process design activities for three of the alternatives (steam reforming, CsIX, and direct evaporation) are presented in three appendices. A recent feasibility study provides the details for calcination. There have been no recent activities performed with regard to vitrification; that section summarizes and references previous work

Impaired muscle stem cell function in cows with high concentrations of androstenedione in their follicular fluid

It is unclear whether androstenedione (A4) increases muscle mass and strength similar to testosterone or whether it produces primarily catabolic effects on muscle-like estrogen (Rasmussen et al., 2000). Summers et al. (2014) observed two populations of cows that exhibit either high (\u3e40 ng/mL; High A4) or low (\u3c20 ng/mL; Low A4) concentrations of A4 within the fluid of the dominant follicle just prior to ovulation. High A4 cows had decreased reproductive rates and shorter times before falling out of the herd, but those that did produce calves weaned them ~10-kg heavier than their low A4 counterparts (Summers et al., 2014). It appears that the difference in weights is due to faster growing and more efficient skeletal muscle. High A4 cows share many characteristics with women suffering from polycystic ovary syndrome (PCOS), whose high levels of circulating androgens are associated with changes in body composition (Kirchengast and Huber, 2001)

Recurrent delirium over 12 months predicts dementia: results of the Delirium and Cognitive Impact in Dementia (DECIDE) study

Background: Delirium is common, distressing and associated with poor outcomes. Previous studies investigating the impact of delirium on cognitive outcomes have been limited by incomplete ascertainment of baseline cognition or lack of prospective delirium assessments. This study quantified the association between delirium and cognitive function over time by prospectively ascertaining delirium in a cohort aged ≥ 65 years in whom baseline cognition had previously been established. Methods: For 12 months, we assessed participants from the Cognitive Function and Ageing Study II-Newcastle for delirium daily during hospital admissions. At 1-year, we assessed cognitive decline and dementia in those with and without delirium. We evaluated the effect of delirium (including its duration and number of episodes) on cognitive function over time, independently of baseline cognition and illness severity. Results: Eighty two of 205 participants recruited developed delirium in hospital (40%). One-year outcome data were available for 173 participants: 18 had a new dementia diagnosis, 38 had died. Delirium was associated with cognitive decline (−1.8 Mini-Mental State Examination points [95% CI –3.5 to –0.2]) and an increased risk of new dementia diagnosis at follow up (OR 8.8 [95% CI 1.9–41.4]). More than one episode and more days with delirium (>5 days) were associated with worse cognitive outcomes. Conclusions: Delirium increases risk of future cognitive decline and dementia, independent of illness severity and baseline cognition, with more episodes associated with worse cognitive outcomes. Given that delirium has been shown to be preventable in some cases, we propose that delirium is a potentially modifiable risk factor for dementi

On Star Formation and the Non-Existence of Dark Galaxies

We investigate whether a baryonic dark galaxy or `galaxy without stars' could persist indefinitely in the local universe, while remaining stable against star formation. To this end, a simple model has been constructed to determine the equilibrium distribution and composition of a gaseous protogalactic disk. Specifically, we determine the amount of gas that will transit to a Toomre unstable cold phase via the H2 cooling channel in the presence of a UV--X-ray cosmic background radiation field. All but one of the models are predicted to become unstable to star formation. Moreover, we find that all our model objects would be detectable via HI line emission, even in the case that star formation is potentially avoided. These results are consistent with the non-detection of isolated extragalactic HI clouds with no optical counterpart (galaxies without stars) by HIPASS. Additionally, where star formation is predicted to occur, we determine the minimum interstellar radiation field required to restore gravothermal stability, which we then relate to a minimum global star formation rate. This leads to the prediction of a previously undocumented relation between HI mass and star formation rate that is observed for a wide variety of dwarf galaxies in the HI mass range 10^8--10^10 M_sun. The existence of such a relation strongly supports the notion that the well observed population of dwarf galaxies represent the minimum rates of self-regulating star formation in the universe. (Barely abridged)Comment: 19 pages, 8 figures, TeX using emulateapj.cls, v2 accepted for publication in ApJ (16/8/5) with one figure deleted and a number of minor clarifying revision

ASAS-SSR Triennnial Reproduction Symposium: Looking Back and Moving Forward—How Reproductive Physiology has Evolved: Fetal origins of impaired muscle growth and metabolic dysfunction: Lessons from the heat-stressed pregnant ewe

Intrauterine growth restriction (IUGR) is the second leading cause of perinatal mortality and predisposes offspring to metabolic disorders at all stages of life. Muscle-centric fetal adaptations reduce growth and yield metabolic parsimony, beneficial for IUGR fetal survival but detrimental to metabolic health after birth. Epidemiological studies have reported that IUGRborn children experience greater prevalence of insulin resistance and obesity, which progresses to diabetes, hypertension, and other metabolic disorders in adulthood that reduce quality of life. Similar adaptive programming in livestock results in decreased birth weights, reduced and inefficient growth, decreased carcass merit, and substantially greater mortality rates prior to maturation. High rates of glucose consumption and metabolic plasticity make skeletal muscle a primary target for nutrient-sparing adaptations in the IUGR fetus, but at the cost of its contribution to proper glucose homeostasis after birth. Identifying the mechanisms underlying IUGR pathophysiology is a fundamental step in developing treatments and interventions to improve outcomes in IUGR-born humans and livestock. In this review, we outline the current knowledge regarding the adaptive restriction of muscle growth and alteration of glucose metabolism that develops in response to progressively exacerbating intrauterine conditions. In addition, we discuss the evidence implicating developmental changes in β adrenergic and inflammatory systems as key mechanisms for dysregulation of these processes. Lastly, we highlight the utility and importance of sheep models in developing this knowledge

Sustained maternal inflammation during the early third trimester yields fetal adaptations that impair subsequent skeletal muscle growth and glucose metabolism in sheep

Intrauterine growth restriction (IUGR) is linked to metabolic dysfunction in offspring, but the mediating mechanisms are still under investigation (Barker et al., 1993). IUGR fetuses adapt to their poor intrauterine environment by repartitioning nutrients to organs critical for survival (i.e., brain, heart) at the expense of tissues such as muscle (Yates et al., 2012c). These developmental adaptations help the fetus to survive in utero but have lifelong consequences in offspring; persistent reduction of highly metabolic muscle mass is detrimental to glucose homeostasis (DeFronzo et al., 1981). Glucose metabolism is regulated primarily by insulin, and nutrient depravation is associated with impaired β-cell mass, insulin secretion, and insulin action in the IUGR fetus (Limesand et al., 2006). Moreover, inflammation disrupts insulin action and aids in the development of insulin resistance (Bach et al., 2013). We recently showed that inflammatory cytokines acutely stimulate glucose metabolism despite their antagonistic effects on insulin signaling (Cadaret et al., 2017b). However, we hypothesize that chronic exposure alters responsiveness to cytokines and results in basal cytokine concentrations having a greater inhibitory tone. Furthermore, chronic maternal inflammation may induce fetal inflammatory adaptations that impair muscle growth and metabolism. Therefore, our objective was to determine the effects of sustained maternal inflammation on fetal growth, islet function, and muscle glucose metabolism

Sustained maternal inflammation during the early third trimester yields fetal adaptations that impair subsequent skeletal muscle growth and glucose metabolism in sheep

Intrauterine growth restriction (IUGR) is linked to metabolic dysfunction in offspring, but the mediating mechanisms are still under investigation (Barker et al., 1993). IUGR fetuses adapt to their poor intrauterine environment by repartitioning nutrients to organs critical for survival (i.e., brain, heart) at the expense of tissues such as muscle (Yates et al., 2012c). These developmental adaptations help the fetus to survive in utero but have lifelong consequences in offspring; persistent reduction of highly metabolic muscle mass is detrimental to glucose homeostasis (DeFronzo et al., 1981). Glucose metabolism is regulated primarily by insulin, and nutrient depravation is associated with impaired β-cell mass, insulin secretion, and insulin action in the IUGR fetus (Limesand et al., 2006). Moreover, inflammation disrupts insulin action and aids in the development of insulin resistance (Bach et al., 2013). We recently showed that inflammatory cytokines acutely stimulate glucose metabolism despite their antagonistic effects on insulin signaling (Cadaret et al., 2017b). However, we hypothesize that chronic exposure alters responsiveness to cytokines and results in basal cytokine concentrations having a greater inhibitory tone. Furthermore, chronic maternal inflammation may induce fetal inflammatory adaptations that impair muscle growth and metabolism. Therefore, our objective was to determine the effects of sustained maternal inflammation on fetal growth, islet function, and muscle glucose metabolism

Entropy Production in Collisionless Systems. I. Large Phase-Space Occupation Numbers

Certain thermal non-equilibrium situations, outside of the astrophysical realm, suggest that entropy production extrema, instead of entropy extrema, are related to stationary states. In an effort to better understand the evolution of collisionless self-gravitating systems, we investigate the role of entropy production and develop expressions for the entropy production rate in two particular statistical families that describe self-gravitating systems. From these entropy production descriptions, we derive the requirements for extremizing the entropy production rate in terms of specific forms for the relaxation function in the Boltzmann equation. We discuss some implications of these relaxation functions and point to future work that will further test this novel thermodynamic viewpoint of collisionless relaxation.Comment: accepted for publication in Ap