Physiology of L-asparaginase Synthesis by Escherichia Coli

A mating between Escherichia coli 4318 (thi-leu-Las-Hfr) and E. coli A-1 (Met-Las+ F-) resulted in the formation of phototrophic recombinantshaving L-asparaginase activities at three distinct levels. The physiology of L-asparaginase synthesis in these recombinants is described

Degradation of Homologous Polymerized Deoxyribonucleic Acid by Azotobacter Vinelandii ATCC 12837

The purpose of this study was twofold. The first was to isolate, purify, and characterize the deoxyribonucleic acid (DNA) of Azotobacter vinelandii ATCO 12837. The second was to determine if there was irreversible binding of homologous 32P labeled DNA to recipient A. vinelandii cells

Becoming a Highly Effective Elementary Teacher and How to Support Teachers\u27 Development

As teachers enter the field of teaching, teachers look to continue to develop in order to become highly effective teachers as they are often less effective than teachers with at least some experience (Boyd et al., 2008; Ladd & Sorenson, 2017). This development may occur through the support of mentors, administration, professional development (PD), as well as through trial and error, with the most development occurring during the first several years of teaching (Koedel & Betts, 2007; Rice, 2003). However, not all teachers develop into effective teachers. Some aspects that may affect a teacher’s effectiveness are the classroom environment, instruction, and pedagogy, which then may affect student engagement and achievement based on if they are effective or ineffective (Stronge, 2018). Though having a positive impact on student engagement in the classroom is essential, others have gone further to state effective teachers do much more (Roehrig et al., 2012). More recently, along with observations of teachers, the use of value-added scores linked student achievement to teacher effectiveness (Goe, 2007). However, this approach to evaluating teachers is controversial, especially among teachers (Papay, 2011; Pressley et al., 2018; Rothstein, 2009). Because of the difference between highly effective teachers and others, it is important to understand how highly effective teachers have had success within the classroom. The purpose of this study was to provide insight into the development of a highly effective teacher. The researchers looked to answer the following research questions: 1) What behaviors are new and developing teachers doing that make them less effective teachers? 2) How can schools support new and developing teachers to develop into highly effective teachers? and 3) How do new, developing, and highly effective teachers approach professional development to improve teaching

Piano Night at the Movies: A Recital

This is the poster and program from the recital, Piano Night at the Movies, held on October 30, 2020, at the OBU Amphitheater

Regional policy spillovers : the national impact of demand-side policy in an interregional model of the UK economy

UK regional policy has been advocated as a means of reducing regional disparities and stimulating national growth. However, there is limited understanding of the interregional and national effects of such a policy. This paper uses an interregional computable general equilibrium model to identify the national impact of a policy-induced regional demand shock under alternative labour market closures. Our simulation results suggest that regional policy operating solely on the demand side has significant national impacts. Furthermore, the effects on the nontarget region are particularly sensitive to the treatment of the regional labour market

Designing a Locally Manufacturable Wheelchair for Nepal

Persons with disabilities in developing countries often lack the basic equipment needed to assist them in their daily lives. International Nepal Fellowship (INF) is a Christian medical organization located in Nepal that provides medical care and assistance to people with disabilities and other conditions. Currently, INF imports expensive wheelchairs that undergo a prolonged border process before being received. INF has reached out to the Collaboratory to design a wheelchair that can withstand the challenges of Nepal’s terrain and can be manufactured from local materials. The Nepal Wheelchair team accepted this challenge and set out to design a wheelchair that can fulfill their needs. The team began by researching wheelchair models for inspiration and eventually settled on two preliminary designs. In January 2020, the team traveled to Pokhara, Nepal to gain feedback from the staff at INF and select a final design based on their comments. During this trip, the team acquired and brought back locally available materials and parts available in Nepal in order to construct a prototype. This year, prototyping has been completed for the following elements: the central frame, wheel lock, footrest, wheel mounting assemblies and armrest fixtures. As a result of knowledge gained during prototyping, some design changes to the wheelchair have been made. Moving forward, the team will test the overall prototype for durability and prepare a manufacturing manual for INF.https://mosaic.messiah.edu/engr2021/1010/thumbnail.jp

Parasite-Derived Plasma Microparticles Contribute Significantly to Malaria Infection-Induced Inflammation through Potent Macrophage Stimulation

There is considerable debate as to the nature of the primary parasite-derived moieties that activate innate pro-inflammatory responses during malaria infection. Microparticles (MPs), which are produced by numerous cell types following vesiculation of the cellular membrane as a consequence of cell death or immune-activation, exert strong pro-inflammatory activity in other disease states. Here we demonstrate that MPs, derived from the plasma of malaria infected mice, but not naive mice, induce potent activation of macrophages in vitro as measured by CD40 up-regulation and TNF production. In vitro, these MPs induced significantly higher levels of macrophage activation than intact infected red blood cells. Immunofluorescence staining revealed that MPs contained significant amounts of parasite material indicating that they are derived primarily from infected red blood cells rather than platelets or endothelial cells. MP driven macrophage activation was completely abolished in the absence of MyD88 and TLR-4 signalling. Similar levels of immunogenic MPs were produced in WT and in TNF−/−, IFN-γ−/−, IL-12−/− and RAG-1−/− malaria-infected mice, but were not produced in mice injected with LPS, showing that inflammation is not required for the production of MPs during malaria infection. This study therefore establishes parasitized red blood cell-derived MPs as a major inducer of systemic inflammation during malaria infection, raising important questions about their role in severe disease and in the generation of adaptive immune responses

Isolation of Anti-Inflammatory and Epithelium Reinforcing Bacteroides and Parabacteroides Spp. from A Healthy Fecal Donor

Altered intestinal microbiota is associated with systemic and intestinal diseases, such as inflammatory bowel disease (IBD). Dysbiotic microbiota with enhanced proinflammatory capacity is characterized by depletion of anaerobic commensals, increased proportion of facultatively anaerobic bacteria, as well as reduced diversity and stability. In this study, we developed a high-throughput in vitro screening assay to isolate intestinal commensal bacteria with anti-inflammatory capacity from a healthy fecal microbiota transplantation donor. Freshly isolated gut bacteria were screened for their capacity to attenuate Escherichia coli lipopolysaccharide (LPS)-induced interleukin 8 (IL-8) release from HT-29 cells. The screen yielded a number of Bacteroides and Parabacteroides isolates, which were identified as P. distasonis, B. caccae, B. intestinalis, B. uniformis, B. fragilis, B. vulgatus and B. ovatus using whole genome sequencing. We observed that a cell-cell contact with the epithelium was not necessary to alleviate in vitro inflammation as spent culture media from the isolates were also effective and the anti-inflammatory action did not correlate with the enterocyte adherence capacity of the isolates. The anti-inflammatory isolates also exerted enterocyte monolayer reinforcing action and lacked essential genes to synthetize hexa-acylated, proinflammatory lipid A, part of LPS. Yet, the anti-inflammatory effector molecules remain to be identified. The Bacteroides strains isolated and characterized in this study have potential to be used as so-called next-generation probiotics

Isolation of Anti-Inflammatory and Epithelium Reinforcing Bacteroides and Parabacteroides Spp. from A Healthy Fecal Donor

Altered intestinal microbiota is associated with systemic and intestinal diseases, such as inflammatory bowel disease (IBD). Dysbiotic microbiota with enhanced proinflammatory capacity is characterized by depletion of anaerobic commensals, increased proportion of facultatively anaerobic bacteria, as well as reduced diversity and stability. In this study, we developed a high-throughput in vitro screening assay to isolate intestinal commensal bacteria with anti-inflammatory capacity from a healthy fecal microbiota transplantation donor. Freshly isolated gut bacteria were screened for their capacity to attenuate Escherichia coli lipopolysaccharide (LPS)-induced interleukin 8 (IL-8) release from HT-29 cells. The screen yielded a number of Bacteroides and Parabacteroides isolates, which were identified as P. distasonis, B. caccae, B. intestinalis, B. uniformis, B. fragilis, B. vulgatus and B. ovatus using whole genome sequencing. We observed that a cell-cell contact with the epithelium was not necessary to alleviate in vitro inflammation as spent culture media from the isolates were also effective and the anti-inflammatory action did not correlate with the enterocyte adherence capacity of the isolates. The anti-inflammatory isolates also exerted enterocyte monolayer reinforcing action and lacked essential genes to synthetize hexa-acylated, proinflammatory lipid A, part of LPS. Yet, the anti-inflammatory effector molecules remain to be identified. The Bacteroides strains isolated and characterized in this study have potential to be used as so-called next-generation probiotics