Late-acting self-incompatible system, preferential allogamy and delayed selfing in the heteromorphic invasive populations of <i>Ludwigia grandiflora subsp. hexapetala</i>

International audienc

Etude de facteurs environnementaux et genetiques de l'androgenese in vitro chez le maies (Zea mays L.). Caracterisation des lignees haploiedes doublees

SIGLEINIST T 72771 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Challenges of genomic data generation for non-model complex species

International audienc

Challenges of genomic data generation for non-model complex species

International audienc

Réponses des plantes invasives [i]Ludwigia grandiflora[/i] et [i]Ludwigia peploides[/i] sous conditions de stress hydrique et salin

absen

GenAPoPop1.0: Computing population genetic indices and inferring reproductive modes from genotype diversity in polyploid populations.

International audienceUnderstanding the ecological and evolutionary impacts of polyploidy, especially on reproductive modes, implies to be able to easily compute population genetic indices and to quantitatively infer the respective rates of clonality, selfing and outcrossing producing current descendants

GenAPoPop1.0: Computing population genetic indices and inferring reproductive modes from genotype diversity in polyploid populations.

International audienceUnderstanding the ecological and evolutionary impacts of polyploidy, especially on reproductive modes, implies to be able to easily compute population genetic indices and to quantitatively infer the respective rates of clonality, selfing and outcrossing producing current descendants

Réponses des plantes invasives [i]Ludwigia grandiflora[/i] et [i]Ludwigia peploides[/i] sous conditions de stress hydrique et salin

absen

Etude de la plasticité de la plante aquatique invasive [i]Ludwigia grandiflora[/i] en milieu terrestre

Session 1 : Scénarios et Potentiel InvasifNational audienc

Sequencing, de novo assembly of Ludwigia plastomes, and comparative analysis within the Onagraceae family

Background: The Onagraceae family, which belongs to the order Myrtales, consists of approximately 657 species and 17 genera, including the genus Ludwigia L., which is comprised of 82 species. There are few genomic resources for Onagraceae, which limits phylogenetic and population genetics, as well as genomic studies. In this study, new complete plastid genomes of Ludwigia grandiflora subps. hexapetala (Lgh) and Ludwigia peploides subsp montevidensis (Lpm) were generated using a combination of different sequencing technologies. These plastomes were then compared to the published Ludwigia octovalvis (Lo) plastid genome, which was re-annotated by the authors. Results: We initially sequenced and assembled the chloroplast (cp) genomes of Lpm and Lgh using a hybrid strategy. We observed the existence of two Lgh haplotypes and two potential Lpm haplotypes. Lgh, Lpm, and Lo plastomes were similar in terms of genome size, gene number, structure, and inverted repeat (IR) boundaries, comparable to other species in the Myrtales order. A total of 45 to 65 SSRs (simple sequence repeats), were detected, depending on the species, with the majority consisting solely of A and T, which is common among angiosperms. Four chloroplast genes (matK, accD, ycf2 and ccsA) were found under positive selection pressure, which is commonly associated with plant development, and especially in aquatic plants such as Lgh, and Lpm. Conclusion: Our hybrid sequencing approach revealed the presence of two Lgh plastome haplotypes which will help to advance phylogenetic and evolutionary studies, not only specifically for Ludwigia, but also the Onagraceae family and Myrtales order. To enhance the robustness of our findings, a larger dataset of chloroplast genomes would be beneficial