Electrophoretic separation of human kidney cells at zero gravity

Electrophoretic isolation of cells results in a loss of resolution power caused by the sedimentation of the cells in the media. The results of an experiment to extract urokinase from human embryos during the Apollo Soyuz mission are presented and discussed

Evidence for Rotation in the Galaxy at z=3.15 Responsible for a Damped Lyman-alpha Absorption System in the Spectrum of Q2233+1310

Proof of the existence of a significant population of normal disk galaxies at redshift z>2 would have profound implications for theories of structure formation and evolution. We present evidence based on Keck HIRES observations that the damped Lyman-alpha absorber at z=3.15 toward the quasar Q2233+1310 may well be such an example. Djorgovski et al have recently detected the Lyman-alpha emission from the absorber, which we assume is at the systemic redshift of the absorbing galaxy. By examining the profiles of the metal absorption lines arising from the absorbing galaxy in relation to its systemic redshift, we find strong kinematical evidence for rotation. Therefore the absorber is likely to be a disk galaxy. The inferred circular velocity for the galaxy is >200 km/s. With a separation of ~17 kpc between the galaxy and the quasar sightline, the implied dynamic mass for the galaxy is >1.6x10(11) solar mass. The metallicity of the galaxy is found to be [Fe/H]=-1.4, typical of damped Lyman-alpha galaxies at such redshifts. However, in another damped galactic rotation is evident. In the latter case, the damped Lyman-alpha absorber occurs near the background quasar in redshift so its properties may be influenced by the background quasar. These represent the only two cases at present for which the technique used here may be applied. Future applications of the same technique to a large sample of damped Lyman-alpha galaxies may allow us to determine if a significant population of disk galaxies already existed only a few billion years after the Big Bang.Comment: AASTEX, 2 PS figures, accepted by ApJ, 6 pages total, replaced on 1-22-97, the only change is the enlarged figure

Atomic and molecular interstellar absorption lines toward the high galactic latitude stars HD~141569 and HD~157841 at ultra-high resolution

We present ultra-high resolution (0.32 km/s) spectra obtained with the 3.9m Anglo-Australian Telescope (AAT) and Ultra-High-Resolution Facility (UHRF), of interstellar NaI D1, D2, Ca II K, K I and CH absorption toward two high galactic latitude stars HD141569 and HD157841. We have compared our data with 21-cm observations obtained from the Leiden/Dwingeloo HI survey. We derive the velocity structure, column densities of the clouds represented by the various components and identify the clouds with ISM structures seen in the region at other wavelengths. We further derive abundances, linear depletions and H2 fractional abundances for these clouds, wherever possible. Toward HD141569, we detect two components in our UHRF spectra : a weak, broad component at - 15 km/s, seen only in CaII K absorption and another component at 0 km/s, seen in NaI D1, D2, Ca II K, KI and CH absorption. In the case of the HD157841 sightline, a total of 6 components are seen on our UHRF spectra in NaI D1, D2 Ca II K, K I and CH absorption. 2 of these 6 components are seen only in a single species.Comment: 16 pages, Latex, 4 figures, ps files Astrophysical Journal (in press

Persistent barriers and facilitators to seeking help for a dementia diagnosis: a systematic review of 30 years of the perspectives of carers and people with dementia

Objective: To identify barriers and facilitators to help seeking for a dementia diagnosis from the perspective of carers and people with dementia. Design: A systematic review of the literature was conducted according to the PRISMA guidelines (PROSPERO protocol registration CRD42018092524). Nine electronic databases were searched for qualitative, quantitative, and mixed methods primary research studies. Two independent reviewers screened titles and abstracts, full texts of eligible studies, and conducted quality appraisal of included articles. A convergent qualitative synthesis approach was used. Results: From 7496 articles, 35 papers representing 32 studies from 1986 to 2017 were included. Studies originated from 13 countries across 4 continents. Barriers and facilitators were reported predominantly by carers. A small number of studies included people with dementia. Barriers included denial, stigma and fear, lack of knowledge, normalization of symptoms, preserving autonomy, lack of perceived need, unaware of changes, lack of informal network support, carer difficulties, and problems accessing help. Facilitators included recognition of symptoms as a problem, prior knowledge and contacts, and support from informal network. Conclusions: Studies from a 30-year period demonstrated that barriers to help seeking persist globally, despite increasing numbers of national dementia policies. Barriers and facilitators rarely existed independently demonstrating the complexity of help seeking for a diagnosis of dementia. Multiple barriers compounded the decision-making process and more than one facilitator was often required to overcome them. Multi-faceted interventions to reduce barriers are needed, one approach would be a focus on the development of dementia friendly communities to reduce stigma and empower people with dementia and carers

Struggling and juggling: a comparison of assessment loads in research and teaching-intensive universities

In spite of the rising tide of metrics in UK higher education, there has been scant attention paid to assessment loads, when evidence demonstrates that heavy demands lead to surface learning. Our study seeks to redress the situation by defining assessment loads and comparing them across research-and teaching intensive universities. We clarify the concept of ‘assessment load’ in response to findings about high volumes of summative assessment on modular degrees. We define assessment load across whole undergraduate degrees, according to four measures: the volume of summative assessment; volume of formative assessment; proportion of examinations to coursework; number of different varieties of assessment. All four factors contribute to the weight of an assessment load, and influence students’ approaches to learning. Our research compares programme assessment data from 73 programmes in 14 UK universities, across two institutional categories. Research-intensives have higher summative assessment loads and a greater proportion of examinations; teaching-intensives have higher varieties of assessment. Formative assessment does not differ significantly across both university groups. These findings pose particular challenges for students in different parts of the sector. Our study questions the wisdom that ‘more’ is always better, proposing that lighter assessment loads may make room for ‘slow’ and deep learning

Communications Biophysics

Contains reports on five research projects.United States Air Force (Contract AF19(604)-4112)National Institute of Neurological Diseases and Blindness (B369 Physiology

Hubble Space Telescope Images of Magellanic Cloud Planetary Nebulae: Data and Correlations across Morphological Classes

The morphology of planetary nebulae (PNe) provides an essential tool for understanding their origin and evolution, as it reflects both the dynamics of the gas ejected during the TP-AGB phase, and the central star energetics. Here we study the morphology of 27 Magellanic Cloud planetary nebulae (MCPNe) and present an analysis of their physical characteristics across morphological classes. Similar studies have been successfully carried out for galactic PNe, but were compromised by the uncertainty of individual PN distances. We present our own HST/FOC images of 15 Magellanic Cloud PNe (MCPNe) acquired through a narrow-band lambda 5007 [O III] filter. We use the Richardson-Lucy deconvolution technique on these pre-COSTAR images to achieve post-COSTAR quality. Three PNe imaged before and after COSTAR confirm the high reliability of our deconvolution procedure. We derive morphological classes, dimensions, and surface photometry for all these PNe. We have combined this sample with HST/PC1 images of 15 MCPNe, three of which are in common with the FOC set, acquired by Dopita et al. (1996), to obtain the largest MCPN sample ever examined from the morphological viewpoint. By using the whole database, supplemented with published data from the literature, we have analyzed the properties of the MCPNe and compared them to a typical, complete galactic sample. Morphology of the MCPNe is then correlated with PN density, chemistry, and evolution.Comment: text file lstanghe_mcpn.tex (LaTex); Figures 2 through 10, Figure 5 is in 3 parts (a,b,c); Figure 1 available by regular mail only; ApJ, in press, November 10, 199

Temperature and Kinematics of CIV Absorption Systems

We use Keck HIRES spectra of three intermediate redshift QSOs to study the physical state and kinematics of the individual components of CIV selected heavy element absorption systems. Fewer than 8 % of all CIV lines with column densities greater than 10^{12.5} cm^{-2} have Doppler parameters b < 6 km/s. A formal decomposition into thermal and non-thermal motion using the simultaneous presence of SiIV gives a mean thermal Doppler parameter b_{therm}(CIV) = 7.2 km/s, corresponding to a temperature of 38,000 K although temperatures possibly in excess of 300,000 K occur occasionally. We also find tentative evidence for a mild increase of temperature with HI column density. Non-thermal motions within components are typically small (< 10 km/s) for most systems, indicative of a quiescent environment. The two-point correlation function (TPCF) of CIV systems on scales up to 500 km/s suggests that there is more than one source of velocity dispersion. The shape of the TPCF can be understood if the CIV systems are caused by ensembles of objects with the kinematics of dwarf galaxies on a small scale, while following the Hubble flow on a larger scale. Individual high redshift CIV components may be the building blocks of future normal galaxies in a hierarchical structure formation scenario.Comment: submitted to the ApJ Letters, March 16, 1996 (in press); (13 Latex pages, 4 Postscript figures, and psfig.sty included