PENINGKATAN PERILAKU SADAR PERIODONTAL SEHAT BAGI IBU HAMIL DI MASA PANDEMI COVID-19

Salah satu dampak nyata dari adanya pandemi Covid-19 dan kebijakan bekerja dari rumah adalah peningkatan angka kehamilan di masyarakat. Jawa Timur sebagai salah satu provinsi dengan jumlah penduduk terbanyak di Indonesia menunjukkan peningkatan angka kehamilan selama masa pandemi. Tingkat kelahiran prematur di Jawa Timur sebesar 23.3%, sementara pada tahun 2018, 885 kelahiran bayi di Surabaya memiliki berat badan lahir rendah (BBLR). Salah satu faktor resiko dari kelahiran bayi prematur dan BBLR (berat badan lahir rendah) adalah adanya kelainan jaringan rongga mulut yaitu kelainan periodontal. Kelainan periodontal yang umum ditemukan pada ibu hamil diantaranya adalah gingivitis dan periodontitis. Di Kota Surabaya, studi yang dilakukan pada delapan puskesmas menunjukkan 73% ibu hamil mengalami gingivitis dan 36% ibu hamil mengalami periodontitis. Mengingat pentingnya kesehatan periodontal, gigi dan mulut selama masa kehamilan, pada pengabdian masyarakat kali ini dilakukan sosialisasi kesehatan jaringan periodontal, gigi dan mulut pada ibu hamil. Target sasaran dari pengabdian masyarakat ini adalah ibu hamil yang melakukan kontrol kehamilan pada klinik utamaarea kecamatan Tambak Sari. Pengabdian masyarakat dilakukan melalui metode pemutaran video, ceramah, diskusi dan peragaan prosedur kesehatan periodontal dan rongga mulut. Keberhasilan dari program pengabdian masyarakat ini dinilai melalui indikator peningkatan nilai pada tes tertulis yang diberikan sebelum dan sesudah pelaksanaan sosialisasi. Melalui pengabdian masyarakat ini, pengetahuan dan prilaku sadar periodontal sehat bagi ibu hamil dapat ditingkatkan, sehingga diharapkan dapat mengurangi resiko penyakit periodontal bagi kehamilan

Daya hambat ekstrak biji kopi robusta (Coffea Canephora) terhadap pertumbuhan bakteri plak

Background. Plaque is the major causative factor for periodontal disease, such as gingivitis and eriodontitis. Local antibacterial substancescan reduce plaque accumulation. Many herbs has antibacterial properties. One of them is Robusta coffee. Robusta coffee contains caffeine, polyphenols, and trigonelline, which has ability to lead to bacteria cell death. Purpose. The aim of this study was to determine the inhibition of Robusta coffee bean extracton bacterial plaque growth. Method. This study was using Soxhlet technique to prepared Robusta coffee extract. Dilution techniques was used to form 8 concentration, 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, and 0,78%. Antibacterial effect of extract was studied by counting the number of growth colonies which growth on Mueller-Hinton Agar(MHA) medium.Results. Inhibition activity of Robusta coffee bean extract start from concentration 1,56%. Conclusion.Robusta coffee bean extract was effective to inhibit the growth of plaque bacteria colony from concentration 1,56%

Inhibitory activity of jackfruit (Atrocarpus heterophyllus Lam.) leaf extract on the growth of Porphyromonas gingivalis bacteria

Background: Periodontitis is an inflammation that affects the periodontal tissue, causing pocket formation, loss of attachment, and tooth mobility. Periodontitis caused by a complex interaction between the body's immune response and microorganisms in dental plaque. One of the main agents in the etiology of chronic periodontitis is Porphyromonas gingivalis. Jackfruit (Atrocarpus heterophyllus Lam.) leaf contains active compounds such as saponin, flavonoid, and tannin. Objective: This study is aimed to determine the inhibition of jackfruit leaf extract (Atrocarpus heterophyllus Lam.) on the growth of Porphyromonas gingivalis bacteria. Method: This research is an in vitro laboratory experiment. Jackfruit leaf extraction was carried out by maceration method with 70% ethanol solvent, then diluted serially by dilution to obtain various concentrations. Concentrations used include 100%; 50%; 25%; 12.5%; 6.25%; 3.125%; 1.56%; and 0.78%. Inhibition test against Porphyromonas gingivalis bacteria was carried out by spectrophotometric method and colony count. Result: Jackfruit leaf extract with a concentration of 12.5%; 25%; 50%; and 100% can kill Porphyromonas gingivalis bacterial colonies resulting in 0 colonies. A concentration of 6.25% inhibited the growth of bacteria by 91.77%. The results of statistical tests obtained a significance value of 0.001 (P <0.05). Conclusion: Jackfruit leaf extract (Atrocarpus heterophyllus Lam.) has an inhibitory effect on the growth of Porphyromonas gingivalis bacteria with MIC values at a concentration of 6.25% and MBC at a concentration of 12.5%

Viability Testof Lemongrass Extract (Cymbopogon Citratus) on BHK-21 Fibroblast Cell Culture

Background: Lemongrass are found in Indonesia thus it lemongrass can be used as an alternative in healing inflammation by conducting a preliminary study in the form of BHK-21 fibroblast cell viability test after 24 hours of extract lemongrass. Objective: To determine the viability of extract lemongrass against BHK-21 fibroblast cell cultures. Method: BHK-21 fibroblast cells were taken from neonatal hamsters’ kidneys and planted in 96 well plates. Extracts lemongrass with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.13% and 1.56% were included in each well prepared and incubated for 24 hours. MTT Assay was performed to see the viability of extract lemongrass on fibroblast cells. Results: Extract lemongrass viable against BHK-21 fibroblast cells at a concentration of 100%. There is a decrease in viability at concentrations of 50% to 1.56%. Conclusion: The 100% concentration showed the highest viability of BHK-21 fibroblast cells and extract lemongrass had potential in wound healing

Peningkatan Kesehatan Periodontal Ibu Hamil Dalam Upaya Mengurangi Resiko Bayi Dengan Berat Badan Lahir Rendah

Selama masa pandemi Covid-19, terjadi peningkatan tingkat kehamilan di Jawa Timur. Selain usaha menekan tingkat kehamilan, perlu juga dipastikan kesehatan ibu dan janin agar lahir dengan sehat. Prevalensi kelainan periodontal pada ibu hamil tinggi sementara kunjungan ibu hamil ke dokter gigi rendah. Penyakit periodontal merupakan salah satu faktor resiko bayi lahir prematur dan juga berat badan lahir rendah (BBLR). Dalam pengabdian masyarakat ini dilakukan sosialisasi melalui metode ceramah, tanya jawab, diskusi dan praktek mandiri prosedur menjaga kesehatan rongga mulut. Tujuannya adalah meningkatkan pengetahuan ibu hamil mengenai pentingnya perawatan gigi dan mulut khususnya jaringan periodontal selama kehamilan dan meningkatnya ketrampilan ibu hamil dalam mendeteksi gejala awal kelainan periodontal dan cara melakukan perawatan gigi mulut mandiri di rumah. Pada pengabdian masyarakat ini pengetahuan dan ketrampilan ibu hamil dapat ditingkatkan. dengan jaringan periodontal dan gigi serta rongga mulut yang sehat, diharapkan resiko BBLR maupun kelahiran prematur dapat diturunkan

OPG and RANKL Signal Transduction in Osteoblast Regulation Post Application Extract Collagen in Osteogenesis

Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneration. Important factor for periodontal regeneration is to promote bone formation, nowadays used bone replacement. The used of xenograft materials as gold standard for periodontal tissue regeneration using type I collagen bone graft has been widely developed. The main organic component in fish scales is type I fibril collagen, which are same as with component in bones. Specific markers of bone formation are the expression of osteoblast, osteoclast, osteoprotegerin (OPG), and receptor activator of nuclear factor κB ligands (RANKL). The purpose of this study is to assess the expression of OPG and RANKL after application of extract collagen from gourami (Osphronemus gouramy) scales. Materials and Methods: Thirty-two experimental 3-month-old male Wistar albino rats (150g and 200g) were randomly divided into four groups: 7-day control group, 7-day fish collagen group, 14-day control group and 14-day fish collagen group. The sockets were filled with blood (control group), whereas 10 mg to 30 mg extract collagen was added until the sockets were fully occupied (treatment group). Results: The ANOVA test showed a significance level of 0.000 (p <0.05). Conclusion: Expression of OPG enhanced and expression of RANKL lowered after application of type I collagen from gourami scales, accelerating osteogenesis

Viability of Nigella sativa Toothpaste with SLS Compared Non-SLS on Fibroblast Cell Culture

beneficial effects in infectious disease, such as gingival inflammation. One of the most widely used synthetic detergents in toothpaste is Sodium lauryl sulphate (SLS). SLS is used to decrease water's surface tension. The side effects of SLS include oral epithelial sloughing, ulcerations, inflammation, protein denaturation, and membrane expansion. This study aimed to determine the viability of human gingival fibroblast (HGFs) cultured with Nigella sativa toothpaste extract containing 2% SLS and Nigella sativa toothpaste without SLS. HGFs were grown in DMEM medium then challenged with Nigella sativa toothpaste with 2% SLS (Nigella sativa-SLS group), Nigella sativa toothpaste without SLS (non-SLS group), and control group. Based on the present study, the cell viability of Nigella sativa-2% SLS, non-SLS, and control group were 92.33%, 96.30%, and 98.99%, respectively. In conclusion, both Nigella sativa with 2% SLS and non-SLS were non-toxic to HGFs. Thus, Nigella sativa toothpaste is potential to treat gingivitis and oral diseases. However, future in vivo study for this is needed