Investigating the Effects of Several Parameters on the Growth of Chlorella vulgaris Using Taguchi's Experimental Approach

Algae are part of photosynthetic organisms that play an important role in the aquatics nutrition. Like plants, algae need water, light and CO2 to grow. Using Taguchi's experimental approach (5 factors in four levels with 16 runs), effects of several parameters (NaCl, sodium bicarbonate and iron concentrations as well as light and temperature) on the growth of Chlorella vulgaris was studied. Increasing the concentrations of NaCl and sodium bicarbonate resulted in corresponding decreases in the growth of C. vulgaris. Media with 30.0g l-1NaCl did not indicate any algal growth. Also, with 9.0 g l-1sodium bicarbonate, biomass production was decreased. Chlorella vulgaris showed different growing behaviors at the various concentrations of iron (Fe+2) and at the different temperatures of this study. Maximum biomass production (approximately 3.56 g dry matter) was obtained at the 0.0 g l-1 sodium bicarbonate, 10.0g l-1NaCl, 18.0 µmol l-1 iron and at 30±2 °C. Yellow and blue lights increased the algal growth. Analysis of variance showed that salinity (i.e., the NaCl concentration) had the highest impact on the biomass production.

New foci of zoonotic cutaneous leishmaniosis due to Leishmania major in the northeastern Iran cities of Sabzevar and Neghaab

Cutaneous leishmaniosis (CL) is a major public health challenge in Iran. Overall, 18 provinces out of 31 of the country's provinces are in the endemic areas. The objective of the present study was to determine the presence of CL species in Sabzevar and Neghaab cities, Khorasan Razavi province, northeastern Iran. Overall, 280 samples were taken from suspected individuals referred to Sabzevar's and Neghaab's health centers during 2014-2016. Smear preparation, case detection and further identification by partial amplification of 7SL RNA and high-resolution melting curve analysis were done for all samples, followed by randomly polymerase chain reaction (PCR)-sequencing confirmation. Based on findings, the most infection rate was found in males with the age groups of 20-30 in both districts. All samples were characterized as Leishmania major, except one isolate in Neghaab closely related to Leishmania tropica. Various risk factors play roles in creating new foci of zoonotic CL (ZCL) caused by L. major in Sabzevar and Neghaab in northeastern Iran. Reports of the prevalence of CL in new foci make serious concerns about the incidence of cases and expansion of disease to the neighboring areas. Further measures are essential to control the disease in the regions