36 research outputs found
Neuroprotective strategy for Alzheimer disease: intranasal administration of a fatty neuropeptide.
No full textPatterning Gradient Properties from Sub-Micrometers to Millimeters by Magnetolithography
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Aptamer-antibody sandwich assay for cytochrome c employing an MWCNT platform and electrochemical impedance
No full textWe report on a sensitive aptamer-antibody interaction-based assay for cytochrome c (Cytc) using electrochemical impedance. 4-Amino benzoic acid is used for the oriented immobilization of aminated aptamers onto multi-walled carbon nanotubes on the surface of a screen-printed electrode via electrochemical grafting. Impedance was measured in a solution containing the redox system ferro/ferricyanide. The change in interfacial charge transfer resistance (Rct) experienced by the redox marker was recorded to confirm the formation of a complex between aptamer and the target (Cytc). A biotinylated antibody against cytochrome c was then used in a sandwich type of assay. The addition of streptavidin conjugated to gold nanoparticles and signal enhancement by treatment with silver led to a further increase in Rct. Under optimized conditions, a detection limit as low as 12 pM was obtained. Cross-reactivity against other serum proteins including fibrinogen, BSA and immunoglobulin G demonstrated improved selectivity
Reconstitution of Apo-Glucose Oxidase on Nitrospiropyran and FAD Mixed Monolayers on Gold Electrodes: Photostimulation of Bioelectrocatalytic Features of the Biocatalyst
No full textInterdigitated Array Microelectrode-Based Electrochemical Impedance Immunosensor for Detection of Escherichia
No full textAssociation of Anti-Dinitrophenyl Antibody onto a Patterned Organosiloxane Antigen Monolayer Prepared by Microcontact Printing: An AFM Characterization
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