Variaciones temporales del contenido en ácidos grasos de leche de oveja

El presente trabajo ha consistido en la estimación de la repetibilidad tem- poral diaria, semanal y mensual, del contenido de los ácidos grasos de la leche de oveja en el curso de una lactación. Se ha realizado la cuantificación para 36 ácidos grasos y 6 agrupaciones en 596 muestras de leche procedente de 32 ovejas de las razas Churra y Assaf. Las bajas repetibilidades, en aquellos ácidos con muy baja concentración, son debido a un efecto de falta de precisión en su cuantificación, originado por el bajo contenido de dicho ácido graso. La concentración de ácidos grasos en la leche ovina, presenta una variación a lo largo de la lactación que es proporcional a las distancia entre controles. Para las variables cuantitativamente importantes, las repetibilidades medias diarias, semanales y mensuales fueron 0,55, 0,46 y 0,32 respectivamente

Induction of a gloverin-like antimicrobial polypeptide in the sugarcane borer Diatraea saccharalis challenged by septic injury

Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) is an important pest for Brazilian sugarcane. In the present study, we detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both spots were subjected to in-gel tryptic digestion and MALDI-TOF/TOF analysis, which revealed the sequence VFGTLGSDDSGLFGK present in both HDs1 and HDs2. This sequence had homology and 80% identity with specific Lepidoptera antimicrobial peptides called gloverins. Analyses using the ImageMaster 2D software showed pI 8.94 of the HDs1 spot, which is similar to that described to Hyalophora gloveri gloverin (pI 8.5). Moreover, the 14-kDa molecular mass of the spot HDs1 is compatible to that of gloverins isolated from the hemolymph of Trichoplusia ni, Helicoverpa armigera and H. gloveri. Antimicrobial assays with partially purified fractions containing the HDs1 and HDs2 polypeptides demonstrated activity against Escherichia coli. This is the first report of antimicrobial polypeptides in D. saccharalis, and the identification of these peptides may help in the generation of new strategies to control this pest

Economic and climatic models for estimating coffee supply

O objetivo deste trabalho foi estimar a oferta cafeeira por meio da calibração de modelos estatísticos, com variáveis econômicas e climáticas, das principais regiões produtoras do Estado de São Paulo. As regiões estudadas foram Batatais, Caconde, Cássia dos Coqueiros, Cristais Paulista, Espírito Santo do Pinhal, Marília, Mococa e Osvaldo Cruz. Foram utilizados dados de oferta cafeeira, variáveis econômicas (crédito rural, crédito rural na agricultura e valor da produção) e variáveis climáticas (temperatura do ar, precipitação pluvial, evapotranspiração potencial, deficiência e excedente hídrico) de cada região, para o período de 2000–2014. Os modelos foram calibrados com uso de técnicas de regressão linear múltipla, e todas as combinações possíveis foram testadas para a seleção das variáveis. A oferta cafeeira foi a variável dependente, e as demais, as independentes. A acurácia e a precisão dos modelos foram analisadas pelo erro percentual médio e pelo coeficiente de determinação ajustado, respectivamente. As variáveis que mais influenciam a oferta cafeeira são o valor de produção e a temperatura do ar. É possível estimar a oferta cafeeira com regressões lineares múltiplas por meio de variáveis econômicas e elementos climáticos. Os modelos mais acurados são os calibrados para estimar a oferta cafeeira das regiões de Cássia dos Coqueiros e Osvaldo Cruz.The objective of this work was to estimate the coffee supply by calibrating statistical models with economic and climatic variables for the main producing regions of the state of São Paulo, Brazil. The regions were Batatais, Caconde, Cássia dos Coqueiros, Cristais Paulista, Espírito Santo do Pinhal, Marília, Mococa, and Osvaldo Cruz. Data on coffee supply, economic variables (rural credit, rural agricultural credit, and production value), and climatic variables (air temperature, rainfall, potential evapotranspiration, water deficit, and water surplus) for each region, during the period from 2000–2014, were used. The models were calibrated using multiple linear regression, and all possible combinations were tested for selecting the variables. Coffee supply was the dependent variable, and the other ones were considered independent. The accuracy and precision of the models were assessed by the mean absolute percentage error and the adjusted coefficient of determination, respectively. The variables that most affect coffee supply are production value and air temperature. Coffee supply can be estimated with multiple linear regressions using economic and climatic variables. The most accurate models are those calibrated to estimate coffee supply for the regions of Cássia dos Coqueiros and Osvaldo Cruz

Pharmacological activity of the hydroalcoholic extract from Hovenia dulcis thunberg fruit and the flavonoid dihydromyricetin during hypercholesterolemia induced in rats

Cerebrovascular accidents and coronary artery disease are the leading causes of cardiovascular mortalities in Brazil and high levels of LDL cholesterol are one of the main risk factors. In this context, several plant extracts and natural substances have shown promise as cholesterol-lowering. The objective of this study was to evaluate the potential of the hydroalcoholic extract of the fruit of H. dulcisand of dihydromyricetin in cholesterol reduction in hypercholesterolemic rats. Forty-two Wistar male rats were distributed into seven groups of six animals that received diets supplemented with 1% cholesterol and 0.3% cholic acid, with the exception of the control group, which received conventional diets. Animals were treated with oral suspensions containing: atorvastatin 1.0 mg/kg; H. dulcisextract at 50.0 and 100.0 mg/kg and dihydromyricetin at 25.0 and 50.0 mg/kg vehicle (control group). The following biochemical markers were evaluated; total cholesterol, HDL-C, LDL-C, triglycerides, AST, ALT, and alkaline phosphatase. The hypercholesterolemic diet was effective in inducing hypercholesterolemia, increasing total cholesterol by 112.7% relative to the control group. The treatments with two doses of the extract proved to be promising hypocholesterolemic agents, as they were able to substantially reduce total cholesterol and LDL-C, without significantly altering triglycerides, hepatic transaminases, and alkaline phosphatase, thereby encouraging the studies with the plant H. dulcis. The groups treated with the flavonoid dihydromyricetin, although they showed a significant reduction in total cholesterol and LDL-C, and found increases in triglycerides and hepatic transaminases, which is unwanted in the context of hypercholesterolaemia