Convex Independence in Permutation Graphs

A set C of vertices of a graph is P_3-convex if every vertex outside C has at most one neighbor in C. The convex hull \sigma(A) of a set A is the smallest P_3-convex set that contains A. A set M is convexly independent if for every vertex x \in M, x \notin \sigma(M-x). We show that the maximal number of vertices that a convexly independent set in a permutation graph can have, can be computed in polynomial time

Impacto da toxina Bt sobre comunidades de leveduras epifiticas.

Resumo: O cultivo de milho (Zea mays) está entre as mais importantes culturas do mundo, e o Brasil está em terceiro lugar na produção mundial. Com o advento da biotecnologia foram desenvolvidas plantas geneticamente modificadas que expressam a toxina Bt; conferindo à planta hospedeira alto padrão de resistência a insetos evitando, assim, grandes perdas de produção. O objetivo deste trabalho foi avaliar a influência da toxina Bt presente nas folhas de milho transgênico AG8088 YG e compará-lo com as variedades não transgênicas AG8088 e BRS 1055, quanto ao numero de colônias de leveduras epifíticas apresentadas, através da técnica de diluição e espalhamento sobre superfície. A variedade transgênica de milho reduziu, significativamente, a quantidade de colônias de leveduras epifíticas, quando comparado com as demais variedades não transgênicas. Com relação à diversidade de leveduras analisadas de acordo com a morfologia das colônias, verificou-se variação inexpressiva frente à transgênica. Abstract: Corn cultivation (Zea mays) is among the most important ones of the world and Brazil holds third place in world's production. Due to biotechnology genetically-modified plants which present the Bt toxin have been developed, giving a high pattern of resistance to their host insect, thus avoiding large production losses. The objective of this study was to evaluate the influence of Bt toxin in the leaves of transgenic corn YG AG8088 on epiphytic yeast communities, through the technique of dilution and spreading on the surface. The transgenic variety reduced the amount of epiphytic yeasts colonies, compared to all other non-transgenic varieties. Comparing both treatments, it was obtained an inexpressive variation in the diversity of yeasts colonies morphology on transgenic group

Histological and mutational profile of diffuse gastric cancer: current knowledge and future challenges

Gastric cancer (GC) pathogenesis is complex and heterogeneous, reflecting morphological, molecular and genetic diversity. Diffuse gastric cancer (DGC) and intestinal gastric cancer (IGC) are the major histological types. GC may be sporadic or hereditary; sporadic GC is related to environmental and genetic low-risk factors and hereditary GC is caused by inherited high-risk mutations, so far identified only for the diffuse histotype. DGC phenotypic heterogeneity challenges the current understanding of molecular mechanisms underlying carcinogenesis. The definition of a DGC-specific mutational profile remains controversial, possibly reflecting the heterogeneity of DGC-related histological subtypes [signet-ring cell carcinoma (SRCC) and poorly cohesive carcinoma not otherwise specified (PCC-NOS)]. Indeed, DGC and DGC-related subtypes may present specific mutational profiles underlying the particularly aggressive behaviour and dismal prognosis of DGC vs IGC and PCC-NOS vs SRCC. In this systematic review, we revised the histological presentations, molecular classifications and approved therapies for gastric cancer, with a focus on DGC. We then analysed results from the most relevant studies, reporting mutational analysis data specifying mutational frequencies, and their relationship with DGC and IGC histological types, and with specific DGC subtypes (SRCC and PCC-NOS). We aimed at identifying histology-associated mutational profiles with an emphasis in DGC and its subtypes (DGC vs IGC; sporadic vs hereditary DGC; and SRCC vs PCC-NOS). We further used these mutational profiles to identify the most commonly affected molecular pathways and biological functions, and explored the clinical trials directed specifically to patients with DGC. This systematic analysis is expected to expose a DGC-specific molecular profile and shed light into potential targets for therapeutic intervention, which are currently missing.The authors acknowledge the support of the National Infrastructure ‘GenomePT’ – National Laboratory for Genome Sequencing and Analysis (POCI-01-0145-FEDER-022184). J.G-P acknowledges the support of Faculty of Medicine from University of Porto, specifically by the Doctoral Programme in Biomedicine and the Solve-RD Project (H2020-SC1-2017-Single-Stage-RTD) for his PhD fellowship. R.B-M. acknowledges the support of Institute of Biomedical Sciences Abel Salazar from University of Porto, specifically by the Doctoral Programme BiotechHealth and the FCT – Fundação para a Ciência e a Tecnologia for her PhD fellowship (ref. SFRH/BD/145132/2019). This research and its authors were funded by FEDER – Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 – Operacional Programme for Competitiveness and Internationalization (POCI), Portugal 2020, and by Portuguese funds through FCT – Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Inovação in the framework of the project ‘Institute for Research and Innovation in Health Sciences’ (POCI-01-0145-FEDER-007274). This work was also financed by the projects NORTE-01-0145-FEDER-000003 (DOCnet) and NORTE-01-0145-FEDER-000029 (CANCER) – supported by Norte Portugal Regional Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) – project POCI-01-0145-FEDER-016390 (CancelStem) and PTDC/BTM-TEC/30164/2017 (3DChroMe), funded by ERDF, POCI and FCT

THERMODYNAMIC AND THERMOPHYSICAL ASSESSMENT OF HYDROCARBONS APPLICATION IN HOUSEHOULD REFRIGERATOR

The research efforts and development in the refrigeration and air conditioning sector applied to the use of natural refrigeration fluids is not associated only with the need to preserve the environment alone, but has great importance with regard to the latent need for energy efficient equipment. With this perspective, the present study deals with the thermodynamic evaluation of the use of hydrocarbons refrigerants in household refrigeration systems that utilize HFC134a as a working fluid. A theoretical-computational analysis was developed for R134a, propane (R290) and the selected mixtures (R290/R600a 60%/40%, R290/R600a/R134a 40%/30%/30% and R600a/R290 50%/50%) in the standard refrigeration cycle ASHRAE, using the thermodynamic and thermophysical properties provided by the REFPROP 6.0 software. The results of computational simulations between the fluids were compared to find the evidence of the best alternative to HFC134a. In this sense, it was observed that the hydrocarbons reduced the levels of pressure on the condenser and evaporator, along with smaller compression tasks necessary in the system, owing to the thermo-physical properties privileged in these fluids. The use of these fluids is also proportionate to lower temperatures of compressor discharge, increasing the life of this highly valuable component of the system