The Role of the Selenoprotein Glutathione Peroxidase-1 in T Cell Activation and Differentiation

Glutathione peroxidase-1 (GPx-1) is an antioxidant enzyme that plays an important role in reducing cellular oxidative stress. The expression of GPX1 has previously been shown to be upregulated upon T cell activation in vitro and CD4+ T cells that lack GPx-1 have also been shown to preferentially differentiate into the TH1 effector subtype. These observations suggest that the activity of GPx-1 may play a role in T cell activation and differentiation. In order to determine the relationship between GPx-1 expression and T cell activation, we used a combination of in vitro and in vivo experiments to correlate GPx-1 expression with the activation marker CD44 and to address whether inhibition of GPx-1 impacts the differentiation of induced regulatory T cells. Gene expression analysis done on naïve CD4+ T cells stimulated in vitro reveals that the number of GPX1 transcripts is reduced in cells cultured in the presence of recombinant PD-L1 when compared to cells cultured in the presence of control human Ig-Fc. In contrast to these data, flow cytometry data does not reveal any significant differences between the overall abundance of intracellular GPx-1 over the course of 72 hours when comparing CD4+ T cells cultured in the presence and absence of recombinant PD-L1. Using in vivo immunization and tumor models, we demonstrate that the overall expression of GPx-1 is higher both in CD44+ T cells derived from the draining lymph node and tumor infiltrating lymphocytes when compared to CD44- T cells from each microenvironment. To assess the role of GPx-1 during activation and differentiation of naïve CD4+ T cells in vitro, we used a small molecule inhibitor of GPx-1, mercaptosuccinate (MS). Our data reveal that the addition of MS reduces the overall number of CD44+ T cells and that the presence of MS increases the oxidative state of CD4+ T cells in a dose dependent manner. These data support the role of GPx-1 as an important cytosolic and mitochondrial antioxidant enzyme. Our data also reveal that the presence of MS in cell culture media induces the expression of the transcription factor Forkhead box P3 (FoxP3) in a dose-dependent manner that is independent of the presence of TGF-β. Overall, these data suggest that GPx-1 is an important antioxidant enzyme that may play a role in regulating T cell activation and differentiation. Additionally, we provide preliminary evidence to support the idea that the redox tone of the cell may be important for fate determination. Further work is needed to assess the functionality and durability of these FoxP3 expressing T cells, and to address whether or not these cells exhibit the typical characteristics of induced regulatory T cells

Developing biodiversity assessment on a stand forest type management level in north-eastern Italy

This paper discusses a simple operative proposal, elaborated by a team of advisers to the Forestry Service of the Veneto administrative region (north-eastern Italy), concerning the definition of stand-level forest type biodiversity indicators and biodiversity oriented management provisions. Such tools are conceived to transfer biodiversity conservation understanding in current forest stand management practices. The developed assessment system is targeted to: maintenance and increase of the variability of forest landscape mosaic; conservation of species variability; creation of resource reservoirs. The following criteria and indicators are taken into consideration: spatial pattern (widespreadness, connectedness, species contagion potential), forest structure (uneven aged stands: percent of trees in three broad diameter classes; even aged stands: number of development stages and surface of each development stage), herbs/shrubs species (average, minimum and maximum number of species; average number of short-lived herb/ shrub species recorded in minimal anthropic disturbance conditions; dynamic trend in the number of herb/shrub species), bird species (average, minimum and maximum number of species); overall naturalistic quality (flora; vegetation; fauna). Such indicators are proposed as biodiversity reference standards for each forest type in the considered region: they provide practical baseline information with which forest stand management efficiency in achieving biodiversity targets can be compared

In vivo antimicrobial activity of 0.6% povidone-iodine eye drops in patients undergoing intravitreal injections: a prospective study

To investigate the antimicrobial activity of a preservative-free 0.6% povidone-iodine eye drop as an antiseptic procedure in decreasing the conjunctival bacterial load in eyes scheduled for intravitreal treatment and to compare its efficacy to the untreated fellow eye used as the control group. Prospective cohort analysis in which 208 patients received preservative-free 0.6% povidone-iodine eye drops three times a day for three days before intravitreal injection. Before and after the prophylactic treatment, a conjunctival swab was collected from both the study eye and the untreated contralateral eye, used as control. The swab was inoculated on different culture media and the colony-forming units were counted. Bacteria and fungi were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Treatment with 0.6% povidone-iodine eye drops significantly reduced the conjunctival bacterial load from baseline (p < 0.001 for blood agar and p < 0.001 for chocolate agar) with an eradication rate of 80%. The most commonly isolated pathogen at each time-point and in both groups was coagulase-negative Staphylococci, isolated in 84% of the positive cultures. The study provides evidence about the effectiveness of 0.6% povidone-iodine eye drops treatment in reducing the conjunctival bacterial load in eyes scheduled for intravitreal treatment

Sviluppo di modelli innovativi per il monitoraggio multiscala degli indicatori di servizi ecosistemici nelle foreste Mediterranee (Progetto MIMOSE).

Gli ecosistemi, attraverso le loro funzioni, forniscono un ampio range di beni e servizi, che risultano fondamentali per il benessere dell’uomo. Questi vengono complessivamente definiti Servizi Ecosistemici (SE). I SE possono essere intesi come un flusso di valori verso la società, quale risultato dello stato e della quantità del capitale naturale disponibile. Il mantenimento degli stocks del capitale naturale può permettere di prevedere i flussi futuri dei SE, assicurando quindi il benessere dell’uomo per le generazioni future. Il progetto FIRB 2012 MIMOSE è finalizzato allo sviluppo di un approccio multiscala innovativo e all’implementazione di strumenti previsionali volti al monitoraggio dei SE in habitat forestali Mediterranei. Un set di indicatori viene considerato per stimare i SE forniti dalle foreste, sviluppando strumenti integrati per il loro monitoraggio multiscala. Le attività di ricerca vertono allo sviluppo di un metodo statistico innovativo per la stima spaziale degli indicatori di SE, sulla base di dati disponibili a diversi livelli di scala spaziale. Sono realizzate mappe wall-to-wall per la fornitura dei SE, derivate da diversi domini spaziali, dal livello di scala locale sino ad aree forestali di grande estensione. I dati sugli indicatori dei SE sono forniti nel contesto di attività di campionamento già stabilite (raccolta dati a livello di aree di saggio e di popolamento), per poi applicare tecniche di spazializzazione per aggregare i dati raccolti su scala locale a livelli di scala più ampia (paesaggio, scala regionale); l’approccio utilizzato viene valutato attraverso l’applicazione di strumenti di monitoraggio in un set di aree test. Si procede quindi alla valutazione dei cambiamenti spazio-temporali nella previsione dei SE, considerando indicatori sensibili alla gestione forestale, ovvero capaci di evidenziare cambiamenti nella previsione di fornitura di beni e servizi ad opera dei sistemi forestali, in relazione a differenti scenari gestionali, con l’obiettivo di fornire indicazioni ai gestori forestali ed alle comunità locali per l’applicazione di pratiche di gestione che possano mantenere o incrementare la fornitura dei SE in un preciso contesto territoriale. Questo progetto permette di realizzare un’attenta analisi dei gaps esistenti negli attuali schemi di inventariazione in relazione alla fornitura dei SE, con l’obiettivo di dare indicazioni utili all’implementazione ed integrazione degli stessi mediante individuazione di nuovi indicatori, nell’ottica di una gestione forestale sostenibile. Le attività di ricerca in corso possono concretamente contribuire all’incorporazione dei SE nei processi decisionali connessi alla gestione dei paesaggi forestali, fornendo un’opportunità per comprendere la congruenza tra i diversi SE negli ambienti forestali

A forest typology for monitoring sustainable forest management: The case of European Forest Types

Sustainable forest management (SFM) is presently widely accepted as the overriding objective for forest policy and practice. Regional processes are in progress all over the world to develop and implement criteria and indicators of SFM. In continental Europe, a set of 35 Pan-European indicators has been endorsed under the Ministerial Conference on the Protection of Forests in Europe (MCPFE) to measure progress towards SFM in the 44 countries of the region. The formulation of seven indicators (forest area, growing stock, age structure/diameter distribution, deadwood, tree species composition, damaging agents, naturalness) requires national data to be reported by forest types. Within the vast European forest area the values taken by these indicators show a considerable range of variation, due to variable natural conditions and anthropogenic influences. Given this variability, it is very difficult to grasp the meaning of these indicators when taken out of their ecological background. The paper discusses the concepts behind, and the requirements of, a classification more soundly ecologically framed and suitable for MCPFE reporting than the three (un-informative) classes adopted so far: broadleaved forest, coniferous forest, mixed broadleaved and coniferous forest. We propose a European Forest Types scheme structured into a reasonably higher number of classes, that would improve the specificity of the indicators reported under the MCPFE process and its understanding.L'articolo è disponibile sul sito dell'editore www.tandf.co.uk/journals

Integrated forest management to prevent wildfi res under mediterranean environments

This review presents a multidisciplinary framework for integrating the ecological, regulatory, procedural and technical aspects of forest management for fi res prevention under Mediterranean environments. The aims are to: i) provide a foreground of wildfi re scenario; ii) illustrate the theoretical background of forest fuel management; iii) describe the available fuel management techniques and mechanical operations for fi re prevention in forest and wildland-urban interfaces, with exemplifi cation of case-studies; iv) allocate fi re prevention activities under the hierarchy of forest planning. The review is conceived as an outline commentary discussion targeted to professionals, technicians and government personnel involved in forestry and environmental managemen

Pentraxin 3 deficiency protects from the metabolic inflammation associated to diet-induced obesity

Aims: Low-grade chronic inflammation characterizes obesity and metabolic syndrome. Here, we aim at investigating the impact of the acute-phase protein long pentraxin 3 (PTX3) on the immune-inflammatory response occurring during diet-induced obesity. Methods and results: PTX3 deficiency in mice fed a high-fat diet for 20 weeks protects from weight gain and adipose tissue deposition in visceral and subcutaneous depots. This effect is not related to changes in glucose homeostasis and lipid metabolism but is associated with an improved immune cell phenotype in the adipose tissue of Ptx3 deficient animals, which is characterized by M2-macrophages polarization and increased angiogenesis. These findings are recapitulated in humans where carriers of a PTX3 haplotype (PTX3 h2/h2 haplotype), resulting in lower PTX3 plasma levels, presented with a reduced prevalence of obesity and decreased abdominal adiposity compared with non-carriers. Conclusion: Our results support a critical role for PTX3 in the onset of obesity by promoting inflammation and limiting adipose tissue vascularization and delineate PTX3 targeting as a valuable strategy for the treatment of adipose tissue-associated inflammatory response