Line_means

Measurement means (±S.E.) for egg retention and fertilization success for 91 DGRP lines

Egg retention counts

All raw data from the images, listing the number of counted embryos in each developmental category for every replicate measure separately and for all 91 DGRP lines

Embryo classification with Fiji (ImageJ)

The files in this folder demonstrate how the image assessment was done. It contains example microscope images, cell counter files and a step by step guide how we classified embryos

table_1.docx

Introduction<p>Rituximab (RTX) is a monoclonal antibody targeting CD20, a transmembrane protein expressed on B cells, causing B cell depletion. RTX has shown great efficacy in studies of pemphigus vulgaris, but data of pemphigoid diseases are limited.</p>Objective<p>To assess the effectiveness and safety of RTX in pemphigoid diseases.</p>Methods<p>The medical records of 28 patients with pemphigoid diseases that were treated with RTX were reviewed retrospectively. Early and late endpoints, defined according to international consensus, were disease control (DC), partial remission (PR), complete remission (CR), and relapses. Safety was measured by reported adverse events.</p>Results<p>Patients with bullous pemphigoid (n = 8), mucous membrane pemphigoid (n = 14), epidermolysis bullosa acquisita (n = 5), and linear IgA disease (n = 1) were included. Treatment with 500 mg RTX (n = 6) or 1,000 mg RTX (n = 22) was administered on days 1 and 15. Eight patients received additional 500 mg RTX at months 6 and 12. Overall, DC was achieved in 67.9%, PR in 57.1%, and CR in 21.4% of the cases. During follow-up, 66.7% patients relapsed. Repeated treatment with RTX led to remission (PR or CR) in 85.7% of the retreated cases. No significant difference in response between pemphigoid subtypes was found. IgA-dominant cases (n = 5) achieved less DC (20 vs. 81.3%; p = 0.007), less PR (20 vs. 62.5%; p = 0.149), and less CR (0 vs. 18.8%; p = 0.549) compared to IgG-dominant cases (n = 16). Five severe adverse events and three deaths were reported. One death was possibly related to RTX and one death was disease related.</p>Conclusion<p>RTX can be effective in recalcitrant IgG-dominant pemphigoid diseases, however not in those where IgA is dominant.</p