Quaternary vegetation history in Hungary

Detailed information of SSR markers used for genotyping the RIL population. (XLSX 49 kb

The length of trimmed EST sequence (cDNA length after removal of vector sequence and low quality sequences) submitted to clustering

<p><b>Copyright information:</b></p><p>Taken from "Peanut gene expression profiling in developing seeds at different reproduction stages during infection"</p><p>http://www.biomedcentral.com/1471-213X/8/12</p><p>BMC Developmental Biology 2008;8():12-12.</p><p>Published online 4 Feb 2008</p><p>PMCID:PMC2257936.</p><p></p> The number of EST within different categories of trimmed sequence length is presented on the Y-axis. The number on the X-axis represent ranges of trimmed sequence lengths (101–200, 201–300, 301–400 bp, etc, respectively)

Functional classification of peanut unique ESTs by comparison to Arabidopsis Sequencing Project functional categories

<p><b>Copyright information:</b></p><p>Taken from "Peanut gene expression profiling in developing seeds at different reproduction stages during infection"</p><p>http://www.biomedcentral.com/1471-213X/8/12</p><p>BMC Developmental Biology 2008;8():12-12.</p><p>Published online 4 Feb 2008</p><p>PMCID:PMC2257936.</p><p></p> A: functional categories of 'GT-C20' unique EST sequences; B: functional categories of 'Tifrunner' unique ESTs

Hierarchical clustering analysis of differentially expressed transcripts for 'GT-C20' and 'Tifrunner'

<p><b>Copyright information:</b></p><p>Taken from "Peanut gene expression profiling in developing seeds at different reproduction stages during infection"</p><p>http://www.biomedcentral.com/1471-213X/8/12</p><p>BMC Developmental Biology 2008;8():12-12.</p><p>Published online 4 Feb 2008</p><p>PMCID:PMC2257936.</p><p></p> TCs with R > 4 (84 in total) were used for hierarchical clustering analysis

NBS-LRR gene number and cluster number in <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>.

<p>A and B represent gene number in <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>, respectively. C and D represent cluster number in <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>, respectively.</p

Comparative analysis of NBS-LRR genes and their response to <i>Aspergillus flavus</i> in <i>Arachis</i>

<div><p>Studies have demonstrated that nucleotide-binding site–leucine-rich repeat (NBS–LRR) genes respond to pathogen attack in plants. Characterization of NBS–LRR genes in peanut is not well documented. The newly released whole genome sequences of <i>Arachis duranensis</i> and <i>Arachis ipaënsis</i> have allowed a global analysis of this important gene family in peanut to be conducted. In this study, we identified 393 (AdNBS) and 437 (AiNBS) NBS–LRR genes from <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>, respectively, using bioinformatics approaches. Full-length sequences of 278 AdNBS and 303 AiNBS were identified. Fifty-one orthologous, four AdNBS paralogous, and six AiNBS paralogous gene pairs were predicted. All paralogous gene pairs were located in the same chromosomes, indicating that tandem duplication was the most likely mechanism forming these paralogs. The paralogs mainly underwent purifying selection, but most LRR 8 domains underwent positive selection. More gene clusters were found in <i>A</i>. <i>ipaënsis</i> than in <i>A</i>. <i>duranensis</i>, possibly owing to tandem duplication events occurring more frequently in <i>A</i>. <i>ipaënsis</i>. The expression profile of NBS–LRR genes was different between <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>hypogaea</i> after <i>Aspergillus flavus</i> infection. The up-regulated expression of NBS–LRR in <i>A</i>. <i>duranensis</i> was continuous, while these genes responded to the pathogen temporally in <i>A</i>. <i>hypogaea</i>.</p></div

Comparison of K_a/K_s values among NBS-LRR sequence, NBS and LRR regions.

<p>A, B and C represent NBS-LRR sequence, NBS and LRR regions, respectively.</p

Chromosomal location and homologous gene relationship of NBS-LRR genes from <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>.

<p>The letters and numbers outside the circle represent species and chromosomes, respectively. A and B represent <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>, respectively.</p

Expression of NBS-LRR genes from <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>hypogaea</i> after <i>A</i>. <i>flavus</i> infection.

<p>The Y-axis indicates the relative expression level; X-axis indicates days of <i>A</i>. <i>flavus</i> infection. The standard errors are plotted using vertical lines.</p

Phylogenetic tree of NBS-LRR from <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i>.

<p>The phylogenetic tree was generated using CNL and TNL full-length proteins from <i>A</i>. <i>duranensis</i> and <i>A</i>. <i>ipaënsis</i> using MEGA 6.0 by the maximum likelihood (ML) with Jones-Taylor-Thornton model based on 1,000 bootstrap replicates.</p