Analysis of the operational status of the three-level referral system for urologic ultrasound screening and risk factors for renal pelvic dilatation in high-risk children

BackgroundCongenital Anomalies of the Kidney and Urinary Tract (CAKUT) are the primary cause of end-stage renal disease in children, early diagnosis and treatment can significantly improve the kidney function. Among CAKUT, renal pelvis dilatation (RPD) due to various causes has the highest detection rate, which can be detected early by postnatal ultrasound screening. Since 2010, the Children's Hospital of Fudan University (CHFU), together with the Minhang District Maternal and Child Health Hospital (MCH) and Community Health Centres (CHCs) of Minhang District has created a three-level referral system for urological ultrasound screening. This study aims to describe the operation of a three-level referral system for ultrasound screening of CAKUT and to select risk factors of RPD in high-risk children.MethodsThe operation of the three-level referral system was assessed by analyzing the screening volume, screening rate, referral rate, and follow-up rate; risk factors of RPD in high-risk children were selected by chi-square test and multivariate logistic regression.ResultsA total of 16,468 high-risk children were screened in ten years, and the screening volume was maintained at about 1,500 cases per year; the screening rate showed a linear increase, from 36.8% in 2010 to 98.2% in 2019; the referral rate from the CHCs to the MCH was 89.9% significantly higher after 2015 than that of 84.7% from 2010 to 2015; the follow-up rate after 2015 was 71.0% significantly higher than that of 46.3% from 2010 to 2015. Multivariate logistic regression analysis showed that the risk of RPD was 1.966 times higher in males than in females, and the risk of moderate to severe RPD was 2.570 times higher in males than in females; the risk of RPD in preterm children was 1.228 times higher than that of full-term children; and the risk of RPD was 1.218 times higher in twins than in singles.ConclusionsThe screening volume of the three-level referral system has remained stable over a decade, with significantly higher screening, referral, and follow-up rates. Males, preterm, and twins are risk factors of RPD in high-risk children; males are also risk factors for moderate to severe RPD in high-risk children

Identification of pivotal genes and regulatory networks associated with atherosclerotic carotid artery stenosis based on comprehensive bioinformatics analysis and machine learning

Objective:Bioinformatics methods were applied to investigate the pivotal genes and regulatory networks associated with atherosclerotic carotid artery stenosis (ACAS) and provide new insights for the treatment of this disease.Methods:The study utilized five ACAS datasets (GSE100927, GSE11782, GESE28829, GSE41571, and GSE43292) downloaded from the NCBI GEO database. The first four datasets were combined as the training set (n = 99), while GSE43292 (n = 64) was used as the validation set. Difference analysis and functional enrichment analysis were then performed on the training set. The pathogenic targets of ACAS were screened by protein-protein interaction networks and MCODE analyses, combined with three machine learning algorithms. The results were next verified by analysis of inter-group differences and ROC curve analysis. Next, immune-related function and immune cell correlation analyses were performed, and plaques of human ACAS were applied to verify the results via immunohistochemistry (IH) and immunofluorescence (IF). Finally, the competing endogenous RNAs (ceRNA) and transcription factors (TFs) regulatory networks of the characterized genes were constructed.Results:A total of 177 differentially expressed genes were identified, including 67 genes downregulated and 110 genes upregulated. Gene set enrichment analysis revealed that five pathways were active in the experimental group, including xenograft rejection, autoimmune thyroid disease, graft-versus-host disease, leishmaniasis infection, and lysosomes. Four key genes were identified, with C3AR1 being upregulated and FBLN5, PPP1R12A, and TPM1 being downregulated. The analysis of inter-group differences demonstrated that the four characterized genes were differentially expressed in both the control and experimental groups. The ROC analysis showed that they had high AUC values in both the training and validation sets. Therefore, a predictive ACAS patient nomogram model based on the screened genes was established. Correlation analysis revealed a positive correlation between C3AR1 expression and neutrophils, which was further validated in IH and IF. One or multiple lncRNAs may compete with the characterized genes for binding miRNAs. Additionally, each characterized gene interacts with multiple TFs.Conclusion:Four pivotal genes were screened, and relevant ceRNA and TFs were predicted. These molecules may exert a crucial role in ACAS and serve as potential biomarkers and therapeutic targets

Robust cross-linked Na3V2(PO4)2F3 full sodium-ion batteries

Sodium-ion batteries (SIBs) have rapidly risen to the forefront of energy storage systems as a promising supplementary for Lithium-ion batteries (LIBs). Na3V2(PO4)2F3 (NVPF) as a common cathode of SIBs, features the merits of high operating voltage, small volume change and favorable specific energy density. However, it suffers from poor cycling stability and rate performance induced by its low intrinsic conductivity. Herein, we propose an ingenious strategy targeting superior SIBs through cross-linked NVPF with multi-dimensional nanocarbon frameworks composed of amorphous carbon and carbon nanotubes (NVPF@C@CNTs). This rational design ensures favorable particle size for shortened sodium ion transmission pathway as well as improved electronic transfer network, thus leading to enhanced charge transfer kinetics and superior cycling stability. Benefited from this unique structure, significantly improved electrochemical properties are obtained, including high specific capacity (126.9 mAh g−1 at 1 C, 1 C = 128 mA g−1) and remarkably improved long-term cycling stability with 93.9% capacity retention after 1000 cycles at 20 C. The energy density of 286.8 Wh kg−1 can be reached for full cells with hard carbon as anode (NVPF@C@CNTs//HC). Additionally, the electrochemical performance of the full cell at high temperature is also investigated (95.3 mAh g−1 after 100 cycles at 1 C at 50 oC). Such nanoscale dual-carbon networks engineering and thorough discussion of ion diffusion kinetics might make contributions to accelerating the process of phosphate cathodes in SIBs for large-scale energy storages

Quantitatively Computing the Influence of Vegetation Changes on Surface Discharge in the Middle-Upper Reaches of the Huaihe River, China

Changes in meteorology, hydrology, and vegetation will have significant impacts on the ecological environment of a basin, and the middle-upper reach of Huaihe River (MUHR) is one of the key regions for vegetation restoration in China. However, less studies have quantitatively accounted for the contribution of vegetation changes to land surface discharge in the MUHR. To quantitatively evaluate the influence of vegetation changes on land surface discharge in the MUHR, the Bernaola–Galavan (B–G) segmentation algorithm was utilized to recognize the mutation year of the Normalized Difference Vegetation Index (NDVI) time sequence data. Next, the functional relationship between the underlying surface parameter and the NDVI was quantitatively analyzed, and an adjusted Budyko formula was constructed. Finally, the effects of vegetation changes, climate factors, and mankind activities on the surface discharge in the MUHR were computed using the adjusted Budyko formula and elastic coefficient method. The results showed the following: (1) the surface runoff and precipitation from 1982 to 2015 in the MUHR presented a falling trend, yet the NDVI and potential evaporation presented an upward trend; (2) 2004 was the mutation year of the NDVI time series data, and the underlying surface parameter showed a significant linear regression relationship with the NDVI (p < 0.05); (3) the vegetation variation played a major role in the runoff variation during the changing period (2005–2015) in the MUHR. Precipitation, potential evaporation, and human activities accounted for −0.32%, −15.11%, and 18.24% of the surface runoff variation, respectively

Using personal comfort systems during the post-heating season in a cold climate: A field study in offices

A field investigation of the indoor thermal environment and comfort in a typical office building in Qingdao was conducted. During the investigation, the indoor and outdoor environment physical parameters, such as air temperature and relative humidity (RH), were recorded in real-time. The occupants’ thermal responses, basic personal information, and adaptive behaviors were collected. The results showed that the indoor air temperature was from 18.8 °C to 21.6 °C, which was 2–3 °C lower than that during the heating season. Occupants employed heating personal comfort systems (HPCSs), such as electric heaters, air heaters, and leg radiators, to maintain thermal comfort. Based on their requirements for HPCSs, the occupants were divided into three groups-those without HPCS and did not prefer to use (Group A), those with HPCS (Group B), and those without HPCS but preferred to use (Group C). The results showed that the legs and feet thermal sensation of Group B was significantly higher than that of Group C, which made Group B more comfortable. The self-evaluated working efficiency of Group B was slightly higher than that of Group C. Notably, working efficiency was positively related to the overall thermal comfort vote (TCV)

Quantitatively Computing the Influence of Vegetation Changes on Surface Discharge in the Middle-Upper Reaches of the Huaihe River, China

Changes in meteorology, hydrology, and vegetation will have significant impacts on the ecological environment of a basin, and the middle-upper reach of Huaihe River (MUHR) is one of the key regions for vegetation restoration in China. However, less studies have quantitatively accounted for the contribution of vegetation changes to land surface discharge in the MUHR. To quantitatively evaluate the influence of vegetation changes on land surface discharge in the MUHR, the Bernaola&ndash;Galavan (B&ndash;G) segmentation algorithm was utilized to recognize the mutation year of the Normalized Difference Vegetation Index (NDVI) time sequence data. Next, the functional relationship between the underlying surface parameter and the NDVI was quantitatively analyzed, and an adjusted Budyko formula was constructed. Finally, the effects of vegetation changes, climate factors, and mankind activities on the surface discharge in the MUHR were computed using the adjusted Budyko formula and elastic coefficient method. The results showed the following: (1) the surface runoff and precipitation from 1982 to 2015 in the MUHR presented a falling trend, yet the NDVI and potential evaporation presented an upward trend; (2) 2004 was the mutation year of the NDVI time series data, and the underlying surface parameter showed a significant linear regression relationship with the NDVI (p &lt; 0.05); (3) the vegetation variation played a major role in the runoff variation during the changing period (2005&ndash;2015) in the MUHR. Precipitation, potential evaporation, and human activities accounted for &minus;0.32%, &minus;15.11%, and 18.24% of the surface runoff variation, respectively

Knockdown of Rab21 inhibits proliferation and induces apoptosis in human glioma cells

Abstract Background Gliomas are commonly malignant tumors that arise in the human central nervous system and have a low overall five-year survival rate. Previous studies reported that several members of Rab GTPase family are involved in the development of glioma, and abnormal expression of Rab small GTPases is known to cause aberrant tumor cell behavior. In this study, we characterized the roles of Rab21 (Rab GTPase 21), a member of Rab GTPase family, in glioma cells. Methods The study involved downregulation of Rab21 in two glioma cell lines (T98G and U87) through transfection with specific-siRNA. Experiments using the MTT assay, cell cycle analysis, apoptosis assay, real-time PCR and western blot were performed to establish the expression levels of related genes. Results The results show that downregulation of Rab21 can significantly inhibit cell growth and remarkably induce cell apoptosis in T98G and U87 cell lines. Silencing Rab21 resulted in significantly increased expression of apoptosis-related proteins (caspase7, Bim and Bax) in glioma cells. Conclusions We inferred that Rab21 silencing can induce apoptosis and inhibit proliferation in human glioma cells, indicating that Rab21 might act as an oncogene and serve as a novel target for glioma therapy

Minnelide combined with anti-ANGPTL3-FLD monoclonal antibody completely protects mice with adriamycin nephropathy by promoting autophagy and inhibiting apoptosis

Abstract Minimal change disease (MCD) is the common type of nephrotic syndrome (NS) in children. Currently, there is an urgent need to explore new treatments because of the significant side effects of long-term use of glucocorticoids and immunosuppressive drugs and the failure to reduce proteinuria in some patients. Angiopoietin-like protein 3 (Angptl3) is an essential target of NS, and anti-ANGPTL3-FLD monoclonal antibody (mAb) significantly reduces proteinuria in mice with adriamycin nephropathy (AN). However, some proteinuria is persistent. Minnelide, a water-soluble prodrug of triptolide, has been used for the treatment of glomerular disease. Therefore, the present study aimed to investigate whether minnelide combined with mAb could further protect mice with AN and the underlying mechanisms. 8-week-old C57BL/6 female mice were injected with 25 mg/kg of Adriamycin (ADR) by tail vein to establish the AN model. A dose of 200 μg/kg of minnelide or 20 mg/kg of mAb was administered intraperitoneally for the treatment. In vitro, the podocytes were treated with 0.4 μg/mL of ADR for 24 h to induce podocyte injury, and pretreatment with 10 ng/mL of triptolide for 30 min or 100 ng/mL of mAb for 1 h before ADR exposure was used to treat. The results showed that minnelide combined with mAb almost completely ameliorates proteinuria and restores the ultrastructure of the podocytes in mice with AN. In addition, minnelide combined with mAb restores the distribution of Nephrin, Podocin, and CD2AP and reduces the level of inflammatory factors in mice with AN. Mechanistically, minnelide combined with mAb could further alleviate apoptosis and promote autophagy in mice with AN by inhibiting the mTOR signaling pathway. In vitro, triptolide combined with mAb increases the expression of Nephrin, Podocin, and CD2AP, alleviates apoptosis, and promotes autophagy. Overall, minnelide combined with mAb completely protects the mice with AN by promoting autophagy and inhibiting apoptosis

Multimedia in Home Networking

The basic challenge of home networking is to connect devices anywhere in the house in a way that is simple, reliable, inexpensive and secure. Increasing interaction with computers and other electronic &amp; electrical devices in our day to day life has fueled the demand for an increased performance of home networks. With multimedia becoming an integral part of our lives, establishing a reliable home network to support multimedia is of great interest. Starting with the discussion of various home networking technologies and their QoS, middleware considerations and network security, this paper specifically deals with PHY and MAC layer description of home networks that support multimedia

Upconversion Luminescence and Discussion of Sensitivity Improvement for Optical Temperature Sensing Application

Upconversion (UC) based luminescent materials have promising applications in noncontact temperature sensors. How to improve the sensitivity is one main object at present. This work presented several strategies for optical temperature sensing based on UC spectra of the Y₂WO₆:Yb³⁺-Er³⁺/Ho³⁺/Tm³⁺ phosphors. The improvement for the relative (<i>S</i>_R) and absolute (<i>S</i>_A) sensitivities were discussed by using a fluorescence intensity ratio technique. It includes thermally coupled levels (TCLs) and non-TCLs. It was proposed that a piecewise expression could be employed to achieve high <i>S</i>_A value for TCLs. However, improving the <i>S</i>_R value is limited for TCLs. With regard to the non-TCLs, <i>S</i>_R and <i>S</i>_A are not restricted, but not easy to be improved synchronously. On the other hand, the morphology and UC spectra of the samples were also studied. The above investigation could be instructive to develop new luminescent materials with high sensitivity