2014-schematic diagram

A schematic diagram of damage pattern after irraditio

2014-RBC morphology

containing the RBC morphology pic data after irradiatio

2014-RBC size changes data

RBC size changes data including in the physical parameters of the irradiation, such as Length, Width,Perimeter,ROI area and Volume

2014--AFM measurment data

AFM measurment data contain Youngs modulus data groups,c ions histogram-occurrence data,x rays histogram- occurrence data,P correlation analysis data,Elasticity modulus-Scatter dots and Youngs modulus- dose-time relationship after irradiation

2014-Tip calibration

Tip calibration pic dat

Measurement of the changes of erythrocytes shape by induced of ¹²C⁶⁺ ions and X-rays radiation in <i>Mesocricetus auratus.</i>

<p><b>Note</b>, Measurement of the morphological differences of erythrocytes had indicated the obvious impact of ¹²C⁶⁺ ions or X-rays radiation with Image J software. And these general varies mainly includes the physical and chemical parameters of the erythrocyte, such as length, width, perimeter, thickness, ROI area and volume (of average value). Because of the average thickness of erythrocytes in mammals is around in the range of 1.0–2.5 µm, and using an optical microscope in the experiment, so as to calculate the volume, we taken the average thickness value of 2.0 µm to obtain the relative size of erythrocytes. And in the list of <b>Tab</b>., ^▴ Black triangle symbols significant statistical significance compared with the control group in carbon ion radiation (<i>p<0.05</i>), but ^▴▴ double triangle line represent the differences results by inducing X-rays (<i>p<0.05</i>). (In detail, for length, Control <i>vs</i> C ions-2Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> C ions-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> C ions-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-12Gy, p = 0.000, <i>p<0.05;</i> for width, Control <i>vs</i> C ions-2Gy, p = 0.479, <i>p>0.05;</i> Control <i>vs</i> C ions-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> C ions-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-12Gy, p = 0.000, <i>p<0.05;</i> for perimeter, Control <i>vs</i> C ions-2Gy, p = 0.002, <i>p<0.05;</i> Control <i>vs</i> C ions-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> C ions-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-4Gy, p = 0.027, <i>p<0.05;</i> Control <i>vs</i> X rays-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-12Gy, p = 0.000, <i>p<0.05;</i> for volume, Control <i>vs</i> C ions-2Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> C ions-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> C ions-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-4Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-6Gy, p = 0.000, <i>p<0.05;</i> Control <i>vs</i> X rays-12Gy, p = 0.000, <i>p<0.05.</i>).</p><p>Measurement of the changes of erythrocytes shape by induced of ¹²C⁶⁺ ions and X-rays radiation in <i>Mesocricetus auratus.</i></p

Histograms of erythrocytes elasticity modulus distributions induced by C ions or X-rays exposed for 3, 14 and 28 days.

<p>The bar graph (<b>A</b>), (<b>B</b>) and (<b>C</b>) represented control erythrocytes (0 Gy), 3d, 14d and 28d, respectively; the bar graph (<b>D</b>), (<b>E</b>) and (<b>F</b>) represented carbon ions 2 Gy, 3d, 14d and 28d, respectively; the bar graph (<b>G</b>), (<b>H</b>) and (<b>I</b>) represented carbon ions 4 Gy, 3d, 14d and 28d, respectively; the bar graph (<b>J</b>), (<b>K</b>) and (<b>L</b>) represented carbon ions 6 Gy, 3d, 14d and 28d, respectively. But the bar graph (<b>M</b>), (<b>N</b>) and (<b>O</b>) showed X-rays 4 Gy, 3d, 14d and 28d, respectively; the bar graph <b>(P</b>), (<b>Q</b>) and (<b>R</b>) showed X-rays 6 Gy, 3d, 14d and 28d; the bar graph (<b>S</b>), (<b>T</b>) and (<b>U</b>) showed X-rays 12 Gy, 3d, 14d and 28d, respectively.</p

Effects of radiation on micromorphology in erythrocytes membrane.

<p>(<b>A–L</b>) Surface imaging of erythrocytes induced by carbon ion beams and X-rays radiation using biological type atomic force microscopy; and the graph <b>A</b>, <b>B</b> and <b>C</b> showed respectively for the Height measured image, Deflection image and 3D image at scan scale of 50 µm (Fast)×50 µm bar (Slow), [for <b>A</b>:(1) Control; (2) C ions 2Gy; (3) C ions 4Gy; (4) C ions 6Gy; (5) X-rays 4Gy; (6) X-rays 6Gy; (7) X-rays 12Gy; for <b>B</b>: (1–1) Control; (2–1) C ions 2Gy; (3–1) C ions 4Gy; (4–1) C ions 6Gy; (5–1) X-rays 4Gy; (6–1) X-rays 6Gy; (7–1) X-rays 12Gy; for <b>C</b>: (1–11) Control; (2–11) C ions 2Gy; (3–11) C ions 4Gy; (4–11) C ions 6Gy; (5–11) X-rays 4Gy; (6–11) X-rays 6Gy; (7–11) X-rays 12Gy]. But the graph <b>D</b>–<b>F</b> showed respectively for the Height measured image, Deflection image and 3D image at scan scale of 25 µm (Fast) ×25 µm bar (Slow); the graph <b>G</b>–<b>I</b> showed respectively for the Height measured image, Deflection image and 3D image at scan scale of 12.5 µm (Fast) ×12.5 µm bar (Slow); the graph <b>J</b>–<b>L</b> showed respectively for the Height measured image, Deflection image and 3D image at scan scale of 5 µm (Fast) × 5 µm bar (Slow). In addition, for a smaller scan scale was the same grouping as 50 µm (Fast) × 50 µm bar (Slow) bar. Here, the schematic showed different doses and type radiation for the influence of micromorphology of erythrocytes, and this fine change was captured by AFM.</p

Photographs of analysis the changes of gross morphology of erythrocytes in blood smear at 3d after whole-body exposure to¹²C⁶⁺ ions or X-rays (by 100×oil immersion observation).

<p>Panel <b>a</b>: Non-irradiated control group erythrocytes (0 Gy); Panel <b>b</b>–<b>d</b>: erythrocytes of carbon ions radiation groups (Radiation dose: 2 Gy, 4 Gy and 6 Gy); and Panel <b>e</b>–<b>g</b>: erythrocytes of X-rays radiation groups (Radiation dose: 4 Gy, 6 Gy and 12 Gy). Black arrows indicate the morphological changes of erythrocytes. The scale bar is 10 µm.</p

The change of elasticity modules of erythrocytes induced by C ions or X-rays radiation.

<p>And the groups displayed as a function of the dependent manner on dosage (<b>A</b> and <b>B</b>) and time (<b>C</b> and <b>D</b>). Error bars represent half-widths of the normal distributions fitted to the respective histograms.</p