Review of reactive power dispatch strategies for loss minimization in a DFIG-based wind farm

This paper reviews and compares the performance of reactive power dispatch strategies for the loss minimization of Doubly Fed Induction Generator (DFIG)-based Wind Farms (WFs). Twelve possible combinations of three WF level reactive power dispatch strategies and four Wind Turbine (WT) level reactive power control strategies are investigated. All of the combined strategies are formulated based on the comprehensive loss models of WFs, including the loss models of DFIGs, converters, filters, transformers, and cables of the collection system. Optimization problems are solved by a Modified Particle Swarm Optimization (MPSO) algorithm. The effectiveness of these strategies is evaluated by simulations on a carefully designed WF under a series of cases with different wind speeds and reactive power requirements of the WF. The wind speed at each WT inside the WF is calculated using the Jensen wake model. The results show that the best reactive power dispatch strategy for loss minimization comes when the WF level strategy and WT level control are coordinated and the losses from each device in the WF are considered in the objective

T cell immunity rather than antibody mediates cross-protection against Zika virus infection conferred by a live attenuated Japanese encephalitis SA14-14-2 vaccine.

Zika virus (ZIKV) and Japanese encephalitis virus (JEV) are closely related to mosquito-borne flaviviruses. Japanese encephalitis (JE) vaccine SA14-14-2 has been in the Chinese national Expanded Program on Immunization since 2007. The recent recognition of severe disease syndromes associated with ZIKV, and the identification of ZIKV from mosquitoes in China, prompts an urgent need to investigate the potential interaction between the two. In this study, we showed that SA14-14-2 is protective against ZIKV infection in mice. JE vaccine SA14-14-2 triggered both Th1 and Th2 cross-reactive immune responses to ZIKV; however, it was cellular immunity that predominantly mediated cross-protection against ZIKV infection. Passive transfer of immune sera did not result in significant cross-protection but did mediate antibody-dependent enhancement in vitro, though this did not have an adverse impact on survival. This study suggests that the SA14-14-2 vaccine can protect against ZIKV through a cross-reactive T cell response. This is vital information in terms of ZIKV prevention or precaution in those ZIKV-affected regions where JEV circulates or SA14-14-2 is in widespread use, and opens a promising avenue to develop a novel bivalent vaccine against both ZIKV and JEV. KEY POINTS: • JEV SA14-14-2 vaccine conferred cross-protection against ZIKV challenge in mice. • T cell immunity rather than antibody mediated the cross-protection. • It provides important information in terms of ZIKV prevention or precaution

Genome-wide CRISPR/Cas9 screening for drug resistance in tumors

Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) screening is a simple screening method for locating loci under specific conditions, and it has been utilized in tumor drug resistance research for finding potential drug resistance-associated genes. This screening strategy has significant implications for further treatment of malignancies with acquired drug resistance. In recent years, studies involving genome-wide CRISPR/Cas9 screening have gradually increased. Here we review the recent application of genome-wide CRISPR/Cas9 screening for drug resistance, involving mitogen-activated protein kinase (MAPK) pathway inhibitors, poly (ADP-ribose) polymerase inhibitors (PARPi), alkylating agents, mitotic inhibitors, antimetabolites, immune checkpoint inhibitors (ICIs), and cyclin-dependent kinase inhibitors (CDKI). We summarize drug resistance pathways such as the KEAP1/Nrf2 pathway MAPK pathway, and NF-κB pathway. Also, we analyze the limitations and conditions for the application of genome-wide CRISPR/Cas9 screening techniques

Organic Fluorescent Compounds that Display Efficient Aggregation-Induced Emission Enhancement and Intramolecular Charge Transfer

A series of symmetric sulfone-linked organic fluorescent compounds (1a–c) was synthesized and characterized. V-shaped 1a–c were designed as aggregate of intramolecular charge transfer (ICT) and aggregation-induced emission enhancement (AIEE) processes. The 1a–c emitted intense blue violet lights in normal solvents. A large red shift of the emission wavelength and dramatic decrease of emission efficiency occurred with increasing solvent polarity. The 1a–c will function well as electron transport and blue light-emitting materials through theoretical calculations

Cross-Reactive Immunity among Five Medically Important Mosquito-Borne Flaviviruses Related to Human Diseases

Flaviviruses cause a spectrum of potentially severe diseases. Most flaviviruses are transmitted by mosquitoes or ticks and are widely distributed all over the world. Among them, several mosquito-borne flaviviruses are co-epidemic, and the similarity of their antigenicity creates abundant cross-reactive immune responses which complicate their prevention and control. At present, only effective vaccines against yellow fever and Japanese encephalitis have been used clinically, while the optimal vaccines against other flavivirus diseases are still under development. The antibody-dependent enhancement generated by cross-reactive immune responses against different serotypes of dengue virus makes the development of the dengue fever vaccine a bottleneck. It has been proposed that the cross-reactive immunity elicited by prior infection of mosquito-borne flavivirus could also affect the outcome of the subsequent infection of heterologous flavivirus. In this review, we focused on five medically important flaviviruses, and rearranged and recapitulated their cross-reactive immunity in detail from the perspectives of serological experiments in vitro, animal experiments in vivo, and human cohort studies. We look forward to providing references and new insights for the research of flavivirus vaccines and specific prevention

Influence of Scanning Speed on Microstructure and Properties of Laser Cladded Fe-Based Amorphous Coatings

Fe-based amorphous alloys with excellent mechanical properties are suitable for preparing wear resistant coatings by laser cladding. In this study, a novel Fe-based amorphous coating was prepared by laser cladding on 3Cr13 stainless steel substrates. The influence of scanning speeds on the microstructures and properties of the coatings was investigated. The microstructure compositions and phases were analyzed by scanning electron microscope, electron probe microanalyzer, and x-ray diffraction respectively. Results showed that the microstructures of the coatings changed significantly with the increase of scanning speeds. For a scanning speed of 6 mm/s, the cladding layer was a mixture of amorphous and crystalline regions. For a scanning speed of 8 mm/s, the cladding layer was mainly composed of block grain structures. For a scanning speed of 10 mm/s, the cladding layer was composed entirely of dendrites. Different dilution rates at the bonding zones were the main reasons for the microstructure change for different claddings. For all three scanning speeds, the coatings had higher hardness and wear resistance when compared with the substrate; as the scanning speed increased, the hardness and wear resistance of the coatings gradually decreased due to the change in microstructure