6 research outputs found
Regulatory Network Analyses Reveal Genome-Wide Potentiation of LIF Signaling by Glucocorticoids and Define an Innate Cell Defense Response
While the hypothalamo-pituitary-adrenal axis (HPA) activates a general stress response by increasing glucocorticoid (Gc) synthesis, biological stress resulting from infections triggers the inflammatory response through production of cytokines. The pituitary gland integrates some of these signals by responding to the pro-inflammatory cytokines IL6 and LIF and to a negative Gc feedback loop. The present work used whole-genome approaches to define the LIF/STAT3 regulatory network and to delineate cross-talk between this pathway and Gc action. Genome-wide ChIP-chip identified 3,449 STAT3 binding sites, whereas 2,396 genes regulated by LIF and/or Gc were found by expression profiling. Surprisingly, LIF on its own changed expression of only 85 genes but the joint action of LIF and Gc potentiated the expression of more than a thousand genes. Accordingly, activation of both LIF and Gc pathways also potentiated STAT3 and GR recruitment to many STAT3 targets. Our analyses revealed an unexpected gene cluster that requires both stimuli for delayed activation; 83% of the genes in this cluster are involved in different cell defense mechanisms. Thus, stressors that trigger both general stress and inflammatory responses lead to activation of a stereotypic innate cellular defense response
A Serum Shock Induces Circadian Gene Expression in Mammalian Tissue Culture Cells
AbstractThe treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals. Oscillating genes include rper1 and rper2(rat homologs of the Drosophila clock gene period), and the genes encoding the transcription factors Rev-Erbα, DBP, and TEF. In rat-1 fibroblasts, up to three consecutive daily oscillations with an average period length of 22.5 hr could be recorded. The temporal sequence of the various mRNA accumulation cycles is the same in cultured cells and in vivo. The serum shock of rat-1 fibroblasts also results in a transient stimulation of c-fos and rper expression and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus
Anatomical terminology of the internal nose and paranasal sinuses: cross-cultural adaptation to Portuguese
Introduction: Functional endonasal endoscopic surgery is a frequent surgical procedure among otorhinolaryngologists. In 2014, the European Society of Rhinology published the âEuropean Position Paper on the Anatomical Terminology of the Internal Nose and Paranasal Sinusesâ, aiming to unify the terms in the English language. We do not yet have a unified terminology in the Portuguese language. Objective: Transcultural adaptation of the anatomical terms of the nose and paranasal cavities of the âEuropean Anatomical Terminology of the Internal Nose and Paranasal Sinusesâ to Portuguese. Methods: A group of rhinologists from diverse parts of Brazil, all experienced in endoscopic endonasal surgery, was invited to participate in the creation of this position paper on the anatomical terms of the nose and paranasal sinuses in the Portuguese language according to the methodology adapted from that previously described by Rudmik and Smith. Results: The results of this document were generated based on the agreement of the majority of the participants according to the most popular suggestions among the rhinologists. A cross-cultural adaptation of the sinonasal anatomical terminology was consolidated. We suggest the terms âinferior turbinateâ, ânasal septumâ, â(bone/cartilaginous) part of the nasal septumâ, â(middle/inferior) nasal meatusâ, âfrontal sinus drainage pathwayâ, âfrontal recessâ and âuncinate processâ be standardized. Conclusion: We have consolidated a Portuguese version of the European Anatomical Terminology of the Internal Nose and Paranasal Sinuses, which will help in the publication of technical announcements, scientific publications and the teaching of the internal anatomical terms of the nose and paranasal sinuses in Brazil. Resumo: Introdução: A cirurgia endoscĂłpica funcional endonasal Ă© um procedimento cirĂșrgico frequente entre os otorrinolaringologistas. Em 2014, a Sociedade Europeia de Rinologia publicou o âDocumento Europeu para Posicionamento sobre a Terminologia AnatĂŽmica Interna do Nariz e das Cavidades Paranasaisâ com o objetivo de unificar os termos na lĂngua inglesa. Ainda nĂŁo dispomos de uma terminologia unificada na lĂngua portuguesa. Objetivo: Adaptação transcultural dos termos anatĂŽmicos do nariz e das cavidades paranasais para o portuguĂȘs da âEuropean Anatomical Terminology of the Internal Nose and Paranasal Sinusesâ. MĂ©todo: Um grupo de rinologistas de todo o Brasil, com experiĂȘncia em cirurgia endoscĂłpica endonasal, foi convidado a participar da elaboração desse posicionamento sobre os termos anatĂŽmicos do nariz e das cavidades paranasais para o portuguĂȘs conforme metodologia adaptada da previamente descrita por Rudmik e Smith. Resultados: Os resultados desse documento foram gerados a partir da concordĂąncia da maioria dos participantes conforme as sugestĂ”es mais populares entre os rinologistas. Uma adaptação transcultural da terminologia anatĂŽmica nasossinusal foi consolidada. Sugerimos que se busque uniformizar termos como âconcha inferiorâ, âsepto nasalâ, âporção (Ăłssea/cartilagionasa) do septo nasalâ, âmeato (mĂ©dio/ inferior) nasalâ, âvia da drenagem do seio frontalâ,ârecesso frontalâ e âprocesso uncinadoâ. ConclusĂŁo: Consolidamos uma versĂŁo adaptada em portuguĂȘs da âEuropean Anatomical Terminology of the Internal Nose and Paranasal Sinusesâ que auxiliarĂĄ a publicação de comunicados tĂ©cnicos, publicaçÔes cientĂficas e o ensino dos termos anatĂŽmicos internos do nariz e das cavidades paranasais no Brasil. Keywords: Cross-cultural adaptation, Anatomy, Nose, Paranasal sinus, Consensus, Palavras-chave: Adaptação transcultural, Anatomia, Nariz, Cavidades paranasais, Consens