Modulation of the intestinal microbiota and expression of gene of senegalese sole (Solea senegalensis, Kaup 1858) after dietary administration of oxytetracycline.

Oxitetracycline and probiotics (Pdp11) administration to sole juveniles showed important changes in the species composition of intestinal microbiota that established a relationship between these species and gene expression

Changes in intestinal microbiota and gene expression of immune system parameters caused by dietary administration of alginate and Shewanella putrefaciens Pdp11 in Senegalese sole (Solea senegalensis Kaup, 1858) specimens

Dietary administration of sodium alginate single or mixed with Pdp11 modulated the intestinal microbiota diversity of sole juveniles. Both treatments promoted a change in the species present in the gut in comparison with control group. In addition, oral administration of alginate promoted the appearance of an intestinal microbiota highly influenced by the prebiotic presence. Synbiotic administration of alginate with Pdp11 promoted a more pronounced decreasing in the similarity index and in the species richness. Both treatments modulated the expression of genes related with immune system functions

Use of in vivo-induced antigen technology to identify bacterial genes expressed during solea senegalensis infection with Photobacterium damselae subsp. piscicida

The marine fish pathogen Photobacterium damselae subsp. piscicida (Phdp) is responsible for important outbreaks affecting several fish species including flatfish Solea senegalensis. The aim of this work was to identify in vivo-induced expressed immunogenic proteins using pooled sera from fish that have experienced photobacteriosis. In vivo induced immunogenic proteins included inosine-5'-monophosphate dehydrogenase (Impdh) and alkyl hydroperoxide reductase (AhpC), two proteins involved in peptide synthesis: serine hydroxymethyl transferase (Shmt) and alanyl-tRNA synthetase (AlaRS) and the non-ribosomal peptide synthetase involved in the synthesis of the siderophore piscibactin (Irp2)

Relación entre la microbiota intestinal y la composición de ácidos grasos tras la administración del probiótico Shewanella putrefaciens Pdp11 bioencapsulado en Artemia en ejemplares larvarios de S. senegalensis

Estudios previos confirmaron que un pulso corto (10-30 dpe) del probiótico S. putrefaciens Pdp11 (2.5x10 7 cfu mL-1) bioencapsulado en Artemia y administrado a ejemplares larvarios de S. senegalensis incrementaron significativamente los niveles de lípidos totales y de ciertos ácidos grasos de los ejemplares. Dicha dieta suplementada produjo además cambios importantes en las especies microbianas intestinales de dichos organismos. La realización de un análisis de componentes principales (ACP) confirmó el efecto de la dieta probiótica en los ejemplares, sobre su microbiota y sobre a los niveles de ácidos grasos analizados

Effects on intestinal microbiota and immune genes of solea senegalensis after suspension of the administration of shewanella putrefaciens Pdp11

The interaction host-intestinal microbiota is essential for the immunological homeostasis of the host. Probiotics, prebiotics and synbiotics are promising tools for the manipulation of the intestinal microbiota towards beneficial effects to the host. The objective of this study was to evaluate the modulation effect on the intestinal microbiota and the transcription of genes involved in the immune response in head kidney of Solea senegalensis after administration of diet supplemented with the prebiotic alginate and the probiotic Shewanella putrefaciens Pdp11 CECT 7627 (SpPdp11). The results showed higher adaptability to dietary changes in the intestinal microbiota of fish fed diet with alginate and SpPdp11 together compared to those fish that received an alginate-supplemented diet. The alginate-supplemented diet produced up-regulation of genes encoding proteins involved in immunological responses, such as complement, lysozyme G and transferrin, and oxidative stress, such as NADPH oxidase and glutation peroxidase. On the other hand, the administration of alginate combined with SpPdp11 resulted in a significant increase of the transcription of genes encoding for glutation peroxidase and HSP70, indicating a potential protective effect of SpPdp11 against oxidative stress. In addition, these effects were maintained after the suspension of the probiotic treatment. The relationship between the modulation of the intestinal microbiota and the expression of genes with protective effect against the oxidative stress was demonstrated by the Principal Components Analysis.En prensa3,322