The undertakers: Learning from an unusual work experience placement

Providing care for dying patients and their families is a fundamental part of general practice. There are barriers to the effective provision of such care at both individual and organisational levels (Mitchell et al., 2016), as well as cultural and social barriers to discussing the end of life and dying (www.dyingmatters.org). The extent to which we are exposed to death during training is highly variable, and the way that we are educated to cope with both the practicalities and the emotional aspects of death and dying receives little attention in undergraduate or postgraduate training

Targeted modulation of tropoelastin structure and assembly

Tropoelastin, as the monomer unit of elastin, assembles into elastic fibers that impart strength and resilience to elastic tissues. Tropoelastin is also widely used to manufacture versatile materials with specific mechanical and biological properties. The assembly of tropoelastin into elastic fibers or biomaterials is crucially influenced by key submolecular regions and specific residues within these domains. In this work, we identify the functional contributions of two rarely occurring negatively charged residues, glutamate 345 in domain 19 and glutamate 414 in domain 21, in jointly maintaining the native conformation of the tropoelastin hinge, bridge and foot regions. Alanine substitution of E345 and/or E414 variably alters the positioning and interactive accessibility of these regions, as illustrated by nanostructural studies and detected by antibody and cell probes. These structural changes are associated with a lower propensity for monomer coacervation, cross-linking into morphologically and functionally atypical hydrogels, and markedly impaired and abnormal elastic fiber formation. Our work indicates the crucial significance of both E345 and E414 residues in modulating specific local structure and higher-order assembly of human tropoelastin

Quantitative evaluation of polymer gel dosimeters by broadband ultrasound attenuation

Ultrasound has been examined previously as an alternative readout method for irradiated polymer gel dosimeters, with authors reporting varying dose response to ultrasound transmission measurements. In this current work we extend previous work to measure the broadband ultrasound attenuation (BUA) response of irradiated PAGAT gel dosimeters, using a novel ultrasound computed tomography system

Management and drivers of change of pollinating insects and pollination services. National Pollinator Strategy: for bees and other pollinators in England, Evidence statements and Summary of Evidence

These Evidence Statements provide up-to-date information on what is known (and not known) about the status, values, drivers of change, and responses to management of UK insect pollinators (as was September 2018). This document has been produced to inform the development of England pollinator policy, and provide insight into the evidence that underpins policy decision-making. This document sits alongside a more detailed Summary of Evidence (Annex I) document written by pollinator experts. For information on the development of the statements, and confidence ratings assigned to them, please see section ‘Generation of the statements’ below. Citations for these statements are contained in the Summary of Evidence document

Large scale experiments on beach evolution induced by bichromatic wave groups with varying group period

New large scale experimental data have been presented showing the wave group influence on beach morphodynamics at the surf and swash zones. Bichromatic wave conditions were generated varying the modulation bandwidth but keeping the wave energetic content constant within the experimentation limits. The wave group influence in the surf zone is observed in the form of the cross-shore location of the breaker bar respect to the initial still water level (SWL) location, which has been shown to increase as increases the wave group period. This influence is explained in terms of differences on the surf zone width induced by the varying wave group periods. In the swash zone, time dependent bed level elevation measurements were done using a newly developed conductivity technique, the CCM+ system. Bed level time variations at the swash zone have shown to be composed of a long scale trend and bed level oscillations of shorter frequencies related to the wave group forcing. A good spectral correlation has been found between the water surface elevation and bed level variation at the wave group period for every bichromatic component indicating an important influence of wave groups on the swash zone morphodynamics

Methodological Diversity in Citizen Science Mosquito Surveillance: A Scoping Review

Global concern regarding mosquito-borne disease emergence and re-emergence has driven the development of citizen science mosquito surveillance initiatives. Although these initiatives have shown great potential to assist local health authorities, ensuring outcomes are translatable to improved public health policy and practice remains challenging. Here we present a summary of citizen science mosquito surveillance programs worldwide, their focus, strategies, and outcomes, with a view to how best to apply this approach in their local areas. A scoping review of studies and reports was conducted through systematic search on electronic databases (Scopus, PubMed, Web of Science, and Google Scholar), grey literature, and other documents listed in the references of selected articles. A total of 33 citizen science studies included in this review described 29 citizen science mosquito surveillance projects operating in 16 countries, besides three programs with wide geographic coverage. The selected programs focused on different strategies and methods according to their local and national contextual needs. The majority of the programs reported being free or low in cost, and amenable to participants. Also, citizen scientists valued the opportunity to actively contribute to a scientific activity in which they saw value. Local and national programs have been successful in involving the broader public and yielding data on mosquito populations. However, to ensure the best public health outcomes, sustainability, and scalability, there is a need to continue engaging with stakeholders, including community members, researchers, public health agents, industry, and policymakers, and to bridge existing collaborations across different sectors

Subtle balance of tropoelastin molecular shape and flexibility regulates dynamics and hierarchical assembly

The assembly of the tropoelastin monomer into elastin is vital for conferring elasticity on blood vessels, skin, and lungs. Tropoelastin has dual needs for flexibility and structure in self-assembly. We explore the structure-dynamics-function interplay, consider the duality of molecular order and disorder, and identify equally significant functional contributions by local and global structures. To study these organizational stratifications, we perturb a key hinge region by expressing an exon that is universally spliced out in human tropoelastins. We find a herniated nanostructure with a displaced C terminus and explain by molecular modeling that flexible helices are replaced with substantial β sheets. We see atypical higher-order cross-linking and inefficient assembly into discontinuous, thick elastic fibers. We explain this dysfunction by correlating local and global structural effects with changes in the molecule’s assembly dynamics. This work has general implications for our understanding of elastomeric proteins, which balance disordered regions with defined structural modules at multiple scales for functional assembly.United States. Office of Naval Research (Presidential Early Career Award for Scientists and Engineers)National Institutes of Health (U.S.) (U01 EB014976